Impact of biochar amendment on the abundance and structure of diazotrophic community in an alkaline soil

Liu, Xiaoyu; Liu, Cheng; Gao, Wenhui; Xue, Chen; Guo, Zonghao; Jiang, Li; Li, Feng; Liu, Yuan

doi:10.1016/j.scitotenv.2019.06.293

Cited by 56 publications

(30 citation statements)

References 60 publications

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“…It should be noted that, various studies showed that soil pH is a dominant factor for the change in bacterial abundance (Yao et al, 2017b;Liu et al, 2019;Zheng et al, 2019;Sun et al, 2020). However, test soil in this study was alkaline, and biochar application resulted no changes in soil pH and bacterial abundance.…”

Section: Effects Of Biochar On the Abundances Of Soil Bacteria And Fungicontrasting

confidence: 50%

“…Soil microorganisms play a pivotal role in processes such as nutrient cycling and mineralization ( Zheng et al, 2016 ). Changes in microbial community structure, owing to sensitivity to environmental conditions, might indicate the potential effects of biochar ( Liu et al, 2019 ). Soil bacteria and fungi play an important role in soil nutrient transformation and can decompose recalcitrant matter such as cellulose and lignin ( Gul et al, 2015 ; Yao et al, 2017a ).…”

Section: Introductionmentioning

confidence: 99%

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Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Gao

Guo

et al. 2021

Front. Microbiol.

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Biochar as a soil amendment has been regarded as a promising way to improve soil fertility. However, the response of microbial community after biochar and biochar compound fertilizer (BCF) application has not been thoroughly elucidated. This study evaluated the changes in abundance and composition of bacterial and fungal communities using quantitative real-time PCR (qPCR) and Illumina MiSeq amplicon sequencing. The field experiment ran for 3 years and comprised five treatments: chemical fertilizer as control (CK), straw-returning combined with chemical fertilizer (CS), low biochar application combined with chemical fertilizer (LB), high biochar application combined with chemical fertilizer (HB) and BCF. The results showed that biochar amendment results no changes in the abundance and diversity of bacteria in the bulk and rhizosphere soils. However, the abundance of soil fungi was significantly increased by biochar amendment (LB and HB). LB treatment significantly increased the fungal alpha diversity, while there was no significant change under HB. Furthermore, the dominant bacterial phyla found in the samples were Proteobacteria, Actinobacteria, and Acidobacteria. Biochar addition increased the relative abundance of Actinobacteria in both bulk and rhizosphere soils. The dominant fungal phyla were Ascomycota, Mortierellomycota, and Basidiomycota. The relative abundance of Ascomycota significantly decreased, but Mortierellomycota significantly increased in LB and HB. In addition, redundancy analysis indicated that the changes in bacterial and fungal communities are associated with soil properties such as SOC and TN, which are crucial contributors in regulating the community composition. This study is expected to provide significant theoretical and practical knowledge for the application of biochar in agricultural ecosystem.

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Section: Effects Of Biochar On the Abundances Of Soil Bacteria And Fungicontrasting

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Gao

Guo

et al. 2021

Front. Microbiol.

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“…Diazotrophs are sensitive to soil physiochemical properties, including soil pH (Wang Y.S. et al, 2017;Fan et al, 2018), soil organic carbon (SOC) content (Collavino et al, 2014;Yang et al, 2019), C and N availability Hu et al, 2019;Yang et al, 2021), phosphorus content (Tang et al, 2017), soil moisture (Penton et al, 2016), and C:N ratio Liu et al, 2019). However, the effects of soil physiochemical properties on the diazotrophs have been variable.…”

Section: Introductionmentioning

confidence: 99%

Straw Mulching and Nitrogen Fertilization Affect Diazotroph Communities in Wheat Rhizosphere

Chen

Xiang

et al. 2021

Front. Microbiol.

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Diazotrophs that carry out the biological fixation of atmospheric dinitrogen (N2) replenish biologically available nitrogen (N) in soil and are influenced by the input of inorganic and organic substrates. To date, little is known about the effects of combined organic substrate addition and N fertilization on the diazotroph community composition and structure in purple soils. We investigated the effects of N fertilization and straw mulching on diazotroph communities by quantifying and sequencing the nifH gene in wheat rhizosphere. The abundance and richness of diazotrophs were greater the higher the fertilization level in the mulched treatments, whereas in the nonmulched treatments (NSMs), richness was lowest with the highest N fertilization level. The abundance and α-diversity of diazotrophs correlated with most of the soil properties but not with pH. At the genus level, the relative abundances of Azospirillum, Bacillus, and Geobacter were higher in the NSMs and those of Pseudacidovorax, Skermanella, Azospira, Paraburkholderia, Azotobacter, Desulfovibrio, Klebsiella, and Pelomonas in the mulched treatments. The differences in community composition between the mulched and the NSMs were associated with differences in soil temperature and soil organic carbon and available potassium contents and C:N ratio. Overall, straw mulching and N fertilization were associated with changes in diazotroph community composition and higher abundance of nifH gene in alkaline purple soils.

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“…With the development of molecular biology technology, the nifH gene (a structural gene of nitrogenase reductase) is widely used as a molecular biomarker to detect the diazotrophic microbes in soil. It has been reported that diazotrophs are affected by various factors (abiotic/biotic factors), such as different kinds of fertilizer treatments [8][9][10][11], soil nutrients and pH [12,13], geographical scale [14], application of Mo and biochar [15,16] and crop types and cultivation history [17] and so on.…”

Section: Introductionmentioning

confidence: 99%

“…While the characteristics of diazotrophic bacteria in the black soil area of Northeast China has not been reported. In addition, most related studies were mainly based on one-year field treatment or potted plant tests in laboratories [15,16,27,28]. Longterm positioning of fertilization conditions was regarded to achieve stabile soil nutrients, enzyme activity and microbial community structure [17,29].…”

Section: Introductionmentioning

confidence: 99%

Negative impacts of excessive nitrogen fertilization on the abundance and diversity of diazotrophs in black soil with monocropping maize

Chen¹,

Li²,

Shi³

et al. 2020

Preprint

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Background: Excessive nitrogen fertilizer input and low nitrogen fertilizer use efficiency in maize in China are serious ecological and economic problems, which might affect the procedures in the nitrogen cycle. To reveal the effects of long-term excessive nitrogen fertilization on diazotrophs in maize rhizosphere and bulk soil, we performed a long-term (five-year) N-input experiment (N rates from 0 to 300 kg N ha -1 ) in black soil maize in northeast China. The effect of N fertilizer application rates on the abundance, structure and compositions of diazotrophic community in both the bulk soil and rhizosphere of maize were investigated by Real-time quantitative PCR and high-throughput sequencing, and a structural equation model was constructed based on this study.Results: 1) Excessive N fertilization significantly reduced the abundance and diversity of diazotrophs. 2) The accumulation of Sphingobium was correlated positively with soil nitrate concentration and soil EC, and negatively with soil pH. The contrast correlation was found in Burkholderia . 3) Diazotrophs were enriched in maize rhizosphere, but the diversity and compositions of diazotrophic community were less affected by maize rhizosphere effect. 4) The enriched Bradyrhizobium and Methylobacterium in maize rhizosphere showed a significant positive correlation with of maize plant biomass. Conclusions: Our results suggest that through affecting soil pH, nitrate and EC values, long-term excessive N input increase Sphingobium accumulation and reduce the abundance of beneficial diazotrophs such as Bradyrhizobium and Burkholderia ，which contribute to the decreased nitrogen use efficiency.

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Impact of biochar amendment on the abundance and structure of diazotrophic community in an alkaline soil

Cited by 56 publications

References 60 publications

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Different Responses of Soil Bacterial and Fungal Communities to 3 Years of Biochar Amendment in an Alkaline Soybean Soil

Straw Mulching and Nitrogen Fertilization Affect Diazotroph Communities in Wheat Rhizosphere

Negative impacts of excessive nitrogen fertilization on the abundance and diversity of diazotrophs in black soil with monocropping maize

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