The primary objective of the present study is measuring the consequences of perceived organizational culture on job satisfaction experienced by employees of the hotel industry. The employee owns a set of behavior and attitudes in the organization, termed as organizational culture, influences an employee's job satisfaction. Furthermore, the perception of employees towards the organization's working environment represents a job satisfaction, maybe reflected as positive or negative responses. Thus, these two different concepts have a significant effect on employees. The present study has been conducted on employees working as middle-level managers and managers in five-star hotels of northern India. To assess the relationship between organizational culture and job satisfaction, multiple correlation and stepwise multiple regression analysis has been implemented. Job satisfaction of the employees found positively correlated with all variables of organization culture among employees. The results suggested that openness, authenticity, and experimentation have an insignificant effect on job satisfaction. Further, pro-action, confrontation, and autonomy identified as the most dominant predictors for the job satisfaction of the hotel employees.