Impact of CD34-dependent Micro Vessel Density on Periapical Odontogenic Cysts

Mathiou, Vasiliki; Tsiambas, Evangelos; Maipas, Sotirios; Thymara, Irene; Peschos, Dimitrios; Lazaris, Andreas C.; Kavantzas, Nikolaos

doi:10.21873/cdp.10200

Cited by 3 publications

(2 citation statements)

References 30 publications

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“…The aim is to assess the angiogenesis process and various neo-angiogenesis levels [13,14]. Other studies assess the MVD in inflammatory diseases such as gingivitis, inflammatory periapical granulomas or cysts [2,6,[15][16][17]. Our study confirms the variability of the microvessels and the fact that the connective tissue had the highest average numbers of vessels.…”

Section: Discussionsupporting

confidence: 72%

Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Roi,

Negruțiu,

Roi

et al. 2023

Biomedicines

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Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion.

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Section: Discussionsupporting

confidence: 72%

Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Roi,

Negruțiu,

Roi

et al. 2023

Biomedicines

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“…A subset of these cystic lesions are characterized by increased neo-angiogenesis leading to a neoplastic-like tissue formation. In these cases, cluster differentiation 34 (CD34) protein expression combined with micro vessel density (MVD) measurement are eligible biomarkers in order for neo-angiogenesis levels to be estimated (38,39). Concerning the HSPs expression profiles in odontogenic cysts in comparison to ameloblastomas, there are limited data.…”

Section: Hsps In Odontogenic Cystsmentioning

confidence: 99%

Heat Shock Proteins’ Expression Profiles in Odontogenic Ameloblastomas and Cysts

MATHIOU,

TSIAMBAS,

MAIPAS

et al. 2023

CDP

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Tumors and cysts with odontogenic origin represent a family of lesions with specific histo-genetic and clinical characteristics. Among them, ameloblastomas are common benign neoplasms, predominantly detected in the anatomic areas of the jaws and also in the mandible and maxilla. Although they are characterized by a slow and stable growing pattern, a subset of them shows a tendency for local tissue invasiveness and partially increased recurrence rates after surgical excision. Furthermore, heat shock proteins (HSPs) are potentially implicated in ameloblastoma onset and progression. HSPs regulate the folding and refolding of proteins and are induced in response to oxidative stress. They are crucial members of the chaperone intracellular system and are categorized based on their molecular weight (i.e., HSP27, HSP60, HSP70, HSP90). In the current review, we describe HSPs origin and function, focusing on their deregulation mechanisms and impact predominantly on ameloblastomas and also on inflammatory and developmental odontogenic cystic lesions.

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CD34 and Ki-67 Immunoexpression in Periapical Granulomas: Implications for Angiogenesis and Cellular Proliferation

Roi,

Riviș,

Roi

et al. 2024

Diagnostics

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Background/Objectives: The main mechanism of the formation of granulation tissue is the progression of an infection from the tooth to the periapical bone. At this level, the immune system tries to localize and annihilate the microorganism’s injury. Ki-67 is a protein directly associated with the cell proliferation rate, while CD34 is a biomarker involved in angiogenesis, and studies suggest that they both have a positive correlation with the intensity of the local inflammatory infiltrate. This study will determine the immunoexpression of CD34 and Ki-67 in periapical granulomas and assess their impact on the growth and development of this tissue, as well as consider their roles in the proliferative process and aggressiveness of evolution. Methods: In the present study, 35 periapical granulomas obtained after a tooth extraction were included. The specimens were analyzed via histopathology and immunohistochemistry. Results: A positive reaction for the Ki-67 antibody was observed in 32 (86.5%) of the 35 periapical granuloma cases included in our study. We identified the overexpression of Ki-67 and CD34 and further calculated the Ki-67 index to evaluate and correlate the proliferation potential and angiogenesis with regard to the presence of an inflammatory infiltrate. Conclusions: These findings suggest that the persistence of an inflammatory environment directly influences Ki-67 and CD34 expression, sustaining the proliferative capacity of cells and abnormal angiogenesis. This study is the first to evaluate the presence of the CD34+ and Ki-67+ proliferating vessels in periapical granulomas.

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Impact of CD34-dependent Micro Vessel Density on Periapical Odontogenic Cysts

Cited by 3 publications

References 30 publications

Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Heat Shock Proteins’ Expression Profiles in Odontogenic Ameloblastomas and Cysts

CD34 and Ki-67 Immunoexpression in Periapical Granulomas: Implications for Angiogenesis and Cellular Proliferation

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