Impact of conditions of cultivation and adsorption on antimicrobial activity of marine bacteria

Ivanova, Elena P.; Nicolau, Dan V.; Yumoto, Noboru; Taguchi, Takahisa; Okamoto, Kyoichi; Tatsu, Yoshiro; Yoshikawa, Shuji

doi:10.1007/s002270050275

Cited by 46 publications

(36 citation statements)

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“…Studies in TDA-producing Phaeobacter strains have shown biosynthesis occurring at measureable levels in shaking and stagnant broth cultures and on agar plates (22,44). This is the first report of antimicrobial production by a Roseobacter in response to surface colonization; however, several studies with unclassified marine isolates have shown similar results (31,56,61). Furthermore, particle-associated representatives of the Roseobacter clade have been previously demonstrated to be Ͼ10 times more likely to produce antimicrobial compounds than free-living members (37).…”

Section: Resultsmentioning

confidence: 72%

Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I

Cude

Mooney

Tavanaei

et al. 2012

Appl Environ Microbiol

132

134

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ABSTRACTMembers of theRoseobacterlineage of marine bacteria are prolific surface colonizers in marine coastal environments, and antimicrobial secondary metabolite production has been hypothesized to provide a competitive advantage to colonizing roseobacters. Here, we report that the roseobacterPhaeobactersp. strain Y4I produces the blue pigment indigoidine via a nonribosomal peptide synthase (NRPS)-based biosynthetic pathway encoded by a novel series of genetically linked genes:igiBCDFE. A Tn5-based random mutagenesis library of Y4I showed a perfect correlation between indigoidine production by thePhaeobacterstrain and inhibition ofVibrio fischerion agar plates, revealing a previously unrecognized bioactivity of this molecule. In addition, igiD null mutants (igiD encoding the indigoidine NRPS) were more resistant to hydrogen peroxide, less motile, and faster to colonize an artificial surface than the wild-type strain. Collectively, these data provide evidence for pleiotropic effects of indigoidine production in this strain. Gene expression assays support phenotypic observations and demonstrate thatigiDgene expression is upregulated during growth on surfaces. Furthermore, competitive cocultures ofV. fischeriand Y4I show that the production of indigoidine by Y4I significantly inhibits colonization ofV. fischerion surfaces. This study is the first to characterize a secondary metabolite produced by an NRPS in roseobacters.

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Section: Resultsmentioning

confidence: 72%

Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I

Cude

Mooney

Tavanaei

et al. 2012

Appl Environ Microbiol

132

134

View full text Add to dashboard Cite

show abstract

“…In a study carried out on 238 bacteria associated with two Mediterranean sponges, Hentschel et al [6] reported a detection rate of 11.3%; Nair and Simidu [3] found that the 13.3% of the free-living bacteria tested inhibited the growth of S. aureus; Ivanova et al [4] screened 491 bacteria isolated from different marine sources, detecting the 26% of active isolates; finally, Zheng et al [9] selected eight among the twenty-nine strains (28%) they tested due to their ability to inhibit at least one of the target microorganisms. Moreover, in the present study the percentage of marine bacteria showing inhibitory activity was higher than that recorded by O'Brien et al However, it could be noted that the differences among the detection rates reported in literature strongly depend on both the isolation and assay procedures (including the organisms used as a target and the media employed), as well as on the sources of bacterial isolates.…”

Section: Discussionmentioning

confidence: 99%

“…Experiments were performed on both MA and the solid medium proposed by Ivanova et al [4], containing (w/v): 0.2% Bactopeptone, 0.2% casein hydrolysate, 0.2% yeast extract, 0.1% glucose, 0.02% KH 2 PO 4 , 0.005% MgSO 4 × 7 H 2 O, 0.1% CaCl 2 , 0.01% KBr and 1.5% Bacto-agar. The latter medium was prepared in a mixture of 75% (v/v) natural seawater and 25% (v/v) distilled water (pH 7.8).…”

Section: Screening For Inhibitory Activity Against Indicator Organismsmentioning

confidence: 99%

Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms

Giudice

Bruni

Michaud

2007

J. Basic Microbiol.

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Five-hundreds and eighty bacterial strains, isolated from various Antarctic marine sources and locations, were screened for antimicrobial activity against terrestrial microorganisms. Twenty-two Antarctic isolates (3.8%), mainly retrieved from the water column at Terra Nova Bay (Ross Sea), expressed antagonistic activity against one to three indicator organisms. Escherichia coli and Proteus mirabilis resulted as the more susceptible, followed by Micrococcus luteus and Bacillus subtilis. None of the isolates inhibited the growth of Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella enterica and the eukaryotic fungus Candida albicans. Active Antarctic isolates, identified by 16S rDNA sequencing and phenotypically characterized by classical methods, were phylogenetically affiliated to the Actinobacteria (16 strains) and the gamma-Proteobacteria (6 strains). Inhibition patterns, as well as phenotypic characteristics, highly vary for different isolates, even though they were affiliated to the same genus or closely related to the identical microorganism retrieved from the database, suggesting that these features were more likely strain-rather than species-specific.Results obtained from the present study confirm previous observations and highlight the potentiality of Antarctic marine bacteria as novel source of antibacterial substances.

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“…Polymeric surfaces, in particular photosensitive polymers with light-tunable properties [25], of which PtBMA is a member [26], have previously been used to probe the surface-induced metabolic change of surface-anchored marine bacteria [27]. Compared to other polymers, PtBMA has certain experimental advantages, i.e.…”

Section: Resultsmentioning

confidence: 99%

Detection of coccoid forms of Sulfitobacter mediterraneus using atomic force microscopy

Ivanova¹

2002

FEMS Microbiology Letters

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The adhesion of the marine K-Proteobacteria Sulfitobacter pontiacus, Sulfitobacter mediterraneus, Sulfitobacter brevis, and Staleya guttiformis to a poly(tert-butyl methacrylate) (PtBMA) polymeric surface generates unusual cell morphological peculiarities following attachment. While the type strains S. pontiacus and S. brevis failed to attach to PtBMA, the vegetative cells of type strain S. mediterraneus underwent morphological conversion into coccoid forms during the attachment over an incubation period of 24^72 h. Type strain St. guttiformis cells formed a multilayered biofilm on the PtBMA surface, presumably facilitated by bacterial production of extracellular polysaccharides. The attachment behavior and fine structure of these coccoid forms have been described using atomic force microscopy. The impact of polymeric surfaces of defined hydrophobicity on the formation of coccoid bodies is discussed. ß

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Impact of conditions of cultivation and adsorption on antimicrobial activity of marine bacteria

Cited by 46 publications

References 0 publications

Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I

Production of the Antimicrobial Secondary Metabolite Indigoidine Contributes to Competitive Surface Colonization by the Marine Roseobacter Phaeobacter sp. Strain Y4I

Characterization of Antarctic psychrotrophic bacteria with antibacterial activities against terrestrial microorganisms

Detection of coccoid forms of Sulfitobacter mediterraneus using atomic force microscopy

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