Impact of Chemotherapeutic Stress Depends on The Nature of Breast Cancer Spheroid and Induce Behavioral Plasticity to Resistant Population

Gayan, Sukanya; Teli, Abhishek; Sonawane, Akshay; Dey, Tuli

doi:10.1002/adbi.202300271

Cited by 3 publications

(1 citation statement)

References 94 publications

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“…Simultaneously, dormant cells can re-enter the cell cycle, exhibiting drug resistance and heightened malignancy. Through dormancy, cancer cells gain increased survival capabilities to resist adverse external factors [37,38]. In our study, we demonstrated that mitophagy is associated with the transition of HNSCC into a low-metabolism, low-proliferation dormant state during DDP treatment, endowing the tumor with enhanced drug resistance and CSC characteristics.…”

Section: Discussionmentioning

confidence: 56%

Mitophagy-Mediated Tumor Dormancy Protects Cancer Cells from Chemotherapy

Sun,

Chen,

Liu

et al. 2024

Biomedicines

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Despite obvious tumor shrinkage, relapse after chemotherapy remains a main cause of cancer-related mortality, indicating that a subpopulation of cancer cells acquires chemoresistance and lingers after treatment. However, the mechanism involved in the emergence of chemoresistant cells remains largely unknown. Here, we demonstrate that the degradation of mitochondria via autophagy leads to a dormant state in a subpopulation of cancer cells and confers on them resistance to lethal cisplatin (DDP) exposure. The surviving DDP-resistant cells (hereafter, DRCs) have a lower metabolic rate but a stronger potential malignant potential. In the absence of DDP, these DRCs exhibit an ever-increasing self-renewal ability and heightened tumorigenicity. The combination of chloroquine and DDP exerts potent tumor-suppressive effects. In summary, our findings illuminate the mechanism between mitophagy and tumor dormancy and prove that targeting mitophagy might be a promising approach for overcoming chemoresistance in head and neck squamous cell carcinoma (HNSCC).

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Section: Discussionmentioning

confidence: 56%

Mitophagy-Mediated Tumor Dormancy Protects Cancer Cells from Chemotherapy

Sun,

Chen,

Liu

et al. 2024

Biomedicines

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Breast cancer spheroids prefer activated macrophages as an accomplice: An in vitro study

Teli,

Iyer,

Shanbhag

et al. 2024

Preprint

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Cancer, a heterogeneous disease in nature, often requires help from diverse pro-tumor or tumor-associated- cells, which are recruited and persevered within the stroma. Pro-tumor stromal cells provide the essential support needed for tumor growth, metastasis, and development of drug resistance in due time. Tumor-associated macrophages, one of such cells, are essential to tumor microenvironment and tumor survival. In recent years, TAMs have been identified as potential drug targets and therapeutic agents, which encourages the in-depth characterization of their crosstalk with the tumors. The current study has successfully developed a cost-effective in vitro platform for Chemokine Assisted Recruitment of Macrophages to spheroids mimicking the physiology of TAM recruitment. Firstly, monocytic cell line (u937) were converted into activated naive macrophages (M0) and pro-and anti-inflammatory (M1 and M2) subtypes. Monocytes, M0, M1, and M2 macrophages are characterized extensively. Secondly, the naive and polarized macrophages were subjected to chemokine-dependent recruitment into monotypic and heterotypic breast cancer spheroids. The nature of the recruitment is further investigated by assessing the profile of chemokines and chemokine receptors. Recruited macrophages are also observed to manipulate spheroid behavior in many ways. The recruited macrophages also exhibit an increased level of Siglec-1 (CD169), one of the potential TAM markers. The current platform's potential for application can be extended to understand the recruitment process of other immune/stromal cells to solid tumors. It could be a potential addition to the arrays of in vitro platforms developed to screen the efficiency of cell-based immunotherapeutics in the future.

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Meeting proceedings of the 43rd Indian Association for Cancer Research (IACR)

Malik,

Malaviya

2024

Biology Open

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The 43rd Annual Conference of the Indian Association of Cancer Research (IACR) was held between 19th and 22nd January 2024 at the Indian Institute of Education and Research (IISER), Pune, India. Cancer is the second leading cause of death globally; efforts have been made to understand and treat this deadly disease for several decades. The 43rd IACR, organised by Mayurika Lahiri, Kundan Sengupta, Nagaraj Balasubramanian, Mridula Nambiar, Krishanpal Karmodiya, and Siddhesh Kamat, highlighted recent advances in cancer research, with implications in therapeutics at the forefront of the discussions. The meeting proved to be a promising platform for cancer researchers ranging from graduate and postdoctoral students to subject experts in varied aspects of cancer biology to showcase their research, ideate with their peers, and form collaborations.

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Impact of Chemotherapeutic Stress Depends on The Nature of Breast Cancer Spheroid and Induce Behavioral Plasticity to Resistant Population

Cited by 3 publications

References 94 publications

Mitophagy-Mediated Tumor Dormancy Protects Cancer Cells from Chemotherapy

Mitophagy-Mediated Tumor Dormancy Protects Cancer Cells from Chemotherapy

Breast cancer spheroids prefer activated macrophages as an accomplice: An in vitro study

Meeting proceedings of the 43rd Indian Association for Cancer Research (IACR)

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