Impact of Conventional and Non-Conventional Extenders on Rams Semen Quality During Storage at 5ºC

Abstract

The aim of the present study was to define the effect of different extender components (as conventional and nonconventional extenders) on rams' semen quality during storage at 5ºC for up to 6 days. Fertility rates of the extended ram spermatozoa stored at 5ºC for up one day were also studied. Four Rahmani rams were used to collect pooled ejaculates by artificial vagina. Semen was collected, evaluated and extended with five extenders included conventional and non-conventional extender ingredients. The conventional extenders were sodium citrate (E1), Tris (E2) and skimmed milk powder (E3)-based glucose-egg yolk. While, non-conventional extenders were salt of sodium chloride 0.9% (E4) and saline solution 0.9% intravenous infusion (E5) based glucose-egg yolk. The final extension rate was 1semen : 6 extender. The extended semen with different extenders media (E1, E2, E3, E4 and E5) were cooled to 5ºC and stored at this temperature for 6 days. After storage time (0, 1, 2, 3, 4, 5 and 6 days), the percentage of sperm motility (SPM), recovery sperm motility (RSM) and positive osmotic resistant (POR) were recorded. Also, fertility rate was carried out with liquid semen stored at 5ºC for up to one day using the best conventional and non-conventional extenders. Thus, twenty-two ewes were used to investigate fertility rate. Ewes were divided into two groups (11/group), the1 st and 2 nd groups were artificially inseminated with conventional (E2) or non-conventional (E5) extenders which obtained better semen quality than other extenders, respectively. The results indicated that, the cooling extended ram semen with each of E1, E2 or E5 extenders was significantly (P<0.05) higher the percentage of SPM, RSM and POR than those extended with E3 and E4 extenders during storage at 5ºC for up to 6 days. However, the cooling ram semen with E2 and E5 showed non-significantly parameters during cooled at 5ºC till 6 days. The statistical analysis of the data revealed a significant positive correlation between extender types and SPM, RSM and POR of ram spermatozoa during storage at 5ºC until 6 days. Surprisingly, fertility rate of ewes artificially inseminated with ram semen preserved at 5ºC for one day of storage performed to assess higher conception rate (84.62%) with E5 (as non-conventional extender) than conception rate (78.57%) with E2 (as conventional extender). Moreover, the superior litter size recorded 1.00 with ram semen extended with E5 compared to 0.86 with E2 extenders. Also, sex ratio of born lambs displayed female lambs 66.67 and 33.33% of male lambs with semen extended with E2 extender. Ram semen extended with E5 achieved female lambs 69.23% and male lambs 30.77%. It is therefore concluded that saline solution (0.9%) intravenous infusion (E5) based glucose-egg yolk intended to keep goodness fertility rate and litter size after AI of cooled semen at 5ºC.

Effect of Propolis Ethanolic Extract Supplementation to Ram Semen Extenders on Sperm Characteristics, Lipid Peroxidation and some Enzymatic Activities in Seminal Plasma in Chilled Semen

“…Advancement of storage time for up to 48 hours in extender types can depressed significantly (P<0.05) the percentage of advanced sperm motility. These results are similar to that reported in stallion (Santos, 2014), bull (El-Sisy et al, 2016), rabbit (El-Seadawy et al, 2017) and ram (Khalifa and Khalil, 2016). Decreasing progressive motility attainted to gradual depletion of nutrients (Gibb and Aitken, 2016), enhance productive of free radical (Acharya et al, 2016) and inhibit of fructolysis and respiration spermatozoa (Gündoğan et al, 2003).…”

Anti-oxidative influence of butylated hydroxytoluene on chilled semen of Red Sokoto bucks and consequential conception rates in does

“…This further corroborates the report of Mahiddine & Kim (2021), that post-thaw parameters of cryopreserved sperm are poorer than those of fresh sperm. The sperm motility recorded in this study are similar to that reported in rams (Khalifa & Khalil 2016). The decreasing sperm motility observed was attributed to depletion of nutrients (Gibb & Aitken 2016), which inhibit fructolysis and respiration of sperm cells (Gündoğan et al, 2003).…”

The possibility of using citric phosphate dextrose in chilling ram semen instead of egg yolk and soybean lecithin to improve fertility

“…Our findings postulated that EGD, SLD and CPDA extenders indicated better semen characteristics such as motility, live, normal and intact acrosome than CPD extender maybe had a positive effect on reproductive performance. Confirmation by Khalifa and Khalil (2016) who find out that Tris‐based extender with egg yolk and soybean lecithin has a beneficial effect on chilling characteristics of ram semen extender which lead to the best conception and fertility rates. On the other hand, Mostafa et al.…”

“…Our findings postulated that EGD, SLD and CPDA extenders indicated better semen characteristics such as motility, live, normal and intact acrosome than CPD extender maybe had a positive effect on reproductive performance. Confirmation by Khalifa and Khalil (2016) who find out that Tris-based extender with egg yolk and soybean lecithin has a beneficial effect on chilling characteristics of ram semen extender which lead to the best conception It has found been that the best conception rate and fertility rate in EGD and SLD may be attributed to protein in egg yolk (Anzar, Rajapaksha, & Boswall, 2019) and soybean lecithin (Baruah et al, 2019). The lowest conception rate and fertility rate in semen diluted with CPD (without antibiotic) extender could also be due to contamination of handling semen during collection and preservation (Khalifa, Mohamed, & Abdel-Hafez, 2016, 2016b.…”

“…Hence, the materials of cold shocks such as egg yolk and soybean lecithin-based extenders have been applied during storage. For instance, extenders of ram (Khalifa & Khalil, 2016), billy goats (Khalifa, 2015), bull (Gamal, El-Nattat, El-Sheshtawy, & El-Maaty, 2016) and buck (Baruah et al, 2019) had positive sperm parameters in egg yolk-based extender. As well as, the soybean lecithin-based extender is used to protect cold shock of ram Khalifa, Mohamed, et al, 2016), billy goats (Narwade, Mohanty, Bhakat, & Rahim, 2017) and buck (Baruah et al, 2019).…”