Impact of cadmium and nickel on ion homeostasis in the yeast <i>Schizosaccharomyces pombe</i>

Požgajová, Miroslava; Navrátilová, Alica; Árvay, Július; Ďúranová, Hana; Trakovická, Anna

doi:10.1080/03601234.2019.1673613

Cited by 12 publications

(12 citation statements)

References 48 publications

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“…What is more, phenolic compounds show an additional tendency to chelate metallic ions through hydroxyl and carboxyl groups, as well as participate in pathways of cell wall lignification, causing the formation of a thicker mechanical barrier that restricts the penetration of HMs responsible for oxidative damage [7,9,16]. Therefore, modulation of the particular antioxidants production and accumulation depending on the stressor presence can be used to anticipate the direction of metabolism remodeling under HM treatment.The toxicity of HMs applied separately has been intensively studied in taxonomically diversified organisms [8,17,18]. However, the impact of co-treatment with different ions on cellular homeostatic pathways still remains to be elucidated.…”

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confidence: 99%

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Ecotype-Specific Pathways of Reactive Oxygen Species Deactivation in Facultative Metallophyte Silene vulgaris (Moench) Garcke Treated with Heavy Metals

2020

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This research aimed to indicate mechanisms involved in protection against the imbalanced generation of reactive oxygen species (ROS) during heavy metals (HMs) exposition of Silene vulgaris ecotypes with different levels of metal tolerance. Specimens of non-metallicolous (NM), calamine (CAL), and serpentine (SER) ecotypes were treated in vitro with Zn, Pb, and Cd ions applied simultaneously in concentrations that reflected their contents in natural habitats of the CAL ecotype (1× HMs) and 2.5- or 5.0-times higher than the first one. Our findings confirmed the sensitivity of the NM ecotype and revealed that the SER ecotype was not fully adapted to the HM mixture, since intensified lipid peroxidation, ultrastructural alternations, and decline in photosynthetic pigments’ content were ascertained under HM treatment. These changes resulted from insufficient antioxidant defense mechanisms based only on ascorbate peroxidase (APX) activity assisted (depending on HMs concentration) by glutathione-S-transferase (GST) and peroxidase activity at pH 6.8 in the NM ecotype or by GST and guaiacol-type peroxidase in the SER one. In turn, CAL specimens showed a hormetic reaction to 1× HMs, which manifested by both increased accumulation of pigments and most non-enzymatic antioxidants and enhanced activity of catalase and enzymes from the peroxidase family (with the exception of APX). Interestingly, no changes in superoxide dismutase activity were noticed in metallicolous ecotypes. To sum up, the ROS scavenging pathways in S. vulgaris relied on antioxidants specific to the respective ecotypes, however the synthesis of polyphenols was proved to be a universal reaction to HMs.

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confidence: 99%

“…The toxicity of HMs applied separately has been intensively studied in taxonomically diversified organisms [8,17,18]. However, the impact of co-treatment with different ions on cellular homeostatic pathways still remains to be elucidated.…”

mentioning

confidence: 99%

Ecotype-Specific Pathways of Reactive Oxygen Species Deactivation in Facultative Metallophyte Silene vulgaris (Moench) Garcke Treated with Heavy Metals

2020

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“…Similarly, growth rate inhibition of S. pombe was reported also after addition of the other toxic cations e.g. Cd (Clemens, 2003), As (Salgado, 2012) or Ni (Pozgajova et al 2019). Significant drop in the cell growth was observed at concentration 250 µM.…”

Section: Effect Of Aluminium Ions On the Growth Rate Of Cell Culture mentioning

confidence: 62%

Cytotoxic effect of aluminium ions on unicellular eukaryotic organism

Borotová

Drobný

Navrátilová

et al. 2019

Acta fytotechn zootechn

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Licensed under a Creative Commons Attribution 4.0 International License Aluminium is abundant in nature, food, or water and thus its exposition is part of everyday life. However, overexposure can result in cellular malfunctions. Therefore, the aim of this study was to investigate the effects of aluminium on eukaryotes, with the use of Schizosaccharomyces pombe as model organism. Spectrophotometry at OD 600 , inductively-coupled plasma optical emission spectroscopy (ICP-OES) and microscopy techniques were used to analyse aluminium responses on the living system. Our results revealed that exposition of increasing aluminium concentrations lead to cell growth inhibition in a concentration dependent manner. Furthermore, aluminium incorporation by the cell from media markedly increased with rising Al concentration. Our results indicate that the yeast self-protection system in the presence of lower Al(OH) 3 concentration in the environment avoids to large extent dramatic uptake of aluminium by the cell while cells surrounded by higher aluminium concentrations lose this ability. Supplementation of the growth media with 100 μM Al(OH) 3 doubled the amount of Al in the cell compared to untreated control (232 mg/kg vs. 459 mg/kg), whereas addition of 1 mM Al(OH) 3 caused more than hundred fold increase of intracellular Al content (27,781 mg/kg). Here we also show that high concentrations of aluminium have an impact on cell morphology leading to cell integrity disruption. Findings presented in this study have the ambition to bring more light in an issue of how aluminium mediates impairments of the living organism.

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“…Cd exposure cause misfolding and aggregation of cytosolic proteins in yeast [54]. Cd exposure also leads to metal ion imbalance in yeast cells [55]. Cd inhibits SOD through disturbing protein conformations and ion equilibriums, such as for Zn and Cu [33].…”

Section: Discussionmentioning

confidence: 99%

Protective role of l‐threonine against cadmium toxicity in Saccharomyces cerevisiae

et al. 2021

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Environment and food contamination with cadmium (Cd) can cause serious toxicity, posing a severe threat to agricultural production and human health. However, how amino acids contribute to defenses against oxidative stress caused by Cd in cells is not fully understood. As a model eukaryote with a relatively clear genetic background, Saccharomyces cerevisiae has been commonly used in Cd toxicity research. To gain insight into Cd toxicity and cell defenses against it, 20 amino acids were screened for protective roles against Cd stress in S. cerevisiae. The results showed that threonine (Thr, T) had the strongest protective effect against Cd‐induced mortality and membrane damage in the cells. Compared to the antioxidant vitamin C (VC), Thr exhibited a higher efficacy in restoring the superoxide dismutase (SOD) activity that was inhibited by Cd but not by H2O2 in vivo. Thr exhibited evident DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) activity but weak ABTS (2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐9 sulfonic acid)) scavenging activity, giving it a weaker effect against Cd‐induced lipid peroxidation and superoxide radical O2−, compared to VC. More importantly, compared to the chelating agent EDTA, Thr showed stronger chelation of Cd, giving it a stronger protective effect on SOD against Cd than VC in vitro. The results of the in vivo and in vitro experiments revealed that the role Thr plays in cell defenses against Cd may be attributed to its protection of the SOD enzyme, predominantly through the preferential chelation of Cd. Our results provide insights into the protective mechanisms of amino acid Thr that ameliorate Cd toxicity and suggest that a supplement of Thr might help to reduce Cd‐induced oxidative damage.

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Impact of cadmium and nickel on ion homeostasis in the yeast Schizosaccharomyces pombe

Cited by 12 publications

References 48 publications

Ecotype-Specific Pathways of Reactive Oxygen Species Deactivation in Facultative Metallophyte Silene vulgaris (Moench) Garcke Treated with Heavy Metals

Ecotype-Specific Pathways of Reactive Oxygen Species Deactivation in Facultative Metallophyte Silene vulgaris (Moench) Garcke Treated with Heavy Metals

Cytotoxic effect of aluminium ions on unicellular eukaryotic organism

Protective role of l‐threonine against cadmium toxicity in Saccharomyces cerevisiae

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