Impact of colchicine application during callus induction and shoot regeneration on micropropagation and polyploidisation rates in two Miscanthus species

Głowacka, Katarzyna; Jeżowski, S.; Kaczmarek, Z.

doi:10.1007/s11627-010-9282-y

Cited by 11 publications

(2 citation statements)

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“…Therefore, synthetic polyploidisation has been utilised for breeding for many years. Recently, in vitro polyploidisation techniques have been developed for Salix viminalis (Wojciechowicz 2009), Humulus lupulus (TrojakGoluch and Skomra 2013), Miscanthus (Głowacka et al 2010), tulip (Podwyszyńska 2011), Centella asiatica (Kaensaksiri et al 2011), Pyrus communis (Sun et al 2011), and Gerbera jamesonii (Gantait et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

et al. 2014

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Tetraploids of daylily have taken a leading position among the daylily cultivars due to desirable traits such as vigorous growth and flowers with more intense colour. In our previous studies, several tetraploids of daylily cultivars 'Blink of an Eye' and 'Berlin Multi' were obtained using in vitro techniques with different antimitotic agents (colchicine, oryzalin, trifluralin, and amiprophos methyl). The purposes of this study were to evaluate changes in daylily tetraploids in relation to their diploid counterparts and to assess variation among diploids and tetraploids derived from genetically homogenous plant material treated with antimitotic agents. In the first year of the ex vitro cultivation, growth of tetraploids was poorer in comparison with diploids, but in the second year, tetraploid growth was much more vigorous. Compared to diploids, in tetraploids of both cultivars percentage of flowering plants was lower, flowering was delayed by 8 days and 1 month in 'Blink of an Eye' and 'Berlin Multi', respectively, and bud number per scape was lower by approximately 20 and 40 %, respectively. Tetraploid leaves and flowers of both cultivars were significantly larger, chlorophyll concentration index was higher by approximately 40 %, and stomata were longer by 35 %. In 'Berlin Multi' tetraploids, inflorescence stems were shorter by 20 %. In 'Blink of an Eye', variation in flower colour tone and shape and stamen malformation rate was detected both within the unconverted diploids and tetraploids but was more evident in tetraploids. In both cultivars, variation was also found in the nuclear DNA content, which ranged in diploids and tetraploids, respectively, 8.02-8.53 and 16.01-17.13 pg in 'Berlin Multi ' and 8.28-8.71 and 15.93-17.36 pg in 'Blink of an Eye'. Since the variation, while less evident, also occurred in the diploids (regenerated from the antimitotic treated material), we suppose that these variations could be due to antimitotic agents that can induce not only chromosome doubling but also chromosomal and gene mutations. The extent and character of these changes can be related to parental genotype and/or antimitotic agent. Further research is required at the cytological and molecular level to explain the character of changes, epigenetic and/or genetic.

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Section: Introductionmentioning

confidence: 99%

Phenotypic and genome size changes (variation) in synthetic tetraploids of daylily (Hemerocallis) in relation to their diploid counterparts

et al. 2014

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“…Chromosome doubling has been used to generate artificial polyploids in Miscanthus and has potential to introduce new genetic diversity into breeding programmes especially for M. × giganteus types by manipulating the ploidy of the parental species, restoring fertility or disrupting the self-incompatibility system (Petersen et al , 2002; Głowacka et al , 2009, 2010a, b; Yu et al, 2009). Another stimulus for artificial polyploid formation has been the desire to generate novel sterile genotypes that lower the risk of invasiveness following introduction as a crop (Petersen et al , 2003; Barney and Ditomaso 2008; Jørgensen, 2011).…”

Section: Chromosome Variationmentioning

confidence: 99%

Miscanthus: a case study for the utilization of natural genetic variation

Hodkinson

Klaas

Jones

et al. 2014

Plant Genet. Resour.

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Cultivars of Miscanthus used as bioenergy crops or tested in trials are largely clonally propagated, wild sourced genotypes or clonally propagated F1 hybrids. One of the most productive taxa is the sterile triploid M. £ giganteus. Little domestication or breeding has been undertaken and there is huge potential to utilize the extensive genetic resources of the genus for crop improvement. The challenge is to generate new highly adapted genotypes suitable for a range of environments. Production on marginal land, not used for food crops, is particularly desirable, but presents many barriers to crop breeders, as these are largely unproductive and/or stressful environments. This article outlines progress made in characterizing natural genetic variation in Miscanthus including next-generation single-nucleotide polymorphism genotyping, quantitative trait locus analysis and association mapping. It also explains how this knowledge is being used to develop novel genotypes suited for growth in a broad range of agricultural and marginal lands by defining breeding pools, generating novel crosses, manipulating polyploidy and applying genomic selection approaches.

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