Impact of cadmium toxicity on cartilage loss in a 3D in vitro model

Eduviges, Zamudio Cuevas Yessica; Martínez‐Nava, Gabriela Angélica; Reyes-Hinojosa, Daniel; Mendoza-Soto, Luzia; Fernández‐Torres, Javier; López‐Reyes, Alberto; Olivos-Meza, Anell; Armienta-Hernández, María Aurora; Ruíz-Huerta, Esther Aurora; González-Guadarrama, María de Jesús; Sandoval, Bertha Vargas; Landa-Solís, Carlos; Sánchez-Sánchez, Roberto; Suárez-Ahedo, Carlos; Lozada, Carlos; Gutiérrez‐Ruiz, María Concepción; Clavijo-Cornejo, Denise; Pineda, Carlos; Jacobo-Albavera, Leonor; Domínguez‐Pérez, Mayra; Martínez-Flores, Karina

doi:10.1016/j.etap.2019.103307

Cited by 26 publications

(12 citation statements)

References 17 publications

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“…Third, in addition to ROS, tobacco contains toxic metals such as cadmium (Cd), an element that was not included in this study and that has been shown to promote ECM loss in studies using three-dimensional (3D) chondrocyte cultures. 49 Finally, other oxidative molecules, such as nitric oxide, which are involved in the process of articular cartilage loss, as well as inflammatory molecules, such as interleukins, which contribute to the activation of enzymes that degrade ECM and may be related to the severe OA presented by young smokers in this study, should be investigated.…”

Section: Discussionmentioning

confidence: 99%

Effect of smoking on the redox status of knee osteoarthritis: A preliminary study

Fernández-Torres,

Aztatzi-Aguilar,

Zamudio-Cuevas

et al. 2023

Exp Biol Med (Maywood)

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Even though smoking has been scarcely studied in osteoarthritis (OA) etiology, it is considered a controversial risk factor for the disease. Exposure to tobacco smoke has been reported to promote oxidative stress (OS) as part of the damage mechanism. The aim of this study was to assess whether smoking increases cartilage damage through the generation of OS. Peripheral blood (PB) and synovial fluid (SF) samples from patients with OA were analyzed. The samples were stratified according to smoking habit, Kellgren–Lawrence score, pain, and cotinine concentrations in PB. Malondialdehyde (MDA), methylglyoxal (MGO), advanced protein oxidation products (APOPs), and myeloperoxidase (MPO) were assessed; the activity of antioxidant enzymes such as gamma-glutamyl transferase (GGT), glutathione S-transferase (GST) and catalase (CAT), as well as the activity of arginase, which favors the destruction of cartilage, was determined. When stratified by age, for individuals <60 years, the levels of MDA and APOPs and the activity of MPO and GST were higher, as well as antioxidant system activity in the smoking group (OA-S). A greater degree of pain in the OA-S group increased the concentrations of APOPs and arginase activity ( P < 0.01 and P < 0.05, respectively). Arginase activity increased significantly with a higher degree of pain ( P < 0.01). Active smoking can be an important risk factor for the development of OA by inducing systemic OS in young adults, in addition to reducing antioxidant enzymes in older adults and enhancing the degree of pain and loss of cartilage.

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Section: Discussionmentioning

confidence: 99%

Effect of smoking on the redox status of knee osteoarthritis: A preliminary study

Fernández-Torres,

Aztatzi-Aguilar,

Zamudio-Cuevas

et al. 2023

Exp Biol Med (Maywood)

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“…For instance, bisphenol A was detected not only in the serum of the OA patients, but also in the synovial fluid of knee replacement patients, and exhibited a concentration-dependent antagonistic effect on the protective actions of E2 on chondrocyte, which decreased the NF-kappaB activation and MMP1 expression [ 47 ]. In addition, the exposure of cadmium chloride could reduce the chondrocyte cell viability, increase the expression of the catabolic markers (MMP13, MMP9, MMP3, MMP1) and inflammatory markers (IL-1β and IL-6), and activate the expression of the cartilage extracellular matrix genes (aggrecan and collagen II), and the cadmium contributed the cartilage loss by activating the interleukins through the reactive oxygen species [ 48 ]. Clinical findings supported the harmful effect of cadmium, and cadmium exposure through smoking was positively correlated with the severity of OA [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of candidate genes and chemicals associated with osteoarthritis by transcriptome-wide association study and chemical-gene interaction analysis

Mei,

Zhang,

Chen

et al. 2023

Arthritis Res Ther

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Background Osteoarthritis (OA) is a common degenerative joint disease and causes chronic pain and disability to the elderly. Several risk factors are involved, such as aging, obesity, genetic susceptibility, and environmental factors. We conducted a transcriptome-wide association study (TWAS) and chemical-related gene set enrichment analysis (CGSEA) to investigate the susceptibility genes and environmental factors. Methods TWAS analysis was conducted to identify the susceptibility genes by integrating the summary-level genome-wide association study data of knee OA (KOA) and hip OA (HOA) with the precomputed expression weights from the Genotype-Tissue Expression Project (Version 8). The FUSION software was used for both single-tissue and cross-tissue TWAS, which were combined using an aggregate Cauchy association test. The biological function and pathways of the TWAS genes were explored using the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) databases, and the human cartilage mRNA expression profiles were utilized to validate the TWAS genes. CGSEA analysis was performed to scan the OA-associated chemicals by integrating the TWAS results with the chemical-related gene sets. Results There were 44 and 93 unique TWAS genes identified in 7 and 11 chromosomes for KOA and HOA, respectively, fourteen and four of which showed significantly differential expression in the mRNA profiles, such as CRHR1, LTBP1, WWP2, LMX1B, and PTHLH. OA-related pathways were found in the KEGG and GO analysis, such as TGF-beta signaling pathway, MAPK signaling pathway, hyaluronan metabolic process, and chondrocyte differentiation. Forty-five OA-associated chemicals were identified, including quercetin, bisphenol A, and cadmium chloride. Conclusions Several candidate OA-associated genes and chemicals were identified through TWAS and CGSEA analysis, which expanded our understanding of the relationship between genes, chemicals, and their impact on OA.

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“…Krachler et al ( 40 ) have indicated that cadmium concentration in serum of patients with OA is 0.114 mg/kg, which exceeds the maximum reference limit without occupational exposure. Cadmium can reduce the content of proteoglycan and glycosaminoglycan and inhibit the expression of type II collagen and aggrecan in the extracellular matrix of articular cartilage ( 41 ).…”

Section: Impact Of Eight Trace Elements On Oamentioning

confidence: 99%

“…Epidemiological studies have showed that exposure to cadmium, may stimulate OA progression due to its ability to trigger oxidative stress ( 44 , 105 ). This is because cadmium easily displaces elements such as Fe 2+ and Cu 2+ in the membrane and cytosolic proteins and interacts with the sulfhydryl groups in the mitochondrial membrane proteins and antioxidants, such as catalase, glutathione and superoxide dismutase, thereby promoting the production of reactive oxygen species such as hydrogen peroxide and hydroxyl radicals ( 41 , 45 ). This results in production of interleukin-1β and interleukin-6, which activate matrix metalloproteinases (MMP-1, MMP-3, MMP-9, MMP-13) that destroy the extracellular matrix and affect the expression of aggrecan and type II collagen ( 46 ).…”

Section: Impact Of Eight Trace Elements On Oamentioning

confidence: 99%

The Impact of Trace Elements on Osteoarthritis

Cheng

2021

Front. Med.

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Osteoarthritis (OA) is a progressive degenerative disease characterized by cartilage degradation, synovial inflammation, subchondral sclerosis and osteophyte formation. It has a multifactorial etiology with potential contributions from heredity, endocrine function, abnormal mechanical load and nutrition. Of particular considerations are trace element status. Several trace elements, such as boron and magnesium are essential for normal development of the bone and joint in human. While cadmium correlates with the severity of OA. The present review focuses on the roles of trace elements (boron, cadmium, copper, iron, magnesium, manganese, selenium, zinc) in OA and explores the mechanisms by which they act.

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Impact of cadmium toxicity on cartilage loss in a 3D in vitro model

Cited by 26 publications

References 17 publications

Effect of smoking on the redox status of knee osteoarthritis: A preliminary study

Effect of smoking on the redox status of knee osteoarthritis: A preliminary study

Identification of candidate genes and chemicals associated with osteoarthritis by transcriptome-wide association study and chemical-gene interaction analysis

The Impact of Trace Elements on Osteoarthritis

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