Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using 1 H HR-MAS NMR spectroscopy

Maria, Roberta M.; Altei, Wanessa Fernanda; Selistre-de-Araújo, Heloísa Sobreiro; Colnago, Luiz Alberto

doi:10.1016/j.jpba.2017.08.038

Cited by 40 publications

(39 citation statements)

References 22 publications

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“…As alterations in cellular metabolism may contribute to the development of a malignant phenotype and cell resistance, MRS applications in tumor response assessment is particularly valuable. In a recent study, Maria et al investigated 1 H HR‐MAS NMR spectroscopy for the characterization of the metabolic profile of metastatic mammary adenocarcinoma breast cancer MDA‐MB‐231 cells either untreated (control) or treated with tamoxifen, cisplatin, and doxorubicin. NMR spectra showed that phosphocholine (a biomarker of breast cancer malignant transformation) signals were stronger in control than in treated cells, but significantly decreased upon treatment with tamoxifen/cisplatin.…”

Section: Clinical Applicationsmentioning

confidence: 99%

Proton MR spectroscopy in the breast: Technical innovations and clinical applications

Fardanesh

Marino

Avendaño

et al. 2019

Magnetic Resonance Imaging

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Proton magnetic resonance spectroscopy (MRS) is a promising noninvasive diagnostic technique for investigation of breast cancer metabolism. Spectroscopic imaging data may be obtained following contrast‐enhanced MRI by applying the point‐resolved spectroscopy sequence (PRESS) or the stimulated echo acquisition mode (STEAM) sequence from the MR voxel encompassing the breast lesion. Total choline signal (tCho) measured in vivo using either a qualitative or quantitative approach has been used as a diagnostic test in the workup of malignant breast lesions. In addition to tCho metabolites, other relevant metabolites, including multiple lipids, can be detected and monitored. MRS has been heavily investigated as an adjunct to morphologic and dynamic MRI to improve diagnostic accuracy in breast cancer, obviating unnecessary benign biopsies. Besides its use in the staging of breast cancer, other promising applications have been recently investigated, including the assessment of treatment response and therapy monitoring. This review provides guidance on spectroscopic acquisition and quantification methods and highlights current and evolving clinical applications of proton MRS. Level of Evidence 5 Technical Efficacy: Stage 5 J. Magn. Reson. Imaging 2019.

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Section: Clinical Applicationsmentioning

confidence: 99%

Proton MR spectroscopy in the breast: Technical innovations and clinical applications

Fardanesh

Marino

Avendaño

et al. 2019

Magnetic Resonance Imaging

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“…10 The spectra of MCF-7 and MDA-MB-231 cells have been previously assigned elsewhere using 1D NMR database and 2D NMR experiments. 20,21 The major components of these cells were lactate quartet at The non-overlapped peak of each metabolite was used to calculate its relative intensity (in triplicate) in relation to the total area of each normalized spectrum. The intensities of some signals were significantly different in both control BC cell lines.…”

Section: Resultsmentioning

confidence: 99%

“…Reduced phosphocholine levels were also observed in these BC cells upon treatment with commercial chemotherapy drugs, such as tamoxifen, cisplatin, and doxorubicin. 20,21 However, CLA showed minor influence on other metabolites when contrasted with commercial drugs, indicating that CLA may cause fewer side effects even when used at much higher concentrations.…”

Section: Discussionmentioning

confidence: 99%

Effect of cis-9, trans-11 Conjugated Linoleic Acid (CLA) on the Metabolism Profile of Breast Cancer Cells Determined by 1 H HR-MAS NMR Spectroscopy

Maria

Altei

Valadares

et al. 2018

J. Braz. Chem. Soc.

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Conjugated linoleic acid (CLA), a fatty acid found in ruminant food products, has been associated with anticarcinogenic activity. However, its effect on cancer metabolism is unclear. In this paper we evaluated the effects of cis-9, trans-11 CLA on the metabolic profile of MCF-7 and MDA-MB-231 breast cancer cells using high-resolution magic angle spinning (HR-MAS) nuclear magnetic resonance (NMR) spectroscopy. The NMR spectra showed that phosphocholine level, a cancer malignance biomarker, was reduced in both cells treated with CLA, but the reduction was more pronounced in MCF-7 cells. The NMR spectra also showed that CLA has opposite effect on MCF-7 and MDA-MB-231 acetone metabolism. Acetone signal has been observed in the spectra of MDA-MB-231 control cells, but not in the spectra of the cells treated with 50 and 100 µM CLA. Conversely, the acetone signal is very small or not observed in the NMR spectra of MCF-7 control cells and in cells treated with 50 µM of CLA, but is very strong in the spectra of the cells treated with 100 µM of CLA. Therefore, this CLA concentration is causing a ketosis in MCF-7 cells by inducing the use of fatty acids as an energy source or by reducing acetone catabolism. These results indicate that CLA interfere in the metabolism of both cells. However, the strongest effect has been observed on the metabolism of MCF-7 cells cultivated in the presence of 100 µM CLA. Therefore, CLA could be a potential anticarcinogenic drug, especially for cells with positive estrogen receptor, such as MCF-7.

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“…All patients in the current study suffered from invasive ductal carcinoma, and they had an elevated level of tCho before chemotherapy. Elevated tCho concentration was previously reported in many studies in malignant neoplasms [19][20][21]. The composite Cho resonance reflects the abnormal cellular proliferation and is considered as a biomarker of the rapid growth rate of malignant tumours that can be assessed in association with tumour size to achieve better diagnostic accuracy [22,23].…”

Section: Discussionmentioning

confidence: 98%

The additive role of 1H-magnetic resonance spectroscopic imaging to ensure pathological complete response after neoadjuvant chemotherapy in breast cancer patients

et al. 2019

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Purpose:To assess the role of 1 H-magnetic resonance spectroscopy ( 1 H-MRS) in the confirmation of pathological complete response after neoadjuvant chemotherapy in breast cancer. Material and methods:Forty-seven cases (53.72 ± 8.53 years) were evaluated using magnetic resonance imaging (MRI) and 1 H-MRS with choline (Cho) signal-to-noise ratio (SNR) measured followed by histopathology and ROC analyses. Results:Twelve patients had complete response, and 35 patients had residual disease. Mean age was 53.72 ± 8.53 years.The mean tumour size before neoadjuvant chemotherapy (NAC) was 4.21 ± 0.99 cm and after NAC was 0.9 ± 0.44 cm. Positive total choline signal (tCho) was detected in all cases. The mean Cho SNR before NAC was 9.53 ± 1.7 and after NAC was 2.53 ± 1.3. The Cho SNR cut-off point differentiating between pathologic complete response (pCR) and the non pCR was 1.95. Dynamic MRI showed 83.3% sensitivity, 65.7% specificity, 45.5% positive predictive value, 92.0% negative predictive value, and 70.2% diagnostic accuracy. Combined evaluation done by using the dynamic MRI and 1 H-MRS showed 91.5% diagnostic accuracy with 75.0% sensitivity, 97.1% specificity, 75% positive predictive value, and 91.9% negative predictive value. ROC curves of Cho SNR showed statistically significant differences between non pCR and pCR with AUC was 0.955, 82.9% sensitivity, 91.7% specificity, 96.7% positive predictive value, 64.7% negative predictive value, and 85.11% diagnostic accuracy.Conclusions: 1 H-MRS improves the diagnostic accuracy in the prediction of the pCR after NAC.

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Impact of chemotherapy on metabolic reprogramming: Characterization of the metabolic profile of breast cancer MDA-MB-231 cells using 1 H HR-MAS NMR spectroscopy

Cited by 40 publications

References 22 publications

Proton MR spectroscopy in the breast: Technical innovations and clinical applications

Proton MR spectroscopy in the breast: Technical innovations and clinical applications

Effect of cis-9, trans-11 Conjugated Linoleic Acid (CLA) on the Metabolism Profile of Breast Cancer Cells Determined by 1 H HR-MAS NMR Spectroscopy

The additive role of 1H-magnetic resonance spectroscopic imaging to ensure pathological complete response after neoadjuvant chemotherapy in breast cancer patients

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