Impact of culture media and sampling methods on<i>Staphylococcus aureus</i>aerosols

Chang, Ching‐Wen; Wang, L.-J.

doi:10.1111/ina.12162

Cited by 12 publications

(3 citation statements)

References 44 publications

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“…The capture of S. aureus from exhaled breath with the BESS device but not filters could be attributed either to low recovery efficiencies for S. aureus from the filters, as reported elsewhere (Chang and Wang, 2015); or to sources of error such as contamination during sample handling or collection. S. aureus is a known commensal organism, thus prone to spread through contact easily.…”

Section: Discrepanciesmentioning

confidence: 69%

Electrostatic Sampling of Patient Breath for Pathogen Detection: A Pilot Study

et al. 2020

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Collecting samples for diagnosis of respiratory infections can be invasive and highly uncomfortable for patients; with sampling techniques ranging from nasal or throat swabs to bronchoalveolar lavage. In this work, we explore the electrostatic capture of exhaled pathogens as a non-invasive sampling method for rapid diagnosis in a primary care setting. A pilot study at primary care centers in Belgium enrolled 20 patients presenting with flu-like symptoms whose diagnosis was determined by nasopharyngeal (NP) swabs. We collected exhaled aerosol particles from infected patients using a breath electrostatic sampler (BESS) and using filters, following an extremely light procedure consisting of five normal exhales. All samples were analyzed using a commercial multiplex q-RT-PCR panel assay. Staphylococcus aureus (S. aureus) was detected from BESS samples of seven patients; three of whom were in agreement with their corresponding diagnoses. The BESS method was negative for exhaled viruses. We detected viruses, but no bacteria, with the filters, but the results showed no correlation with the corresponding diagnosis. Our results indicate that electrostatic sampling of exhaled breath is a technique and approach potentially suited for the primary care setting, where it might constitute a helpful diagnostic device.

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Section: Discrepanciesmentioning

confidence: 69%

Electrostatic Sampling of Patient Breath for Pathogen Detection: A Pilot Study

et al. 2020

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“…Moreover, previous studies in dental offices sampled only the air of the consulting rooms which are largely contaminated by bioaerosols due to the widespread use of high-speed dental turbines, hand pieces, and mechanical scalers [41]; however, this study sampled the air in waiting and sterilization rooms. Finally, the use of a cyclonic liquid air sampler (Coriolis®µ) in this study could also partly explain this difference; other studies have used impactors to sample the air [46,47].…”

Section: Microorganisms: Bacteria and Fungimentioning

confidence: 98%

Indoor Air Quality in Healthcare and Care Facilities: Chemical Pollutants and Microbiological Contaminants

et al. 2021

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The indoor air quality of healthcare and care facilities is poorly studied. The aim of this study was to qualitatively and quantitatively describe the chemical pollution and the microbiological contaminations of the indoor environment of these facilities. Methods: A wide range of chemical compounds (39 volatile and 13 semi-volatile organic compounds, carbon dioxide, fine particulate matter) and microorganisms (fungi and bacteria) were studied. Sampling campaigns were conducted in two French cities in summer 2018 and winter 2019 in six private healthcare facilities (general practitioner’s offices, dental offices, pharmacies) and four care facilities (nursing homes). Results: The highest median concentrations of chemical compounds (μg/m3) were measured for alcohols (ethanol: 378.9 and isopropanol: 23.6), ketones (acetone: 18.8), aldehydes (formaldehyde: 11.4 and acetaldehyde: 6.5) and terpenes (limonene: 4.3). The median concentration of PM2.5 was 9.0 µg/m3. The main bacteria of these indoor environments were Staphylococcus, Micrococcus and Bacillus genera, with median bacterial concentrations in the indoor air of 14 cfu/m3. The two major fungal genera were Cladosporium and Penicillium, with median fungal concentrations of 7 cfu/m3. Conclusions: Indoor air in healthcare and care facilities contains a complex mixture of many pollutants found in higher concentrations compared to the indoor air in French hospitals in a previous study.

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“…[9][10][11] A myriad of air samplers have been developed including adsorption tubes, passive badges, impactors, cyclones, and impingers. [12][13][14][15][16][17][18][19][20][21] Detailed reviews of the most popular conventional sampling techniques can be found in the works of Marple, 22 Henningson et al, 23 Kulkarni et al, 24 Chang et al, 25,26 and Ghosh et al 27 The impingement technique is one of the typical approaches in which airborne particles are collected in an extraction liquid. So far, different types of impingers have been designed; however, only a few of them, such as the All Glass Impinger 30 (Ace Glass Inc.) 10,25,26,[28][29][30][31][32][33][34] and the BioSampler (SKC Inc.), 10,25,26,[35][36][37][38][39][40][41] have been success-ful enough to be recognized as reference particle samplers in various applications.…”

Section: Introductionmentioning

confidence: 99%

A microfluidics-based on-chip impinger for airborne particle collection

et al. 2016

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Capturing airborne particles from air into a liquid is a critical process for the development of many sensors and analytical systems. A miniaturized airborne particle sampling device (microimpinger) has been developed in this research. The microimpinger relies on a controlled bubble generation process produced by driving air through microchannel arrays. The particles confined in the microscale bubbles are captured in the sampling liquid while the bubbles form, are released and travel in a millimetre-scale sealed liquid reservoir. The microchannel arrays in the impinger are fabricated using a soft-lithography method with polydimethylsiloxane (PDMS) as the structural material. To prevent air leakage at the connections, a PDMS-only sealing technique is successfully developed. The hydrophobicity of the microchannel surface is found to be critical for generating continuous and stable bubbles in the bubbling process. A Teflon layer is coated on the walls of a microchannel array by vapor deposition which effectively increases the hydrophobicity of the PDMS. The collection efficiency of the microimpinger is measured by counting different sizes of fluorescent polystyrene latex particles on polycarbonate membrane filters. Collection efficiencies above 90% are achieved. Furthermore, the particle capturing mechanisms during the injection, formation and rise of a single microbubble are investigated by a computational fluid dynamics (CFD) model. The Navier-Stokes equations are solved along with the use of the volume-of-fluid (VOF) method to capture the bubble deformations and the particles are tracked using a Lagrangian equation of motion. The model is also employed to study the effect of bubble size on the collection efficiency of the microimpinger.

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Impact of culture media and sampling methods onStaphylococcus aureusaerosols

Cited by 12 publications

References 44 publications

Electrostatic Sampling of Patient Breath for Pathogen Detection: A Pilot Study

Electrostatic Sampling of Patient Breath for Pathogen Detection: A Pilot Study

Indoor Air Quality in Healthcare and Care Facilities: Chemical Pollutants and Microbiological Contaminants

A microfluidics-based on-chip impinger for airborne particle collection

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