Impact of Environmental Factors on the Production of Silver Nanoparticles by Saccharomyces Ellipsoideus BSU-XR1

Abstract

In our presented work we have studied the effect of various environmental factors on the synthesis of silver nanoparticles by Saccharomyces ellipsoideus BSU-XR1. Silver nanoparticles were formed by growing the yeast in broth medium at 30oC. Filters were used to seperate biomass from broth. Biomass was washed 3 times with 0.1L of distilled water (DW). 10 grams of wet biomass was poured to beaker containing 90 ml of sterile DW. 1 ml of 10-3 molar solution of Silver nitrate was poured to it, and this mixture with silver nitrate salt was incubated in a dark environment. It was determined that, the optimal condition for the production of silver nanoparticles was being on the 21st day of incubation, in 10 grams of wet biomass. For the cultivation of the studied yeast strain, the synthesis of silver nanoparticles took place in the range of 25-30°C. Tthe initial pH of the medium was 7.0. and the concentration of silver nitrate was used as salt at conntration of 0.5 mM and 1.0 mM. The optimal conditions of incubation were in dark environment in all variotions of experiments.