Methods to improve the bioactive component content of cultivated licorice have become the bottleneck of industrial licorice extraction for pharmaceutical use. To evaluate the effects of genotype, environment and their interaction on major bioactive components, we analyzed the five bioactive components: liquiritin (LQ), liquiritigenin (LQG), glycyrrhizin (GL), isoliquiritin (ILQ) and isoliquiritigenin (ILQG) of four diverse licorice varieties grown in four distinct environments in northern China during 2010-11. Analysis of variance showed that environmental and genotypic effects were significant (p<0.01) for all five bioactive components. Additionally, their interaction was significant (p<0.05) for GL in the 2-year study period. LQ and ILQ were mainly affected by genetic factors and have great potential for genetic improvement, whereas LQG and ILQG were mainly affected by environmental factors. GL was similarly affected by environmental and genetic factors. Biplot of the principal component analysis showed that for quality breeding, G2 (WNT-1) and G3 (JX-1) are two relatively preferable genotypes, and E2 (Chifeng) location is suitable for accumulation of the bioactive components of these two genotypes. Stepwise regression analysis showed that sunshine and rainfall are the dominant environmental factors for licorice bioactive component accumulation; increased duration of sunshine is advantageous to GL accumulation whereas declining rainfall is conducive to LQG and ILQG accumulation. These results provide a theoretical basis for initiating licorice breeding programs with increased bioactive components and improved quality.Key words licorice (Glycyrrhiza uralensis); genotype; environment; bioactive component; geographical location; quality breeding Licorice (Glycyrrhiza uralensis FISCH.) belongs to the family Leguminosae, and has been used as a popular herbal medicine for thousands of years in China.1,2) Biological activities of licorice have been attributed to triterpene saponins, various types of flavonoids, such as glycyrrhizin (GL), liquiritin (LQ), liquiritigenin (LQG), isoliquiritin (ILQ) and isoliquiritigenin (ILQG), and other bioactive components, which exhibit anti-inflammatory, anti-viral, anti-allergenic, anti-ulcer and anti-oxidative properties, and are also believed to have chemopreventive activity against cancer and AIDS. [3][4][5] Licorice is one of the most important industrial materials for pharmaceuticals, 6) and the total amount of import and export in China reached 13960 t in 2011.7) More than 90% of total licorice production in 2005 was Glycyrrhiza uralensis.
5)Due to the low content of bioactive components in cultivated populations, licorice is mainly extracted from wild variety despite its limited yield, which has restricted the extent of industrial extraction. With increasing demands for licorice worldwide, wild licorice is unable to meet the global needs.
8)Hence, methods to improve the quality of cultivated licorice have become the bottleneck of industrial licorice extraction. Furthermore, ...