Impact of different combined preservation modalities on warm ischemic kidneys: effect on oxidative stress, hydrostatic perfusion characteristics and tissue damage

Fabre, Elodie; Conti, Marc; Paradis, Valérie; Droupy, S.; Bédossa, Pierre; Legrand, Alain; Galand, Benoît; Eschwège, P.

doi:10.1007/s00240-002-0237-6

Cited by 8 publications

(3 citation statements)

References 11 publications

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“…Our findings on microtubule cold-stability have compelling clinical applications, such as prolonging the survival of organs awaiting transplantation (Maathuis et al, 2007; Niemann et al, 2015). Prolonged cold storage has been shown to induce mild oxidative stress to organs (Fabre et al, 2002). Therefore, the standard cold storage medium, UW solution, is enriched in antioxidant components.…”

Section: Discussionmentioning

confidence: 99%

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications

Ball

Luan

et al. 2018

Cell

115

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Hibernating mammals survive hypothermia (<10°C) without injury, a remarkable feat of cellular preservation that bears significance for potential medical applications. However, mechanisms imparting cold resistance, such as cytoskeleton stability, remain elusive. Using the first iPSC line from a hibernating mammal (13-lined ground squirrel), we uncovered cellular pathways critical for cold tolerance. Comparison between human and ground squirrel iPSC-derived neurons revealed differential mitochondrial and protein quality control responses to cold. In human iPSC-neurons, cold triggered mitochondrial stress, resulting in reactive oxygen species overproduction and lysosomal membrane permeabilization, contributing to microtubule destruction. Manipulations of these pathways endowed microtubule cold stability upon human iPSC-neurons and rat (a non-hibernator) retina, preserving its light responsiveness after prolonged cold exposure. Furthermore, these treatments significantly improved microtubule integrity in cold-stored kidneys, demonstrating the potential for prolonging shelf-life of organ transplants. Thus, ground squirrel iPSCs offer a unique platform for bringing cold-adaptive strategies from hibernators to humans in clinical applications. VIDEO ABSTRACT.

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Section: Discussionmentioning

confidence: 99%

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications

Ball

Luan

et al. 2018

Cell

115

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“…Also, intrarenal resistance in the VSOP group was comparable to that in the controls, in contrast to intrarenal resistance in the OMP group, which increased during reperfusion. Previous studies also showed induction of oxidative stress by OMP that overpowered the antioxidant defenses of the kidney, resulting in increased vascular resistance and tissue damage [46,47]. …”

Section: Discussionmentioning

confidence: 99%

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Kalenski

Mancina²,

Paschenda³

et al. 2016

Eur Surg Res

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Background/Aim: The global shortage of donor organs for transplantation has necessitated the expansion of the organ pool through increased use of organs from less ideal donors. Venous systemic oxygen persufflation (VSOP) and oxygenated machine perfusion (OMP) have previously demonstrated beneficial results compared to cold storage (CS) in the preservation of warm-ischemia-damaged kidney grafts. The aim of this study was to compare the efficacy of VSOP and OMP for the preservation of warm-ischemia-damaged porcine kidneys using the recently introduced Ecosol preservation solution compared to CS using Ecosol or histidine-tryptophan-ketoglutarate solution (HTK). Materials and Methods: Kidneys from German Landrace pigs (n = 5/group) were retrieved and washed out with either Ecosol or HTK after 45 min of clamping of the renal pedicle. As controls, kidneys without warm ischemia, cold stored for 24 h in HTK, were employed. Following 24 h of preservation by VSOP, OMP, CS-Ecosol, or CS-HTK, renal function and damage were assessed during 1 h using the isolated perfused porcine kidney model. Results: During reperfusion, urine production was significantly higher in the VSOP and OMP groups than in the CS-HTK group; however, only VSOP could demonstrate lower urine protein concentrations and fractional excretion of sodium, which did not differ from the non-warm-ischemia-damaged control group. VSOP, CS-Ecosol, and controls showed better maintenance of the acid-base balance than CS-HTK. Reduced lipid peroxidation, as reflected in postreperfusion tissue thiobarbituric acid-reactive substance levels, was observed in the VSOP group compared to the OMP group, and the VSOP and CS-Ecosol groups had concentrations similar to the controls. The ratio of reduced to oxidized glutathione was higher in the VSOP, OMP, and CS-Ecosol groups than in the CS-HTK group and controls, with a higher ratio in the VSOP than in the OMP group. Conclusion: VSOP was associated with mitigation of oxidative stress in comparison to OMP and CS. Preservation of warm-ischemia-damaged porcine kidneys by VSOP was improved compared to OMP and CS, and was comparable to preservation of non-warm-ischemia-damaged cold-stored kidneys.

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“…After intravenous heparinization (300 U), cardiac arrest was induced by opening the chest. The cessation of the heart beat was observed within 3–5 min, and donor animals were kept for 40 min on a 37 degree pad [14,15]. The left kidney then was removed with the left renal artery and a short aortic segment, and the left renal vein with a patch of the vena cava.…”

Section: Methodsmentioning

confidence: 99%

Carbon monoxide inhibits apoptosis during cold storage and protects kidney grafts donated after cardiac death

Ozaki

Yoshida

Ueki

et al. 2011

Transplant International

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Summary Ischemia/reperfusion (I/R) injury remains as a serious deleterious factor in kidney transplantation (KTx). We hypothesized that carbon monoxide (CO), an endogenous potent cytoprotective molecule, inhibits hypothermia‐induced apoptosis of kidney grafts. Using the rat KTx model mimicking the conditions of donation after cardiac death (DCD) as well as nontransplantable human kidney grafts, this study examined effects of CO in preservation solution in improving the quality of marginal kidney grafts. After cardiac cessation, rat kidneys underwent 40 min warm ischemia (WI) and 24 h cold storage (CS) in control UW or UW containing CO (CO‐UW). At the end of CS, kidney grafts in control UW markedly increased mitochondrial porin release into the cytosol and resulted in increased cleaved caspase‐3 and PARP expression. In contrast, grafts in CO‐UW had significantly reduced mitochondrial breakdown and caspase pathway activation. After KTx, recipient survival significantly improved with CO‐UW with less TUNEL+ cells and reduced mRNA upregulation for proinflammatory mediators (IL‐6, TNF‐α, iNOS). Furthermore, when nontransplantable human kidney grafts were stored in CO‐UW for 24 h, graft PARP expression, TUNEL+ cells, and proinflammatory mediators were less than those in control UW. CO in UW inhibited hypothermia‐induced apoptosis and significantly improved kidney graft function and outcomes of KTx.

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Impact of different combined preservation modalities on warm ischemic kidneys: effect on oxidative stress, hydrostatic perfusion characteristics and tissue damage

Cited by 8 publications

References 11 publications

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications

Comparison of Aerobic Preservation by Venous Systemic Oxygen Persufflation or Oxygenated Machine Perfusion of Warm-Ischemia-Damaged Porcine Kidneys

Carbon monoxide inhibits apoptosis during cold storage and protects kidney grafts donated after cardiac death

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