Impact of Body Mass Index on Outcomes of In Vitro Fertilization/Intracytoplasmic Sperm Injection Among Polycystic Ovarian Syndrome Patients

Cui, Na; Wang, Huixia; Wang, Wei; Zhang, Jie; Xu, Yueming; Jiang, Lei; Yang, Aimin; Hao, Guimin

doi:10.1159/000447873

Cited by 26 publications

(49 citation statements)

References 58 publications

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“…NO is an important factor determining IVF success rates [59]. The report is of utmost importance as a recent study has reported that the IVF success rates in obese PCOS women is low on account of higher miscarriage risk [1]. Hence, NOx insufficiency in PCOS women has to be addressed at an early level.…”

Section: Discussionmentioning

confidence: 99%

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Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Krishna

Joseph

Thomas

et al. 2017

Cell Physiol Biochem

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Background: Though oxidative stress is associated with Polycystic Ovary Syndrome (PCOS), the status of nitric oxide is still unclear. Nitric Oxide (NO) plays pivotal roles in many physiological functions which are compromised in PCOS. Our recent study reveals lowered T-regulatory cells (Tregs) in PCOS, and Treg generation is known to be regulated by NO levels. However concrete evidences are lacking on mechanisms modulating NO levels under PCOS. Methods: This is a retrospective case-control cohort study, comprised of PCOS women (N=29) and normal menstruating women as controls (N=20). We analysed NOx (nitrite+nitrate) and hydrogen peroxide (H₂O₂) concentrations, transcript levels of endothelial nitric oxide synthase (eNOS)/inducible nitric oxide synthase (iNOS) and arginine modulators, hydrogen peroxide regulators in the cohort. Results: PCOS women showed reduced plasma NOx(nitrate+nitrite) and H₂O₂ compared to controls. We report reduction in transcript levels of iNOS/NOS2 and eNOS/NOS3 in PCOS peripheral blood. The transcripts involved in arginine bioavailability: Argininosuccinate lyase (ASL), Solute Carrier Family1, member 7 (SLC7A1) and Arginase 1 (ARG1) and Asymmetric Dimethyl Arginine (ADMA) metabolism: Protein arginine methyltransferase 1 (PRMT1) and Dimethylarginine dimethylaminohydrolase 2 (DDAH2) also showed differential expression. H₂O₂ concentration in PCOS women was also found to be reduced. The reduction can be attributed to increase in catalase levels as a consequence of the body’s effort to alleviate the oxidative burden in the system. Conclusion: Our study advocates that PCOS women have lowered NO due to reduced iNOS/eNOS expression, low H₂O₂, high ADMA synthesis and reduced arginine bioavailability. An in-depth analysis of redox biology of PCOS to open up potential therapeutic strategies is highly recommended.

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Section: Discussionmentioning

confidence: 99%

“…PCOS is ranked as the most common cause of female infertility on a global scale. Further deteriorating the scenario PCOS patients have low IVF/ICSI success rates [1] and high incidence of ovarian hyperstimulation syndrome (OHSS) [2]. …”

Section: Introductionmentioning

confidence: 99%

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Krishna

Joseph

Thomas

et al. 2017

Cell Physiol Biochem

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“…Our data demonstrate that changes in serum proteins are related to progesterone in PCOS patients, and progesterone improves ovarian functions by upregulating EREG, inhibin βA, IDE, PDGF-D and KNG1 expression. The accumulated evidences show that PCOS is one of the major causes leading to infertility [1, 36-37]. Based on the complexity of hormone disorders in PCOS patients, such as high estrogen, high androgen, or low progesterone, as well as the combination of the 2 or 3 of the hormones [38], although the clinical treatment strategy now is mainly by anti-estrogen with clomiphene citrate to induce ovulation and anti-androgen with flutamide to alleviate hyperandrogenism, we propose that progesterone supplementation in combination with routine treatments may improve the therapeutic effect in PCOS patients, and prevent the occurrence of abortion and premature delivery, especially patients with low progesterone level.…”

Section: Discussionmentioning

confidence: 99%

Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level

Zheng

Zhou

Cui

et al. 2018

Cell Physiol Biochem

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Background/Aims: Polycystic ovary syndrome (PCOS), characterized by female infertility and metabolic abnormalities, is one of the most common endocrine disorders. The etiology of PCOS remains unknown. The comprehensive analysis of protein alterations in PCOS patients is meaningful for identifying diagnostic biomarkers of PCOS. Here, we explored the clinical value of serum proteins as novel biomarkers to detect PCOS with low progesterone level. Methods: A total of 43 patients with PCOS and 30 healthy women were enrolled. Protein array was used to detect the variations of serum proteins between PCOS patients and healthy women. The level of five serum proteins was further confirmed by ELISA and western blot. The human ovarian granulosa cells (KGN) was cultured to examine the underlying mechanism of PCOS. CCK8 assay and western blot were carried out to evaluate the alterations in proliferative ability, TUNEL assay and DAPI staining to detect the apoptosis of KGN cells. Results: Among the 507 proteins, we identified 76 differentially expressed serum proteins (≧1.5 fold), with 40 elevated and 36 decreased proteins. Moreover, 47 proteins were newly reported in PCOS. The alterations in the five significantly decreased proteins (EREG, inhibin βA, IDE, PDGF-D and KNG1) were further confirmed by ELISA and western blot. The level of these proteins were directly associated with the low progesterone, and the expression could be upregulated by progesterone. EREG and inhibin βA also promoted the proliferation and inhibited the apoptosis of ovarian granulosa cells. Conclusion: The study highlights that serum proteins are differentially expressed in PCOS patients and healthy women, and EREG and inhibin βA levels are upregulated by progesterone, which are correlated with ovarian functions. The study suggests that EREG and inhibin βA may be applied as novel potential biomarkers for PCOS with low progesterone level.

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“…Lin and Lujan [8] reviewed the diet and exercise of PCOS women and found that there was no significant difference in exercise amount between PCOS women and healthy women, but PCOS women were characterized by high-calorie diet, excessive intake of saturated fatty acid and lack of dietary fiber. After receiving ART assisted pregnancy therapy, the clinical pregnancy rate and live birth rate of obese PCOS women were significantly lower than those of normal weight PCOS patients, and the risk of miscarriage was increased [9,10]. Therefore, it was very important to control BMI in the optimal range before pregnancy assistance to improve the success rate of ART.…”

Section: Exercise or Combination With Other Methods Improve Reproductmentioning

confidence: 99%

Effects of Exercise Intervention on the Improvement of Polycystic Ovary Syndrome

Wang¹,

Zhang²,

Liu³

2020

Polycystic Ovarian Syndrome

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Polycystic ovary syndrome (PCOS) is a complex endocrine and metabolic disease characterized by hyperandrogenism and insulin resistance. It results in irregular menstruation, obesity and even infertility, which influences badly physical and mental health of the patients. Now, there is no effective drug for treating PCOS and the preferred program is lifestyle modification, including diet control, regular exercise and behavior therapy. Therefore, in the review, we summarize the study progress concerning the effects of lifestyle intervention on the metabolic, reproductive and psychological dysfunctions of PCOS patients and analyze the corresponding mechanisms in these processes. It can radically reduce the factors of PCOS occurrence and development, while providing valuable information for the prevention and treatment of PCOS and is helpful for further research.

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Impact of Body Mass Index on Outcomes of In Vitro Fertilization/Intracytoplasmic Sperm Injection Among Polycystic Ovarian Syndrome Patients

Cited by 26 publications

References 58 publications

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Impaired Arginine Metabolism Coupled to a Defective Redox Conduit Contributes to Low Plasma Nitric Oxide in Polycystic Ovary Syndrome

Novel Serum Biomarkers Detected by Protein Array in Polycystic Ovary Syndrome with Low Progesterone Level

Effects of Exercise Intervention on the Improvement of Polycystic Ovary Syndrome

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