Impact of early inoculation of probiotics to suckling piglets on postweaning diarrhoea – A challenge study with Enterotoxigenic E. Coli F18

Hansen, L.H.B.; Lauridsen, Charlotte; Nielsen, Brian; Jørgensen, L. N.; N, N. Canibe

doi:10.1016/j.animal.2022.100667

Cited by 10 publications

(7 citation statements)

References 28 publications

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“…We found a good correlation between the two methods of measuring ETEC F4 in feces (microbiological enumeration and qPCR), yet qPCR showed a higher number of detectable FaeG gene encoding for the F4 fimbriae than the detectable bacterial from the microbiological enumeration. This finding is in line with the study conducted by Hansen et al (33), with ETEC F18 being measured in that study. The qPCR method detects all cells in a sample, including the dead cells or the DNA of bacteria in the environment (69), whereas when plate counting, only viable cells that are capable of forming colonies on nutrient media are counted.…”

Section: Diarrhea Etec F4 and Enterotoxin Sheddingsupporting

confidence: 94%

“…The est-II gene encoding for STb toxin was detectable at relatively high levels in all treatment groups before ETEC challenge in the present study. This is consistent with the findings in previous research (33,(70)(71)(72). The presence of the est-II gene is most probably associated with non-hemolytic E. coli, since quantification of the FaeG gene and bacterial plate counting followed by serotyping confirmed that neither ETEC O149:F4 nor other hemolytic E. coli were detected (except three pigs in the Ch-Dry in the first block, which showed low levels of FaeG gene) before ETEC challenge.…”

Section: Diarrhea Etec F4 and Enterotoxin Sheddingsupporting

confidence: 92%

“…Haptoglobin analyses were performed using an ADVIA 1800 Clinical Chemistry System autoanalyzer (Siemens Healthineers AG, Germany), while CRP concentrations were determined by solid-phase sandwich immunoassay, and measured using a POLARstar Galaxy microplate reader (BMG Labtech, Germany). The plasma concentration of cytokines (IFN-γ, IL-1β, IL-6, IL-10, IL-12, and TNF-α) were analyzed using methods previously described (33). Plasma diamine oxidase activity (DAO) was determined by a kineticfluorometric method where 1.5 diamino pentane (cadaverine, Sigma C8561) was the substrate and 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) was the profluophore oxidized by the developed hydrogen peroxide.…”

Section: Hematology and Immune Parametersmentioning

confidence: 99%

“…We observed a great individual variability, which was previously reported by Pomorska-Mól (73) and Yu et al (74), indicating the difference in reactions to stress among individuals. Our experience with acute phase proteins analyses indicate variation from batch to batch of pigs used in ETEC challenge studies conducted in the same facility (33). Moreover, the acute phase proteins reference ranges/ threshold values for ETEC infection are still unclear due to different methods applied in different institutions.…”

Section: Blood Parametersmentioning

confidence: 99%

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Liquid fermented cereals with added Pediococcus acidilactici did not reduce post-weaning diarrhea in pigs – an Escherichia coli challenge study

Noel

Lauridsen

et al. 2023

Front. Vet. Sci.

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The effect of feeding fermented liquid feed (FLF) with added Pediococcus acidilactici to weaning piglets challenged with enterotoxigenic Escherichia coli (ETEC) F4 on aspects of diarrhea, performance, immune responses, and intestinal epithelial barrier function was investigated. A total of 46 weaners (weaning at 27–30 days of age) were assigned to four treatments: (1) Non-challenged and dry feed (Non-Dry); (2) Challenged and dry feed (Ch-Dry); (3) Non-challenged and FLF (Non-Ferm); (4) Challenged and FLF (Ch-Ferm). All groups received the same feed, either dry (Non-Dry and Ch-Dry), or in liquid form (Non-Ferm and Ch-Ferm) in which the cereals with added P. acidilactici (106 CFU/g cereals) had been fermented for 24 h at 30°C. On day 1 and 2 post weaning, Ch-Dry and Ch-Ferm were orally inoculated with 5 mL × 109 CFU ETEC F4/mL, whereas the Non-Dry and Non-Ferm received the same amount of saline. Fecal samples and blood samples were collected through the study period. The microbial composition, concentration of microbial metabolites and nutrient composition indicated that the quality of the FLF was high. In the first week, ADFI of both non-challenged groups was significantly higher (p < 0.05) than that of the Ch-Ferm group. The two challenged groups had higher fecal levels of FaeG gene (ETEC F4 fimbriae) from day 2 to 6 post weaning (p < 0.01), and higher risk of having ETEC F4 present in feces from day 3 to 5 post weaning (p < 0.05) compared to non-challenged groups, indicating the validity of the ETEC challenge model. Generally, ADG of the two groups fed FLF were numerically higher than those fed dry feed. Neither challenge nor FLF affected diarrhea. No significant differences were measured between Ch-Ferm and Ch-Dry regarding the level of plasma haptoglobin and C-reactive protein, hematological parameters or parameters related to epithelial barrier. The data indicated a low level of infection caused by the ETEC challenge, while recovery from weaning stress could be observed. The study showed that a strategy like this can be a way of providing a high level of probiotics to pigs by allowing their proliferation during fermentation.

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Section: Diarrhea Etec F4 and Enterotoxin Sheddingsupporting

confidence: 94%

Section: Diarrhea Etec F4 and Enterotoxin Sheddingsupporting

confidence: 92%

Section: Hematology and Immune Parametersmentioning

confidence: 99%

Section: Blood Parametersmentioning

confidence: 99%

See 2 more Smart Citations

Liquid fermented cereals with added Pediococcus acidilactici did not reduce post-weaning diarrhea in pigs – an Escherichia coli challenge study

Noel

Lauridsen

et al. 2023

Front. Vet. Sci.

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“…To our knowledge, our study is also the only one to date to show lifetime benefits in the offspring of probiotic-supplemented sows, namely improved growth during the finisher period and increased carcass weight at slaughter [ 109 ]. Studies also demonstrate that the administration of probiotics directly to suckling piglets can accelerate the response to enterotoxigenic E. coli challenge post-weaning [ 110 ] and can increase pre-weaning and early post-weaning pig growth, possibly via gut microbiota/immune modulation [ 111 , 112 ].…”

Section: Nutritional Strategies In Suckling Pigs To Improve Growth An...mentioning

confidence: 99%

Selected Nutrition and Management Strategies in Suckling Pigs to Improve Post-Weaning Outcomes

Arnaud

Gardiner

Lawlor

2023

Animals

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Weaning is a critical period in a pig’s life. Piglets are confronted with abrupt changes to their physical and social environment, as well as management and nutritional changes. Weaning has always been associated with a growth check and is frequently accompanied by post-weaning diarrhoea in piglets. However, rapid increases in litter size in the last decade have increased within-litter piglet weight variation, with piglets now generally lighter at weaning, making the challenges associated with weaning even greater. Many interventions can be employed during the suckling period to ease the weaning transition for piglets. Pre-weaning strategies such as supervised farrowing (assistance with suckling and oxytocin provision), the provision of pain relief to sows around farrowing, split-suckling, early oral supplementation with glucose, bovine colostrum, faecal microbiota transplantation, feed additives and solid and liquid creep feeding (milk and liquid feed) have all been investigated. The objective of these strategies is to stimulate earlier maturation of the digestive tract, improve immunity, reduce latency to the first feed post-weaning and increase early post-weaning feed intake and growth. This review focuses in particular on: (1) pain relief provision to sows around farrowing, (2)split-suckling of piglets, (3) pre-weaning provision of supplementary milk and/or liquid feed, (4) other strategies to stimulate earlier enzyme production (e.g., enzyme supplementation), (5) other nutritional strategies to promote improved gut structure and function (e.g., L-glutamine supplementation), and (6) other strategies to modulate gut microbiota (e.g., probiotics and prebiotics). Correctly implementing these strategies can, not only increase post-weaning growth and reduce mortality, but also maximise lifetime growth in pigs.

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Gastrointestinal microbiome engineering in pig

Veerapagu,

Jeya,

Sankara Narayanan

2025

Human and Animal Microbiome Engineering

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Impact of early inoculation of probiotics to suckling piglets on postweaning diarrhoea – A challenge study with Enterotoxigenic E. Coli F18

Cited by 10 publications

References 28 publications

Liquid fermented cereals with added Pediococcus acidilactici did not reduce post-weaning diarrhea in pigs – an Escherichia coli challenge study

Liquid fermented cereals with added Pediococcus acidilactici did not reduce post-weaning diarrhea in pigs – an Escherichia coli challenge study

Selected Nutrition and Management Strategies in Suckling Pigs to Improve Post-Weaning Outcomes

Gastrointestinal microbiome engineering in pig

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