Impact of caspase activation in human spermatozoa

Grunewald, Sonja; Sharma, Rakesh; Paasch, Uwe; Glander, H.‐J.; Agarwal, Ashok

doi:10.1002/jemt.20732

Cited by 54 publications

(52 citation statements)

References 91 publications

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“…Similarly, a negative correlation was observed between Y1 + /PI-sperm with DCF+ sperm. These results are in contrast to the expectation or general hypothesis that increased ROS is positively linked with apoptosis [Barroso et al 2000;Grunewald et al 2009;Paasch et al 2004;Wang et al 2003]. However, this observation reflects the fact that for apoptosis to take place an initial rise in ROS occurs and its deleterious effect is subsequent to this increase in ROS.…”

Section: Discussioncontrasting

confidence: 82%

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Kiani-Esfahani¹,

Bahrami²,

Tavalaee³

et al. 2013

Systems Biology in Reproductive Medicine

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The aim of this study was to determine if there are any associations between impaired chromatin packaging and the origin of reactive oxygen species (ROS) production. Cytosolic ROS, mitochondrial ROS, DNA protamination, and apoptosis were evaluated with dichlorofluoresceindiacetate (DCFH-DA), dihydrorhodamine 123 (DHR123), chromomycin A3 (CMA3), and YO-Pro-1 (Y1)/propidium iodide (PI), respectively, by flow cytometry (FCM) in 40 infertile individuals. Percentages DCF+ and R123+ sperm were positively associated with percentage CMA3+ sperm and negatively associated with percentage apoptotic sperm. No correlation was observed between CMA3+ sperm and the percentage of apoptotic sperm. Under protamination of sperm is not associated with the origin of ROS production, but their relationship may suggest an association with general physiological dysfunction of sperm. Furthermore, under protamination does make sperm prone to apoptosis. Rather, it is likely that apoptosis is induced by ROS production. Considering that these conclusions are derived from correlative analyses, additional studies including an interventive approach are required.

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Section: Discussioncontrasting

confidence: 82%

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Kiani-Esfahani¹,

Bahrami²,

Tavalaee³

et al. 2013

Systems Biology in Reproductive Medicine

View full text Add to dashboard Cite

show abstract

“…[49][50][51] Although many of the reagents that have been shown to induce apoptosis in somatic cells (staurosporine, lipopolysaccharide, 3-deoxy-Dmanno-octulosonic acid (Kdo) and genistein) are ineffective with human spermatozoa, these cells will default to the intrinsic apoptotic pathway in response to oxidative stress. Thus, exposure of human spermatozoa to H 2 O 2 can readily trigger an apoptotic cascade charApoptosis in sperm RJ Aitken and AJ Koppers 38 acterized by the activation of caspase 3 and the appearance of annexin-V binding positivity.…”

Section: Mitochondrial Ros and Apoptosismentioning

confidence: 99%

Apoptosis and DNA damage in human spermatozoa

Aitken¹,

Koppers²

2010

Asian J Androl

292

179

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DNA damage is frequently encountered in spermatozoa of subfertile males and is correlated with a range of adverse clinical outcomes including impaired fertilization, disrupted preimplantation embryonic development, increased rates of miscarriage and an enhanced risk of disease in the progeny. The etiology of DNA fragmentation in human spermatozoa is closely correlated with the appearance of oxidative base adducts and evidence of impaired spermiogenesis. We hypothesize that oxidative stress impedes spermiogenesis, resulting in the generation of spermatozoa with poorly remodelled chromatin. These defective cells have a tendency to default to an apoptotic pathway associated with motility loss, caspase activation, phosphatidylserine exteriorization and the activation of free radical generation by the mitochondria. The latter induces lipid peroxidation and oxidative DNA damage, which then leads to DNA fragmentation and cell death. The physical architecture of spermatozoa prevents any nucleases activated as a result of this apoptotic process from gaining access to the nuclear DNA and inducing its fragmentation. It is for this reason that a majority of the DNA damage encountered in human spermatozoa seems to be oxidative. Given the important role that oxidative stress seems to have in the etiology of DNA damage, there should be an important role for antioxidants in the treatment of this condition. If oxidative DNA damage in spermatozoa is providing a sensitive readout of systemic oxidative stress, the implications of these findings could stretch beyond our immediate goal of trying to minimize DNA damage in spermatozoa as a prelude to assisted conception therapy.

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“…A role of apoptosis in the etiology of spontaneous male infertility has also been suggested by virtue of the excessively high numbers of apoptotic germ cells observed in the testes of some infertile males (Lin et al 1997). In addition, apoptotic markers including caspase activation and phophotidylserine exteriorization have been discovered in the mature spermatozoa of male infertility patients (Barroso et al 2000, Paasch et al 2004, Almeida et al 2009, Grunewald et al 2009, Aitken & Curry 2011. Since apoptosis in mature spermatozoa appears to be critically involved in the induction of a subfertile state, it is this aspect of the apoptotic process that we shall focus on in the first part of this review.…”

Section: Apoptosis In the Testesmentioning

confidence: 99%

Apoptosis in the germ line

Aitken¹,

Findlay²,

Hutt³

et al. 2011

Reproduction

160

102

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Apoptosis is a critical process for regulating both the size and the quality of the male and female germ lines. In this review, we examine the importance of this process during embryonic development in establishing the pool of spermatogonial stem cells and primordial follicles that will ultimately define male and female fertility. We also consider the importance of apoptosis in controlling the number and quality of germ cells that eventually determine reproductive success. The biochemical details of the apoptotic process as it affects germ cells in the mature gonad still await resolution, as do the stimuli that persuade these cells to commit to a pathway that leads to cell death. Our ability to understand and ultimately control the reproductive potential of male and female mammals depends upon a deeper understanding of these fundamental processes.

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Impact of caspase activation in human spermatozoa

Cited by 54 publications

References 91 publications

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Cytosolic and mitochondrial ROS: which one is associated with poor chromatin remodeling?

Apoptosis and DNA damage in human spermatozoa

Apoptosis in the germ line

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