Impact of donor age and weaning status on pancreatic exocrine and endocrine tissue maturation in pigs

Abstract

Background: During the process of islet isolation, pancreatic enzymes are activated and

In vitro characterization of neonatal, juvenile, and adult porcine islet oxygen demand, β‐cell function, and transcriptomes

“…Juvenile Porcine Islets: JPI were isolated by the Lakey laboratory according to previously published protocols. 36,37 JPI were cultured for 5 days prior to assessment. Adult Porcine Islets: API were isolated from a heritage pig breed by the Hering laboratory using the automated method and tissue dissociating enzymes at low temperatures before being purified on continuous iodixanol density gradients as previously described.…”

Production of high‐quality islets from goettingen minipigs: Choice of organ preservation solution, donor pool, and optimal cold ischemia time

“…However, resilient pre-clinical data on NICCs xenotransplantation are still rare. 31,35,36 Alternatively to pig islet sources, research is focusing on generating insulin-producing cells from embryonic stem cells or reprogramming of human induced pluripotent stem cells from somatic cells. Beta cells might also be generated by expansion of pre-existing beta cells, by neogenesis or reprogramming non-islet or somatic cells.…”

Characterization of chelator‐mediated recovery of pancreatic islets from barium‐stabilized alginate microcapsules

“…As previously described by our research, donor pancreases were obtained from two juvenile pre‐weaned Yorkshire pigs (S&S Farms) of both sexes. Pancreases were removed using a rapid surgical procurement (≤10 minutes) and placed in cold (4°C) HBSS (Gibco, cat#24020117) until digestion.…”

“…As previously described, islet equivalence (IE) was obtained by collecting an aliquot of approximately 100 IE, staining them with 1mL dithizone (DTZ, MP Biomedicals, cat#150999) for 5 minutes, and counting them at 25× on a standard stereo microscope (Max Erb) with a 10× eyepiece graticule . IE was multiplied by their dilution factor and divided by gram of digested pancreatic tissue to obtain and IE/g of pancreas.…”