Since the last few decades, a development of new types of fibers were appeared in order to improve the mechanical properties of fabrics besides its comfort properties, Cotton material was used as a blended material to enhance the properties of the manufactured products. The main purpose of this article is to investigate the influence of different polyester yarn cross-section and gauges on the mechanical properties of blended knitted samples. In this study two groups of knitted samples were manufactured, using gauge 24 and gauge 20, the samples were implemented with single jersey knitted structure by two different polyester microfiber cross-sections and blended cotton ratio. Some of mechanical properties were carried out for all samples such as air permeability, spray test, bursting strength and roughness. The result values recorded and tabulated, as well as statistically analysis using ANOVA Two-way Measurements were executed. The results illustrate that tests properties of blended knitted samples were significantly affected by variables except the spray test. In the same context the results clarify that trilobal cross-section improves UPF property for knitted samples. Finally, due to study the performance of each sample the radar chart was occurred, as the results revealed that Polyester Trilobal samples recorded the best sample radar area with different gauges.