Impact of Cellulase and Lactic Acid Bacteria Inoculant to Modify Ensiling Characteristics and In Vitro Digestibility of Sweet Corn Stover and Cassava Pulp Silage

Abstract

Improving the productive performance of agricultural residue silage has practical relevance to sustaining livestock production. Sweet corn stover (SCS) and cassava pulp (CSVP) are widespread in the tropics as low-cost feed resources. To efficiently prepare SCS and CSVP silage, the impact of adding Acremonium cellulase (AC), Lactobacillus casei strain TH14 inoculant (TH14), and their combination (AC+TH14) on ensiling characteristics, microbial population, chemical compositions, and in vitro digestibility were tested in comparison to control (no additive). After 60 d of ensiling, the pH value and ammonia nitrogen content of silage were lower (p < 0.05) when AC was used as the additive. Compared with other treatments, TH14 provided abundant lactic acid fermentation in silage. All tested additives increased the crude protein (CP) content and decreased the neutral detergent fiber (NDF) content of SCS silage. In CSVP silage, only AC and AC+TH14 altered the CP and NDF content. In addition, AC and AC+TH14 provided greater in vitro dry matter digestibility and a lower in vitro fiber digestibility. Overall, optimized either AC or TH14 can be recommended as an attractive additive to improve the ensiling characteristics of SCS and CSVP silage. AC significantly modifies the in vitro digestibility of silage.

Characterization of Green Manure Sunn Hemp Crop Silage Prepared with Additives: Aerobic Instability, Nitrogen Value, and In Vitro Rumen Methane Production

“…The reduction of NDF and ADF contents with AC is associated with enzymatic saccharification, releasing fermentable sugars for lactic acid fermentation. The slight delignification of TH14 in GMSH crop silage was consistent with a previous study on cassava pulp silage [41]. Probably, the TH14 strain stimulated the growth of other fiber-decomposing microbes that result in a reduction in fibrous contents and delignification [41].…”

“…The slight delignification of TH14 in GMSH crop silage was consistent with a previous study on cassava pulp silage [41]. Probably, the TH14 strain stimulated the growth of other fiber-decomposing microbes that result in a reduction in fibrous contents and delignification [41].…”

“…The results showed that TH14 increased (p < 0.01) butyric acid and NH 3 N concentrations. This undesirable amino group degradation in GMSH crop silage when adding the TH14 strain contradicts our previous studies using tropical grasses and agricultural by-products [8,10,13,18,41]. This finding probably resulted from an increase in the LBC level, suggesting the addition of TH14 be done with a fermentable sugar source or a new LAB strain to specifically improve leguminous silage production.…”

“…The results suggested that lactic acid fermentation could improve the IVNDFD and IVADFD values obtained with MO and TH14. A lower IVADFD value with AC might result from the condensation of lignocellulose owing to enzymatic saccharification at ensiling [41].…”

Evaluating the effects of cellulolytic enzymes and Lactobacillus bulgaricus on mycotoxins production and the quality of maize silage

“…Therefore, it is necessary to add other microbes that are able to break down lignins during the production of silage. Moreover, Kaewpila et al (2021) found that cellulases or T. harzianum attractive inoculates enhanced the ensiling features of maize silage. In addition, lactic acid production increased when a mixture of enzyme extract and L. bulgaricus was added (Table 3), which demonstrated that the combined enzyme and L. bulgaricus treatment may be more effective compared to the enzyme extract treatment alone; these results were in agreement with a previous study by Li et al (2018).…”