Impact of environmental stress desiccation, acidity, alkalinity, heat or cold on antibiotic susceptibility of Cronobacter sakazakii

Al‐Nabulsi, Anas A.; Osaili, Tareq M.; Elabedeen, Noor A. Zain; Jaradat, Ziad W.; Shaker, Reyad R.; Kheirallah, Khalid A.; Tarazi, Yaser H.; Holley, Richard A.

doi:10.1016/j.ijfoodmicro.2011.02.013

Cited by 64 publications

(34 citation statements)

References 37 publications

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“…For example, Cronobacter sakazakii, an emerging food-borne pathogen, shows different tolerance toward antibiotics when exposed to desiccation or heat (27). In another example, expression of Anhydrin and LEA-1 peptide stabilizers in the nematode Aphelenchus avenae are markedly up-regulated by dehydration but not by heat (11).…”

Section: Discussionmentioning

confidence: 99%

Impact of trehalose transporter knockdown onAnopheles gambiaestress adaptation and susceptibility toPlasmodium falciparuminfection

Liu

Dong

Huang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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Anopheles gambiae is a major vector mosquito for Plasmodium falciparum, the deadly pathogen causing most human malaria in sub-Saharan Africa. Synthesized in the fat body, trehalose is the predominant sugar in mosquito hemolymph. It not only provides energy but also protects the mosquito against desiccation and heat stresses. Trehalose enters the mosquito hemolymph by the trehalose transporter AgTreT1. In adult female A. gambiae, AgTreT1 is predominantly expressed in the fat body. We found that AgTreT1 expression is induced by environmental stresses such as low humidity or elevated temperature. AgTreT1 RNA silencing reduces the hemolymph trehalose concentration by 40%, and the mosquitoes succumb sooner after exposure to desiccation or heat. After an infectious blood meal, AgTreT1 RNA silencing reduces the number of P. falciparum oocysts in the mosquito midgut by over 70% compared with mock-injected mosquitoes. These data reveal important roles for AgTreT1 in stress adaptation and malaria pathogen development in a major vector mosquito. Thus, AgTreT1 may be a potential target for malaria vector control.

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Section: Discussionmentioning

confidence: 99%

Impact of trehalose transporter knockdown onAnopheles gambiaestress adaptation and susceptibility toPlasmodium falciparuminfection

Liu

Dong

Huang

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

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“…In contrast, Lai (2001) found that all Cronobacter isolates were uniformly resistant to ampicillin, cefazolin and extended-spectrum penicillins, whereas they were uniformly susceptible to trimethoprim/sulfamethoxazole and aminoglycosides. Recently, Al-Nabulsi et al (2011) showed that streptomycin, gentamicin, kanamycin and ciprofloxacin are effective against both stressed and unstressed C. sakazakii cells, and thus these antibiotics might be appropriate choices for patient treatment regimes. Nevertheless, Nazarowec-White & Farber (1999) found that two Cronobacter strains out of eight were resistant to tetracycline and chloramphenicol.…”

Section: Antibiotic Susceptibility and Resistancementioning

confidence: 99%

“…Nonetheless, adequate pasteurization conditions are sufficient to inactivate Cronobacter spp. during processing and reconstitution of PIF (Al-Nabulsi et al, 2011). Recently, an 18 kbp region containing 22 ORFs that are upregulated under heat adaptation conditions has been reported (Gajdosova et al, 2011).…”

Section: Heat Adaptationmentioning

confidence: 99%

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Jaradat

Mousa

Elbetieha

et al. 2014

Journal of Medical Microbiology

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105

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The genus Cronobacter consists of a diverse group of Gram-negative bacilli and comprises seven species: Cronobacter sakazakii, Cronobacter malonaticus, Cronobacter muytjensii, Cronobacter turicensis, Cronobacter dublinensis, Cronobacter universalis and Cronobacter condimenti. Cronobacter are regarded as opportunistic pathogens, and have been implicated in newborn and infant infections, causing meningitis, necrotizing enterocolitis and bacteraemia or sepsis. Cronobacter virulence is believed to be due to multiple factors. Some strains were found to produce diarrhoea or cause significant fluid accumulation in suckling mice. Two iron acquisition systems (eitCBAD and iucABCD/iutA), Cronobacter plasminogen activator gene (cpa), a 17 kb type VI secretion system (T6SS), and a 27 kb filamentous haemagglutinin gene (fhaBC) and associated putative adhesins locus are harboured on a family of RepFIB-related plasmids (pESA3 and pCTU1), suggesting that these are common virulence plasmids; 98 % of 229 tested Cronobacter strains possessed these plasmids. Even though pESA3 and pCTU1 share a common backbone composed of the repA gene and eitCBAD and iucABCD/iutA gene clusters, the presence of cpa, T6SS and FHA loci depended on species, demonstrating a strong correlation with the presence of virulence traits, plasmid type and species. Other factors were observed, in that Cronobacter form biofilms, and show unusual resistance to heat, dry and acid stress growth conditions. The outer-membrane protein A is probably one of the bestcharacterized virulence markers of Cronobacter. Furthermore, it was reported that Cronobacter employ phosphatidylinositide 3-kinase/Akt signalling, which activates protein kinase C-a and impairs the host cell's mitogen-activated protein kinase pathway, in order to invade cells. Cronobacter can also use immature dendritic cells and macrophages to escape the immune response. This review addresses the various virulence and environmental-adaptive characteristics possessed by members of the genus Cronobacter.

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“…Minimum Inhibitory (MIC) and Minimum Bactericidal (MBC) concentrations of amurca against tested organisms were determined as described by Al-Nabulsi et al (2011) in Mueller Hinton (MH) broth (Himedia Ltd., Mambai, India). Ninety six well microtiter plates (Greiner Bio-Dne, CellStar™, Italy) were used to evaluate the effects of different concentrations of methanolic amurca extract on the viability of foodborne pathogens in the present study.…”

Section: Antimicrobial Assaymentioning

confidence: 99%

Antimicrobial activity of amurca (olive oil lees) extract against selected foodborne pathogens

Janakat¹,

Al‐Nabulsi²,

Allehdan³

et al. 2015

Food Sci. Technol (Campinas)

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The antimicrobial activity of a methanolic extract of amurca (olive oil lees) was determined against both Gram-positive (L. monocytogenes and S. aureus) and Gram-negative (E. coli D157:H7 and S. enteritidis) foodborne pathogens at 10 °C or 37 °C using microdilution and disk diffusion methods, and its relative activity was compared to selected antibiotics. Minimum inhibitory (MIC) and minimum bactericidal (MBC) concentrations of amurca extract ranged from 60 to 80 µl/ml at 37 °C after 24 h against all tested strains. At 10 °C, amurca was more inhibitory with MIC and MBC values of 40 and 60 µl/ml, respectively, after 7 d against tested strains. Amurca at 40 µl/ml reduced numbers of tested pathogens by 2.5 to 3.2 log 10 CFU/ml at 10 °C after 7 d, but was not inhibitory at 37 °C after 24 h. Protein prepared from amurca was not antimicrobial. The relative antimicrobial activity (inhibition zone ratio) of 80 µl/ml amurca methanolic extract compared to chloramphenicol, erythromycin, gentamycin and tetracycline ranged from 0.36 to 1.0 against Gram-negative and from 0.45 to 2.0 against Gram-positive bacteria. In addition, amurca extract inhibited E. coli D157:H7 02-0628 and S. aureus 26127 which were resistant to tetracycline and chloramphenicol, respectively.

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Impact of environmental stress desiccation, acidity, alkalinity, heat or cold on antibiotic susceptibility of Cronobacter sakazakii

Cited by 64 publications

References 37 publications

Impact of trehalose transporter knockdown onAnopheles gambiaestress adaptation and susceptibility toPlasmodium falciparuminfection

Impact of trehalose transporter knockdown onAnopheles gambiaestress adaptation and susceptibility toPlasmodium falciparuminfection

Cronobacter spp. – opportunistic food-borne pathogens. A review of their virulence and environmental-adaptive traits

Antimicrobial activity of amurca (olive oil lees) extract against selected foodborne pathogens

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