Impact of biosolids amendment and wastewater effluent irrigation on enteric antibiotic-resistant bacteria – a greenhouse study

Mays, Catherine; Garza, Gabriela L.; Waite‐Cusic, Joy; Radniecki, Tyler S.; Navab-Daneshmand, Tala

doi:10.1016/j.wroa.2021.100119

Cited by 9 publications

(8 citation statements)

References 26 publications

(29 reference statements)

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“…All glassware used for the synthesis of AgNPs were cleaned with aqua regia (3-parts in volume of hydrochloric acid and 1-part in volume of nitric acid) prior to their use. AgNPs were synthesized based on the co-reduction method using sodium citrate and tannic acid (29). Briefly, a 100 mL aqueous solution of 5 mM trisodium citrate and 0.25 mM of tannic acid were brought to a boil at 100 °C under vigorous stirring.…”

Section: Experimental Protocolsmentioning

confidence: 99%

“…The antimicrobial activities of AgNPs, PpIX-AgNPs and PEI-PpIX-AgNPs were examined against a MRSA strain (BAA 44) purchased from ATCC and a wild-type MDR E. coli strain (accession number PRJNA806466). The MDR E. coli was isolated from Class B biosolids amended soil 29 . Details of the MDR E. coli sequence protocol and data analysis are provided in the ESI.…”

Section: Bacterial Inactivation Experimentsmentioning

confidence: 99%

“…The MDR E. coli was isolated from Class B biosolids amended soil. 29 Details of the MDR E. coli sequence protocol and data analysis are provided in the ESI. † It was confirmed to be resistant to tetracycline, gentamicin, ampicillin, sulfamethoxazole-trimethoprim and ciprofloxacin using the CLSI M100 Performance Standards.…”

Section: Experimental Protocolsmentioning

confidence: 99%

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Influence of silver ion release on the inactivation of antibiotic resistant bacteria using light-activated silver nanoparticles

et al. 2022

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Section: Experimental Protocolsmentioning

confidence: 99%

Section: Bacterial Inactivation Experimentsmentioning

confidence: 99%

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Influence of silver ion release on the inactivation of antibiotic resistant bacteria using light-activated silver nanoparticles

et al. 2022

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“…Live ARBs have an opportunity to interact with soil microbiota and spread ARGs into the soil bacterial community via the horizontal gene transfer (Woegerbauer et al, 2020). With the annual application of biosolid fertilizers, the ARGs from biosolids may persist (Mays et al, 2021) or accumulate in the soil (Xie et al, 2016;Zhuang et al, 2021), which may continue to disseminate ARGs to humans potentially through various potential ways including food chains…”

Section: Co-occurrence Patterns Between Args and Bacterial Communitiesmentioning

confidence: 99%

Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Kang

Naushad

Hartke

et al. 2022

Front. Food. Sci. Technol.

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Dissemination of microorganisms with antimicrobial resistance genes (ARGs) through the food chain has been recognized as a growing public health concern worldwide. Biosolids, a product of wastewater treatment process, have been used as fertilizers in agriculture globally and have also been considered as a potential source of pathogens and ARGs for horizontal transfer across various environments. This study characterized antibiotic resistomes and microbiota in 24 biosolids samples collected from two Canadian waste water treatment plants in different cities in 2009 and 2019. The ARGs were detected using a qPCR array kit, and microbiota was analyzed using 16S ribosomal RNA gene amplicon sequencing. Furthermore, correlation analysis of ARG abundance and bacterial genera abundance was explored to predict potential hosts of ARGs. Seventy-one of 84 ARGs were detected in at least one or more samples with 12 ARGs being detected in all samples. Antibiotic resistomes did not show a statistically significant distinction between different collection years, sites, or year and site combined in principle coordinate analysis. The microbiota communities were significantly different between samples collected in different years, sites, or year and site combined. In total 34 phyla were detected with 13 genera among the top three phyla were typically related to the human gut microbiota and seven of them showing strong correlation with ARGs related to aminoglycoside and beta-lactam resistance. This study provides valuable baseline information and consistent trend on ARGs and bacterial communities in biosolid fertilizers in Canada, indicating that the biosolid fertilizer could potentially be a source of ARGs in the agricultural soils and may leading to potential contamination of plant-based food chains.

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“…Of importance are critical priority pathogens defined by the World Health Organization, including extended-spectrum beta-lactamases (ESBL)-producing and carbapenem-resistant Enterobacteriaceae (WHO, 2017). Globally, treated or untreated wastewater and biosolids are used for irrigation and soil amendment in agriculture, making soil and food crops potential conduits for human exposure to pathogenic ARB (Mays et al, 2021). Further, there are reports of presence and dissemination of ARB and their determinant genes in environmental reservoirs, such as rivers, lakes, and sediments associated with wastewater discharge (Logan et al, 2020;Quintela-Baluja et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic resistance profile of E. coli isolates in 17 municipal wastewater utilities across Oregon

Khorshidi-Zadeh¹,

Yiu²,

Nguyen³

et al. 2021

Preprint

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Wastewater treatment utilities are considered one of the main sources and reservoirs of antimicrobial resistance. The objective of this study was to determine the diversity and prevalence of antibiotic-resistant Escherichia coli in wastewater treatment systems across the state of Oregon. Influent, secondary effluent, final effluent, and biosolids were collected from 17 wastewater treatment utilities across Oregon during the winter and summer seasons of 2019 and 2020 (n = 246). E. coli strains were recovered from samples by culturing on mTEC, followed by confirmation with MacConkey with MUG agar plates. Antibiotic susceptibility of 1143 E. coli isolates against 8 antibiotics were determined, and resistance profiles and indices were analyzed between utilities, seasons, and flows. Antibiotic resistance phenotypes were detected in 31.6% of the collected E. coli isolates. Among those antibiotic-resistant E. coli isolates, multi-drug resistance (i.e., resistance to three or more classes of antibiotics) was harbored by 27.7% with some strains showing resistance to up to six classes of antibiotics. The most prevalent resistance was to ampicillin (n = 207) and the most common combinations of multi-drug resistance included simultaneous resistances to ampicillin, streptomycin, and tetracycline (n = 49), followed by ampicillin, streptomycin, and sulfamethoxazole/trimethoprim (n = 46). Significant correlations were observed between resistance to sulfamethoxazole/trimethoprim and resistances to ampicillin, ciprofloxacin, and tetracycline (p < 0.001). A small percentage (1.1%) of the E. coli isolates displayed extended-spectrum beta lactamase (ESBL) activity and a single isolate carried resistance to imipenem. Compared to wastewater influent, ciprofloxacin resistance was significantly more prevalent in biosolids (p <0.05) and tetracycline resistance was significantly lower in effluent (p <0.05). Seasonal impact on antibiotic-resistant E. coli in wastewater influent was observed through significantly higher multiple antibiotic resistance (MAR) index, ampicillin resistance prevalence, and ciprofloxacin resistance prevalence in summer compared to winter (p < 0.05). This state-wide study confirms the widespread distribution of antibiotic-resistant, multi-drug resistant, and extended-spectrum beta lactamase-producing E. coli in wastewater systems across different flows and seasonal variations, making them the recipients, reservoirs, and sources of antimicrobial resistance.

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Impact of biosolids amendment and wastewater effluent irrigation on enteric antibiotic-resistant bacteria – a greenhouse study

Cited by 9 publications

References 26 publications

Influence of silver ion release on the inactivation of antibiotic resistant bacteria using light-activated silver nanoparticles

Influence of silver ion release on the inactivation of antibiotic resistant bacteria using light-activated silver nanoparticles

Antibiotic resistomes and microbial communities in biosolid fertilizers collected from two Canadian wastewater treatment plants in a 10-years interval-potential risks to food chains?

Antibiotic resistance profile of E. coli isolates in 17 municipal wastewater utilities across Oregon

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