Impact of Dynamic Culture in the RCCS! Bioreactor on a Three-Dimensional Model of Bone Matrix Formation

The Continuum of Health Risk Assessments

“…A comparison of the main features of various bioreactors (static or dynamic flow condition) commonly used in the foremost culture techniques is presented in Table 4. Horizontally rotating, transparent clinostats, RCCS TM devices efficiently create a unique, highly controlled microenvironment that, by reproducing some aspects of microgravity (simulated microgravity) (Klaus, 2001;Ayyaswamy & Mukundakrishnan, 2007), guarantee the most favourable conditions for cell and tissue culturing (Schwarz et al, 1992), and provide potentially powerful tools to reproduce specific 3D tissue morphogenesis (Mazzoleni et al, 2009). Complex tissue-like 3D constructs, different cell types from various origins and various intact tissue explants have been demonstrated, by our group and others, to be kept efficiently in culture by these bioreactors, even for long periods of time (Unsworth & Lelkes, 1998;Hammond & Hammond, 2001;Vunjak-Novakovic et al, 2002;Nickerson et al, 2007;Cosmi et al, 2009;Steimberg et al, 2009Mazzoleni et al, 2011). Figure 5 shows selected examples of RCCS TM -based tissue culture methods, developed and optimised by our group, and their advantages.…”

From the macroscale to nanostructures: can tissue engineering recreate bone features?

“…In such a 3D hydrodynamic culture conditions, isolated cells or tissues explants remain alive, and express their tissue-specific phenotype from several days up to weeks of culture [106,[108][109][110][111][112]. The RCCS TM bioreactor was also successfully applied, also by our group, to the study of the skeletal system on isolated cells, in the presence or in the absence of scaffolds, or on bone explants [107,[113][114][115][116][117]. This bioreactor was also shown to speed up cell aggregation, osteoblastogenesis, osteoblast differentiation, and subsequent mineralization of mesenchymal or embryonic stem cells [109,115].…”

New Models for the In Vitro Study of Liver Toxicity: 3D Culture Systems and the Role of Bioreactors

“…Complex tissue-like 3D constructs, different cell types from various origins and various intact tissue explants have been demonstrated, by our group and others, to be kept efficiently in culture by these bioreactors, even for long periods of time (Unsworth & Lelkes, 1998;Hammond & Hammond, 2001;Vunjak-Novakovic et al, 2002;Nickerson et al, 2007;Cosmi et al, 2009;Steimberg et al, 2009Mazzoleni et al, 2011). Figure 5 shows selected examples of RCCS TM -based tissue culture methods, developed and optimised by our group, and their advantages.…”