Impact of epidermal growth factor and/or β-mercaptoethanol supplementations on the in vitro produced buffaloes' embryos

Abstract

The present study investigated the effects of epidermal growth factors (EGF) and/or β-Mercaptoethanol (βME) supplementations to oocyte maturation, fertilization, and culture media on the buffalo in vitro embryo production. The ovaries were collected and transferred within 2 h to the laboratory. The cumulus oocytes complexes were aspirated from 3 to 8 mm diameter follicles. Firstly, EGF; 0, 10, 20, or 50 ng/mL or βME; 0, 25, 50, 100, or 200 μM were supplemented to the in vitro maturation (TCM-199), fertilization (IVF-TALP), or culture (IVC: SOF) media. Our results revealed that supplementing EGF (20 ng/mL) to the TCM-199, IVF-TALP, or SOF media could efficiently improve the growth rates and development of buffalos' embryos, while EGF (50 ng/mL) could stimulate the embryo production only after treatment of the IVF-TALP /or SOF media, but not the IVM medium. However, βME was less efficient than EGF; it stimulated the growth rates of buffalo embryos when supplemented with the maturation and fertilization (IVF-TALP) media in a 50 μM concentration. Secondly, combined EGF (20 ng/mL) and βME (50 μM) were supplemented to the maturation media as effective concentration. The combined treatment of EGF (20 ng/mL) and βME (50 μM) showed no significant enhancing effect on the buffalo embryos compared to each alone. For future perspectives, further study is required to examine the effects of combined EGF and βME on the maturation and fertilization of buffalo oocytes at different categories of age and seasonal localities.

addition of histidine and L-tyrosine to maturation and culture media on IVM, IVF and IVC of buffalo oocytes and embryos

“…The embryos in the control groups were unable to develop into a morula or blastocyst in the SOF and FertiCult, while supplementation of L-tyrosine to SOF or Ferticult at different concentrations enhanced the embryonic development (morula and blastocysts rates) In the same line, early cleavage rate was higher in media containing high content of essential amino acids, while more cleaved ova complete its development when cultured in medium with a lower concentration of essential amino acids [45]. It was found that, amino acids (especially L-gluatmine) and vitamin constituent of Ham's F-10 and TCM-199 have a bene cial effect on embryo development [10,46]. Also, culture for the rst 72 h in nonessential amino acids and glutamine stimulated the cleavage to the morula stage and subsequent blastocyte development [47].…”

“…The nal wash must be done in the in vitro maturation medium (pH 7.2-7.4) before the in vitro maturation droplets are pooled. The oocytes (10)(11)(12)(13)(14)(15)(16)(17)(18)(19)(20) were placed in 50-100µl droplet of maturation medium in a four-well culture plate (Falcon, USA), covered with sterilized light mineral oil (Sigma, USA).…”

“…One of the rst media designed for this purpose was synthetic oviduct uid (SOF), which was established from the composition of bovine oviductal uid [9,10]. SOF medium, which was originally based upon the biochemical and physiological analysis of sheep oviductal uid [11], has been used in the IVM of oocytes in bovine [12,13], goat [14], bitch [15] and in IVC of sheep embryos [16].…”

The Possible Protective Role of Aqueous Extract of Propolis against Hepatotoxicity, Renal and Testicular Toxicity Induced by Cadmium Chloride in Male White Rats

“…The two sections show the renal glomeruli (G) interstitial by Bowman's capsule (BC), the proximal and distal convoluted tubules (PCT), and the distal convoluted tubules (DCT) in normal form, as well as the presence of normal blood vessels between the tubules Nephrotic with the absence of pathological (Figure 6a, b, c, d, e, f ). The intake of cadmium in rats led to a significant decrease in the level of antioxidants such as (superoxide dismutase, catalase, glutathione peroxidase and Glutathione S-transferase) in liver and kidney tissues when compared to the control group, and cadmium also led to a significant increase in the level of lipid peroxidation in the tissues of the liver and kidney when compared to the control group due to its production of many free radicals, which reduces the total antioxidants capacity due to their consumption in dealing with free radicals, leading to a significant increase in the level of oxidative stress and this is considered the cause of hepatic and renal toxicity of cadmium (Shrivastava et al, 2017;Hussein et al, 2022;Ahmed et al, 2023).…”