Impact of Chromium Oxide Nanoparticles on Growth and Biofilm Formation of Persistence Klebsiella pneumoniae Isolates

Marjani, Mohammed Al; Aziz, Sarah Naji; Rheima, Ahmed Mahdi; Abbas, Zainab Sabri

doi:10.5101/nbe.v13i3.p321-327

Cited by 16 publications

(12 citation statements)

References 25 publications

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“…The Vitek-2 system was used to identify and cultivate all of the isolates on Luria Bertani (LB, Difco Laboratories) agar plates or in LB broth at a temperature of 37 °C. Based on our previous study, we determined the Persister cell assay [20].…”

Section: Bacterial Isolates and Growth Conditionsmentioning

confidence: 99%

DBD plasma as a practical approach to sterilization of dental instruments

Mohammed,

Al -Marjani

2024

Phys. Scr.

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Microbe control and decontamination are necessary for medical equipment that will be used again. Several wet and dry sterilizer technologies, like as autoclaves, ovens, UV systems, and chemical agents, have been widely used to eliminate the possibility of cross-infection between contaminated dental instruments or materials and dental patients. The development of a sterilizer device capable of sterilizing dental equipment through non-toxic materials has been shown to significantly decrease the overall procedure time compared to conventional approaches. Therefore, an investigation was conducted to assess the efficacy of a dielectric barrier discharge (DBD) plasma device in sterilizing dental instruments under atmospheric pressure conditions. Also, due to Reactive Oxygen and Nitrogen Species (RONS) existence at low temperatures, researchers have been examining the biological effects of cold plasmas for medical applications with promising results. The dental Tweezer, one of the dental tools, was chosen to perform the sterilization process using the DBD plasma device. The Dental Tweezers specimens were contaminated with Escherichia coli and Staphylococcus aureus bacteria, and then the DBD plasma device was used to sterilize them for 1, 2, 3, and 4 minutes, respectively, to figure out the best time to do this. The DBD plasma device successfully killed E. coli and S. aureus. Based on the findings derived from our present investigation, the cold plasma device has impressively proven its efficiency in sterilization.

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Section: Bacterial Isolates and Growth Conditionsmentioning

confidence: 99%

DBD plasma as a practical approach to sterilization of dental instruments

Mohammed,

Al -Marjani

2024

Phys. Scr.

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“…bacterial persistence, we have the potential to reduce bacterial resistance to antibiotics and drugs, ultimately leading to improved outcomes in the treatment of chronic infections [24]. In this study, we examined the impact of ZnO and TiO 2 NPs on the biofilm formation and persister cells in K. pneumoniae.…”

Section: The Expression Level Of Mrkh Fimh Mazf and Luxs Genesmentioning

confidence: 99%

Investigating the effects of zinc oxide and titanium dioxide nanoparticles on the formation of biofilm and persister cells in Klebsiella pneumoniae

Pourmehdiabadi,

Nobakht,

Hajjam Balajorshari

et al. 2023

J Basic Microbiol

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The biofilm formation in klebsiella pneumoniae isolates poses a significant problem as it can result in treatment failure and the development of chronic infections. These biofilms act as protective barriers, rendering the bacteria resistant to antibiotics. Additionally, persister cells, which make up a small fraction of the bacterial population, have the ability to enter a dormant state after treatment with high doses of antibiotics. These persister cells play a crucial role in the high level of biofilm‐mediated tolerance to antibiotics. The present study aimed to investigate the impact of Zinc oxide (ZnO) and titanium dioxide (TiO2) nanoparticles on the formation of biofilm and persister cells in K. pneumoniae. The minimum inhibitory concentration (MIC) of colistin in K. pneumoniae ATCC 13883 was determined using the microdilution method. The formation of persister cells was evaluated by introducing sub‐MIC of colistin. Subsequently, the MIC of ZnO NPs and TiO2 NPs in these persister cells was assessed using the microdilution method. Furthermore, the effects of nanoparticles on the expression levels of biofilm‐associated genes were analyzed using real‐time polymer chain reaction (PCR). The MIC values for colistin, ZnO, and TiO2 were determined at 2, 12.5, and 6.25 μg/mL, respectively. In the presence of nanoparticles, biofilm formation decreased. Real‐time PCR results showed the messenger RNA (mRNA) level of mrkH and fimH were decreased and the expression of luxS and mazF were increased. Biofilm formation of K. pneumoniae ATCC 1383 was inhibited in response to nanoparticles. According to the results of the present study use of nanoparticles may help control multidrug‐resistant (MDR) infections in hospitalized patients.

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“…Many studies have recently shown the synergistic effect of silver nanoparticles and graphene nano-sheets against microorganisms, including MDR pathogenic bacteria of K. pneumoniae, P. aeruginosa, E. coli, and A. baumannii [126][127][128]. Another recent study [129], showed that chromium oxide nanoparticles (Cr 2 O 3 -NPs) have important applications in different fields due to their physical and chemical properties that depending on their shape and size. Chromium (Cr) is a transitional metal that can exist in different oxidation states.…”

Section: Control Of Heteroresistance and Persistent Bacteriamentioning

confidence: 99%

Overview of heteroresistance, persistence and optimized strategies to control them

Marjani,

Mohammed,

Al-Kadmy

et al. 2022

Reviews and Research in Medical Microbiology

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Antibiotic-resistant bacteria have become much more prevalent, posing a significant concern to universal care. In basic concepts, the phenomenon of heteroresistance is typified as antibiotic resistance displayed by a subgroup of the entire bacterial community which is typically thought to be sensitive to these antibiotics based on standard in-vitro sensitivity assays. Although this small subgroup is temporarily dormant nondividing state displays enhanced resistance to broad antibiotics ranges. Persistence is a subpopulation of a sensitive bacterial isolate that can tolerate the bactericidal antibiotics because of its dormant physiological state. Until recent times, the occurrence of heteroresistance and persistence phenomena are often misunderstood and perhaps neglected. Here, we review the outline literature about bacterial heteroresistance, their detection methods, their associated mechanisms, the objectives of some bacterial species to develop heteroresistance. Bacterial persistence, some of their features, their main mechanisms, and their clinical importance are also described. Furthermore, this study provides optimized strategies to combat both phenomena.

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Impact of Chromium Oxide Nanoparticles on Growth and Biofilm Formation of Persistence Klebsiella pneumoniae Isolates

Cited by 16 publications

References 25 publications

DBD plasma as a practical approach to sterilization of dental instruments

DBD plasma as a practical approach to sterilization of dental instruments

Investigating the effects of zinc oxide and titanium dioxide nanoparticles on the formation of biofilm and persister cells in Klebsiella pneumoniae

Overview of heteroresistance, persistence and optimized strategies to control them

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