Impact of dietary leaf extracts of Black pepper Piper nigrum L. on the growth, hematological and immunological parameters of Labeo rohita (Hamilton, 1822) cultured in glass aquaria

Ullah, Azmat; Azmat, Hamda; Masood, Zubia; Arooj, Fariha; Hussain, Syed Makhdoom; Malik, Andi Adam

doi:10.1590/1519-6984.246825

Cited by 4 publications

(5 citation statements)

References 24 publications

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“…In glass aquariums, due to the flat and smooth surface, cleaning is easier and more effective (Ullah et al, 2023), removing the eggs to avoid consumption. In aquariums with ceramic floors, due to porosity (Souza et al, 2008) there is greater adhesion of organic matter.…”

Section: Discussionmentioning

confidence: 99%

The growth performance and parasite load of angelfish juveniles Pterophyllum scalare kept at different stocking densities in two rearing systems

Santos,

Zanella,

Ferreira

et al. 2024

Braz. J. Biol.

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This study evaluated the growth performance and parasite load of angelfish juveniles Pterophyllum scalare kept at different stocking densities using two rearing systems. The experiment was conducted in a factorial design (4x2) with four stocking densities (0.1, 0.4, 0.7, and 1.0 g/L), two type of aquarium tanks (glass and ceramic aquariums), and four replicates. The experiment lasted 60 days using 148 juvenile fish (3.05 ± 0.09 g) randomly placed in 32 aquariums (50 L) equipped with filters and aeration. All fish received two meals a day ad libitum (8:00 and 16:00). Water quality parameters such as temperature, dissolved oxygen, pH, and total ammonia were measured. At the end of the experiment, all fish were measured and weighed to determine growth performance and then subjected to parasitological analysis. The data were analyzed with a two-way ANOVA with post-hoc Tukey test (p<0.05). No effects on growth performance at different stocking densities were observed. However, there was an increase in Capillaria pterophylli infestation in the high stocking density within ceramic aquariums. Thus, this study recommends the use of 1.0 g/L for the intensive aquaculture system of freshwater angelfish, and applying cleaning management to avoid parasite infestation, particularly in ceramic aquariums.

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Section: Discussionmentioning

confidence: 99%

The growth performance and parasite load of angelfish juveniles Pterophyllum scalare kept at different stocking densities in two rearing systems

Santos,

Zanella,

Ferreira

et al. 2024

Braz. J. Biol.

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“…( 61 ) showed that the dietary supplementation of red bell pepper ( Capsicum annum ) at an inclusion level of 44 or 55 mg/kg for 20–60 days significantly increased the BW and total length, but not the SGR in rainbow trout ( Oncorhynchus mykiss ). When supplementing fish diets with black pepper or its extracts, growth was not enhanced in African catfish ( Clarias gariepinus ), rainbow trout, and common carp ( Cyprinus carpio ) ( 62 – 64 ), but weight gain was significantly increased in rohu fish ( Labeo rohita ) when adding the extract to the diet at an inclusion level of 1% or 2% ( 65 ). Among the four spices tested in the current study, ginger has been the most studied regarding its growth enhancing effect in teleosts ( 66 – 70 , among others).…”

Section: Discussionmentioning

confidence: 99%

“…( 20 ) when supplementing gilthead seabream diets with 0.1% and 0.15% of this additive during winter. While none of the aforementioned studies testing the effect of capsicum in fish diets described an amelioration of FCR ( 56 , 58 – 60 ), some of the works with black pepper or ginger and many of those with cinnamaldehyde supplementation did ( 63 , 65 , 66 , 68 , 70 , 72 , 74 , 75 ). Therefore, the decrease in FCR in fish fed the SPICY 0.1% diet might be partly attributed to the active principles of ginger and black pepper and to cinnamaldehyde or to the synergetic effect of the four spices present in the tested product.…”

Section: Discussionmentioning

confidence: 99%

“…This has been seen previously when each of the spices was tested individually in mammals ( 107 , 118 ) and is also in line with the anti-inflammatory effect that ginger and cinnamaldehyde apparently have in the intestine of rohu fish and bacteria-infected zebrafish ( Danio rerio ), respectively ( 68 , 119 ). Although black pepper and capsicum have also been reported to act as immunostimulants in fish, such as rainbow trout and rohu fish, respectively ( 65 , 120 , 121 ), their effects on inflammation have not yet been elucidated.…”

Section: Discussionmentioning

confidence: 99%

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The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective

Ruiz,

Sanahuja,

Andree

et al. 2023

Front. Immunol.

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This work studied the potential of a combination of pungent spices (capsicum, black pepper, ginger, and cinnamaldehyde) to be used as a supplement in diets of gilthead seabream (Sparus aurata; 44.1 ± 4.2 g). During 90 days, fish were fed three experimental diets with low inclusion of fish oil and containing poultry fat as the main source of lipids, supplemented with graded levels of the tested supplement: 0 (control), 0.1 (SPICY0.1%), and 0.15% (SPICY0.15%). As a result, the pungent spices enhanced the growth performance, the activity of the bile-salt-activated lipase in the intestine, and decreased fat deposit levels within enterocytes. The SPICY0.1% diet reduced the feed conversion ratio and the perivisceral fat index and lipid deposits in the liver. Moreover, the ratio of docosahexaenoic acid/eicosapentaenoic acid in fillet increased in fish fed the SPICY0.1% diet, while the hepatic levels of docosahexaenoic acid and total n-3 polyunsaturated fatty acids increased in fish fed the SPICY0.15% diet. Furthermore, there was an effect on the expression of some biomarkers related to lipid metabolism in 2-h postprandial fish (fasn, elovl6, scd1b, cyp7a1, lpl, and pparβ), and in 48 h fasted-fish fed with the SPICY0.1% diet, a regulation of the intestinal immune response was indicated. However, no significant differences were found in lipid apparent digestibility and proximate macronutrient composition. The spices did not affect biomarkers of hepatic or oxidative stress. No differences in microbial diversity were found, except for an increase in Simpson’s Index in the posterior intestine of fish fed the SPICY0.1% diet, reflected in the increased relative abundance of the phylum Chloroflexi and lower relative abundances of the genera Campylobacter, Corynebacterium, and Peptoniphilus. In conclusion, the supplementation of gilthead seabream diets with pungent spices at an inclusion of 0.1% was beneficial to enhance growth performance and feed utilization; reduce fat accumulation in the visceral cavity, liver, and intestine; and improve the fish health status and condition. Results suggest that the tested supplement can be used as part of a nutritional strategy to promote a more judicious use of fish oil in fish diets due to its decreasing availability and rising costs.

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“…Furthermore, in this study, supplementation of black pepper showed a slight reduction of plasma hemoglobin and hematocrit compared to Con diet. Ullah et al (2021) showed contradictory results an increase in red blood cells, total leukocyte count and lymphocytes in Labeo rohita with black pepper leaf extract supplementation. Hassan et al (2023) showed that red pepper oil supplementation significantly increased the red blood cells count and hemoglobin in broilers.…”

Section: Fisheries and Aquatic Sciencesmentioning

confidence: 97%

Dietary supplementation of piperine improves innate immunity, growth performance, feed utilization and intestinal morphology of red seabream (Pagrus major)

Hasanthi,

Malintha,

Yun

et al. 2023

Fish Aquat Sci

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Piperine, the main bioactive component of black pepper (Piper nigrum Linn.), has anti-inflammatory, antifungal, and antibacterial properties. This study evaluated the supplemental effects of piperine or black pepper on innate immunity, growth, feed utilization efficiency, and intestinal morphology in red seabream (Pagrus major). Six experimental diets were formulated, supplementing piperine at 0.0, 0.25, 0.5, 1.0, and 2.0 g/kg levels (Con, P25, P50, P100, and P200) or 1.0 g/kg black pepper (BP100). Juvenile fish (7.6 ± 0.1 g) were randomly stocked into 18 circular tanks (220 L), including 30 fish per tank. Each diet was randomly assigned to triplicate groups, and the feeding trial was conducted for 8 weeks. The results showed that final body weight, specific growth rate, weight gain, and feed utilization efficiency were significantly improved (p < 0.05) when piperine was supplemented into diets at 0.25-2.0 g/kg levels compared to the Con group. Compared to the Con diet, condition factor was significantly increased (p < 0.05) in fish fed with dietary piperine at 0.25-2.0 g/kg or BP100 diet. Serum myeloperoxidase activity was increased (p < 0.05) in P25 and P100 groups and antiprotease activity was increased (p < 0.05) in P100 group compared to the Con group. Significantly higher (p < 0.05) lysozyme activity was observed in P50, P100, P200 and BP100 groups, while total immunoglobulin level was increased in P50, P100 and BP100 groups than Con group. Superoxide dismutase activity was increased (p < 0.05) by dietary piperine at 0.25-2.0 g/kg levels and BP100 diet compared to Con diet. Plasma cholesterol was significantly lower (p < 0.05) in fish fed with piperine (0.5-2.0 g/kg) or BP100 compared to the Con diet. Compared to the Con diet significantly longer (p < 0.05) intestinal villi were observed in fish fed with piperine at 0.25-1.0 g/kg levels, and higher goblet cell count was observed in P25 and BP100 groups. Dietary inclusion of piperine would be a potent immunostimulant in fish diet and the optimum supplementation level would be 0.25-1.0 g/kg.

show abstract

Impact of dietary leaf extracts of Black pepper Piper nigrum L. on the growth, hematological and immunological parameters of Labeo rohita (Hamilton, 1822) cultured in glass aquaria

Cited by 4 publications

References 24 publications

The growth performance and parasite load of angelfish juveniles Pterophyllum scalare kept at different stocking densities in two rearing systems

The growth performance and parasite load of angelfish juveniles Pterophyllum scalare kept at different stocking densities in two rearing systems

The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective

Dietary supplementation of piperine improves innate immunity, growth performance, feed utilization and intestinal morphology of red seabream (Pagrus major)

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