Impact of engineered nanoparticles on the fate of antibiotic resistance genes in wastewater and receiving environments: A comprehensive review

Cui, Hanlin; Smith, Adam L.

doi:10.1016/j.envres.2021.112373

Cited by 31 publications

(11 citation statements)

References 114 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, the production of reactive oxygen species (ROS) may play a role in upregulating competence genes and facilitating gene transfer. − While there has been no evidence that SiO 2 produces ROS in associated bacteria, humic acids are known photocatalytic producers of singlet oxygen and may have played a role in facilitating gene uptake . This effect may have been more pronounced for HASNPs, which have a greater surface area to volume ratio and can bind comparatively more organics than HASPs.…”

Section: Resultsmentioning

confidence: 99%

Investigating and Modeling the Regulation of Extracellular Antibiotic Resistance Gene Bioavailability by Naturally Occurring Nanoparticles

Chowdhury

Hicks

Wiesner

2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

Extracellular antibiotic resistance genes (eARGs) are widespread in the environment and can genetically transform bacteria. This work examined the role of environmentally relevant nanoparticles (NPs) in regulating eARG bioavailability. eARGs extracted from antibiotic-resistant B. subtilis were incubated with nonresistant recipient B. subtilis cells. In the mixture, particle type (either humic acid coated nanoparticles (HASNPs) or their micron-sized counterpart (HASPs)), DNase I concentration, and eARG type were systematically varied. Transformants were counted on selective media. Particles decreased bacterial growth and eARG bioavailability in systems without nuclease. When DNase I was present (≥5 μg/mL), particles increased transformation via chromosomal (but not plasmid-borne) eARGs. HASNPs increased transformation more than HASPs, indicating that the smaller nanoparticle with greater surface area per volume is more effective in increasing eARG bioavailability. These results were also modeled via particle aggregation theory, which represented eARG–bacteria interactions as transport leading to collision, followed by attachment. Using attachment efficiency as a fitting factor, the model predicted transformant concentrations within 35% of experimental data. These results confirm the ability of NPs to increase eARG bioavailability and suggest that particle aggregation theory may be a simplified and suitable framework to broadly predict eARG uptake.

show abstract

Section: Resultsmentioning

confidence: 99%

Investigating and Modeling the Regulation of Extracellular Antibiotic Resistance Gene Bioavailability by Naturally Occurring Nanoparticles

Chowdhury

Hicks

Wiesner

2022

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Studies have shown that oxidative damage is the main mechanism by which most nanoparticles kill microorganisms 22–24 . Therefore, we speculated that SeNPs@CS killed PWNs and destroyed the tissue structure by producing ROS.…”

Section: Methodsmentioning

confidence: 96%

“…Studies have shown that oxidative damage is the main mechanism by which most nanoparticles kill microorganisms. [22][23][24] Therefore, we speculated that SeNPs@CS killed PWNs and destroyed the tissue structure by producing ROS. The production of ROS was detected in this study by using H2DCFDA, which can react with ROS produced in cells to produce 2 0 ,7 0 -dichlorofluorescein, which shows green fluorescence.…”

Section: Analysis Of Oxygen Free Radicals (Ros)mentioning

confidence: 99%

Selenium nanoparticles are effective in penetrating pine and causing high oxidative damage to Bursaphelenchus xylophilus in pine wilt disease control

Shang

Zhang

Zhao

et al. 2022

Pest Management Science

View full text Add to dashboard Cite

BACKGROUND: Research on selenium nanoparticles (SeNPs) in chemical defense and chemotherapy of plants has developed rapidly owing to their high microbial toxicity, environmental safety, and degradability. Pine wilt disease (PWD) threatens pine forests worldwide; however, it is difficult to kill the nematodes (Bursaphelenchus xylophilus) inside the tree that cause PWD using traditional pesticide formulations. SeNPs could be the key to controlling PWD.RESULTS: In this study, approximately 50 nm SeNPs were prepared using a simple and green method, and chitosan was used to increase their biocompatibility and stability. The preparation and characterization results showed that the prepared SeNPs coated with chitosan (SeNPs@CS) were spherical and evenly dispersed. The bioassay results showed that SeNPs@CS had an LC 50 of 15.627 mg L −1 against B. xylophilus. In addition, the killing mechanism of SeNPs@CS against B. xylophilus was studied. Confocal microscopy and transmission electron microscopy demonstrated that B. xylophilus were killed by reactive oxygen species, and the penetration of nano-form materials to B. xylophilus was higher than that of non-nano-form materials. To verify the effective penetration of SeNPs in pine tissues, Cy5-labeled SeNPs@CS was observed inside pine needles and branches using frozen sections and confocal microscopy. In addition, the cytotoxicity of SeO 2 and SeNPs@CS was tested, and the results showed that the cytotoxicity of SeNPs@CS to MC3T3-E1 cells was reduced.CONCLUSION: These results show that SeNPs are expected to be used as a new strategy for the control of PWD with oxidative damage and high penetration to B. xylophilus and effective target penetration and biosafety.

show abstract

“…At low concentrations they are known to promote ARG and HGT in microbes of waste waters and at different concentration of nanoparticles can also cause reduction in ARG and HGT. Nowadays, nanoparticles are widely used in the industry, and they are leached into the wastewater and could reach water bodies raising the concern to the contamination issues [51]. In addition to nanoparticles heavy metal pollutants in wastewaters such as mercury, cadmium, arsenic play a role in increasing the ARG by HGT [50][51][52][53].…”

Section: Processes/treatments That Can Reduce Argsmentioning

confidence: 99%

“…Nowadays, nanoparticles are widely used in the industry, and they are leached into the wastewater and could reach water bodies raising the concern to the contamination issues [51]. In addition to nanoparticles heavy metal pollutants in wastewaters such as mercury, cadmium, arsenic play a role in increasing the ARG by HGT [50][51][52][53]. The metals make the bacterial membrane leaky, increase the expression of genes that can increase conjugation and DNA replication process that helps in the formation of conjugation tube between the donor and recipient bacteria.…”

Section: Processes/treatments That Can Reduce Argsmentioning

confidence: 99%

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Prasad

Elchuri

2022

Chemistry-Didactics-Ecology-Metrology

View full text Add to dashboard Cite

Pharmaceuticals and personal care products (PPCPs) discharged into environment has several adverse impacts. PPCPs are widely utilised for veterinary as well as cosmetic and personal health reasons. These are members of the expanding class of substances known as Contaminants of Emerging Concern (CECs). Antibiotic resistance in the environment and garbage generated by PPCP endanger life. The World Health Organisation (WHO) now recognises antibiotic resistance as a significant global health problem due to the expected increase in mortality caused by it. In the past ten years, mounting data has led experts to believe that the environment has a significant impact on the development of resistance. For human diseases, the external environment serves as a source of resistance genes. It also serves as a major pathway for the spread of resistant bacteria among various habitats and human populations. Large-scale DNA sequencing methods are employed in this thesis to better comprehend the dangers posed by environmental antibiotic resistance. The quantification of the number is an important step in this process. Metagenomic measurement of the number of antibiotic resistance genes in various contexts is a crucial step in this process. However, it’s also crucial to put this data into a broader context by integrating things like taxonomic information, antibiotic concentrations, and the genomic locations of found resistance genes.

show abstract

Impact of engineered nanoparticles on the fate of antibiotic resistance genes in wastewater and receiving environments: A comprehensive review

Cited by 31 publications

References 114 publications

Investigating and Modeling the Regulation of Extracellular Antibiotic Resistance Gene Bioavailability by Naturally Occurring Nanoparticles

Investigating and Modeling the Regulation of Extracellular Antibiotic Resistance Gene Bioavailability by Naturally Occurring Nanoparticles

Selenium nanoparticles are effective in penetrating pine and causing high oxidative damage to Bursaphelenchus xylophilus in pine wilt disease control

Pharmaceuticals and Personal Care Products in the Environment with Emphasis on Horizontal Transfer of Antibiotic Resistance Genes

Contact Info

Product

Resources

About