Impact of drinking water supplemented 2-hydroxy-4-methylthiobutyric acid in combination with acidifier on performance, intestinal development, and microflora in broilers

Abstract

In addition to offering methionine, 2-hydroxy-4-methylthiobutyric acid ( HMTBa ) is also an organic acid and shows excellent bacteriostasis. Therefore, 3 experiments were conducted to determine the influence of drinking water supplemented HMTBa in combination with acidifier on performance, intestinal development, and microflora in broilers. The addition of different concentration (0.02–0.20%) of the blend of HMTBa and other acids significantly reduced the pH of water and exerted antimicrobial activity in dose-dependent manner in vitro. The outcomes from animal trial consisting of the drinking water with blended acidifier at 0.00, 0.05, 0.10, 0.15, and 0.20% indicated that the water with 0.15 or 0.20% acidifier resulted in linear and quadratic higher body weight at 42 d, gain and water consumption during 1 to 42 d ( P < 0.05). In experiment 3, responding to graded blended acidifier in drinking water, birds receiving 0.10, 0.15, and 0.20% acidifier decreased the internal pH of gastrointestinal tract and muscle, and exhibited increased duodenal weight, length, villus high, and the ratio of villus high to crypt depth. Drinking water with 0.2% blended acidifier increased the abundance of probiotics ( Bacteroidaceae, Ruminococcaceae , and Lachnospiraceae ) and decreased the account of pathogenic bacteria such as Desulfovibrionaceae . Alternations in gut microflora were closely related to the metabolism of carbohydrate, amino acid, and vitamins. These findings, therefore, suggest that drinking water with 0.10 to 0.13% the combination HMTBa with acidifier might benefit to intestinal development and gut microbiota, and the subsequent produce a positive effect on the performance of broilers.

Effects of coated sodium butyrate on performance, egg quality, nutrient digestibility, and intestinal health of laying hens

“…The effect of CSB on nutrient digestibility may be attributed to CSB improving the intestinal environment and increasing the villus height and the absorption area. Meanwhile, assisting in gut development may be a key contributing factor to an acidifier's favorable impact on feed and growth efficiency ( Guo et al, 2021 ), which could explain why adding CSB improves the performance of laying hens in the present study.…”

Bio-Fermented Malic Acid Facilitates the Production of High-Quality Chicken via Enhancing Muscle Antioxidant Capacity of Broilers

“…Dietary supplementation of a mixture of a free butyrate acidifier and gluconic acid improved gut health, including its morphological structure and microbiome activities, in chickens [ 47 ]. As an organic acid, addition of 2-hydroxy-4-methylthiobutyric acid in drinking water shows excellent bacteriostasis to benefit intestinal development and gut microbiota, and the subsequent performance of broilers [ 48 ]. Moreover, the tibia mass of broilers was improved by acidification of drinking water through the alterations of intestinal barrier and microbiota [ 16 ].…”

The effect of acidifier supplementation on egg production performance and intestinal histology of Japanese quail (Coturnix japonica)

“…The acidifiers make proteins and nutrients more digestible by reducing microbial competition for nutrients and resulting in better chicken growth and performance (Dibner & Buttin, 2002 ). An essential objective of dietary acidification is inhibiting intestinal bacteria from competing with the host for available nutrients and reducing possible toxic bacterial metabolites (Guo et al., 2022 ). Therefore, the objectives of the present study were to examine the effect of dietary supplementation of a newly developed acidifier on the production performance, intestinal histology and different parts of GIT pH in layer Japanese quail.…”

“…The acidifiers make proteins and nutrients more digestible by reducing microbial competition for nutrients and resulting in better chicken growth and performance (Dibner & Buttin, 2002). An essential objective of dietary acidification is inhibiting intestinal bacteria from competing with the host for available nutrients and reducing possible toxic bacterial metabolites (Guo et al, 2022).…”