Impact of Different Access Cavity Designs and Ni–Ti Files on the Elimination of Enterococcus faecalis from the Root Canal System: An In Vitro Study

Abstract

The present research aimed to reveal the in vitro effects of different endodontic file systems utilizing different endodontic access cavity designs on the elimination of Enterococcus faecalis from root canal systems. One hundred and fifty mandibular molar teeth were separated into three groups with respect to the access cavity design: traditional endodontic cavity (TEC), conservative endodontic cavity (CEC), and truss access cavity (TAC) (n = 50). After access cavity preparation, inolulation of teeth with E. faecalis was undertaken for 21 days. Following the first sampling (S1), further division of the groups into five subgroups (n = 10) was performed: ProTaper Gold, 2Shape, TruNatomy, Reciproc Blue, and WaveOne Gold. After instrumentation, bacterial sampling (S2) was performed. Bacterial reduction was evaluated in colony-forming units/mL, and statistical analysis was carried out. The E. faecalis count decreased significantly in all groups (p < 0.05). The range of mean values for a decrease in E. faecalis in log numbers was lowest in the TAC and Reciproc Blue groups, while the highest values of bacterial reduction were detected in the TEC and ProTaper Gold groups. The highest level of antibacterial action was obtained with the use of ProTaper Gold compared to the other file systems tested.

The effect of different access cavity designs on bacterial biofilm reduction using Er,Cr:YSGG laser in comparison to conventional disinfection protocols. An in vitro study

“…This was in accordance with Andac et al 20 with Shan et al 19 study in which minimal and traditional access cavity designs showed significant difference only while using conventional irrigation alone but both designs showed comparable results and bacterial reduction when NaOCl irrigant was activated using PUI and LAI using Er:YAG laser.…”

“…The use of CFU allowed for multiple and sequential testing of the same sample at different interventional points and, hence, was more suitable for the applied study design. 11,19,20 Regarding access cavity design and mechanical instrumentation with saline, traditional access samples exhibit higher bacterial reduction than contracted access samples. This is attributed to coronal restriction in contracted access, interfering with efficient mechanical instrumentation.…”

The Influence of Minimally Invasive Access Cavities on the Cleaning Ability of Primary Infected Root Canals: An in-Vitro Study

“…In order to understand the in vitro effects of various endodontic file systems utilizing various endodontic access cavity designs on the eradication of Enterococcus faecalis from the root canal systems, another study was conducted recently. In order to understand the in vitro effects of various endodontic file systems utilizing various endodontic access cavity designs on the removal of Enterococcus faecalis from the root canal systems, another study was conducted recently 54 .…”

Biological Potential of the Main Component, Thymoquinone, of Nigella sativa in Pulp Therapy-In Vitro Study

“…Endodontic infections are polymicrobial infections that contain mainly anaerobic bacteria and facultative species [1]. Root canal treatment helps to eradicate microbes and prevent recontamination after chemo-mechanical preparation [2]. During root canal treatment, different irrigants are required to be used.…”