Impact of genomics on research in the rat

Lazar, Jozef; Moreno, Carol; Jacob, Howard J.; Kwitek, Anne E.

doi:10.1101/gr.3744005

Cited by 49 publications

(34 citation statements)

References 137 publications

(115 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Naturally, the application of proteomics has also revolutionized the study of animal models mimicking human diseases. A better understanding of animal models of disease through genomics, proteomics and metabolomics is already clearly evident in the field of biomedicine [27][28][29]. Since the screening of patient samples usually requires very large experimental repeats due to considerable interindividual differences in humans, the usage of inbred and therefore genetically more homogeneous animal models is certainly advantageous in the initial search for new biomarkers of pathological processes.…”

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of animal models mimicking skeletal muscle disorders

Doran

Gannon

O’Connell

et al. 2007

Proteomics Clinical Apps

View full text Add to dashboard Cite

Over the last few decades of biomedical research, animal models of neuromuscular diseases have been widely used for determining pathological mechanisms and for testing new therapeutic strategies. With the emergence of high-throughput proteomics technology, the identification of novel protein factors involved in disease processes has been decisively improved. This review outlines the usefulness of the proteomic profiling of animal disease models for the discovery of new reliable biomarkers, for the optimization of diagnostic procedures and the development of new treatment options for skeletal muscle disorders. Since inbred animal strains show genetically much less interindividual differences as compared to human patients, considerably lower experimental repeats are capable of producing meaningful proteomic data. Thus, animal model proteomics can be conveniently employed for both studying basic mechanisms of molecular pathogenesis and the effects of drugs, genetic modifications or cell-based therapies on disease progression. Based on the results from comparative animal proteomics, a more informed decision on the design of clinical proteomics studies could be reached. Since no one animal model represents a perfect pathobiochemical replica of all of the symptoms seen in complex human disorders, the proteomic screening of novel animal models can also be employed for swift and enhanced protein biochemical phenotyping.

show abstract

Section: Introductionmentioning

confidence: 99%

Proteomic profiling of animal models mimicking skeletal muscle disorders

Doran

Gannon

O’Connell

et al. 2007

Proteomics Clinical Apps

View full text Add to dashboard Cite

show abstract

“…23; rat QTLs can be found at http:// rgd.mcw.edu/). Although none of these QTLs directly reflect parameters associated with placentation, several impact growth, vascular function, and metabolism, which could indirectly impact placental development.…”

Section: Discussionmentioning

confidence: 99%

Chromosome-substituted rat strains provide insights into the genetics of placentation

Konno

Rempel

Rumi

et al. 2011

Physiological Genomics

View full text Add to dashboard Cite

show abstract

“…Rats have been used for biomedical and genomic research for long time and a useful model for human disease (26,44). Currently, many inbred mutant and genetically modified rat strains are not available to researchers, and many mutant rat strains have been lost because of inadequate resources to maintain breeding population (1).…”

Section: Introductionmentioning

confidence: 99%

Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain

AGCA¹

2015

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:The objective of this study was to determine the appropriate cooling rates and extender for improving the freezability of epididymal sperm of Lewis rat strain. The epididymal sperm from Lewis rats were suspended in different freezing extenders which were HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), 3% dehydrated skim milk (SM), Salamon's Tris-citrate (TRIS), and TES/Tris (TES). The extenders contained 20% (v/v) egg yolk (EY), 0.75% equex paste (EP) and 0.1 M raffinose or 0.1 M sucrose. The sperm samples were frozen by automated freezer, using various cooling rates (10,40, 70, and 100 °C/min). After thawing, sperm samples were examined for integrity of plasma and acrosomal integrity, and mitochondrial membrane potential (MMP). Type of extenders had significant effect on cryosurvival (p<0.05). The post-thaw highest motility (27.5%) was achieved for the sperm samples frozen in the presence of TES extender (p<0.05). As a result, although cooling rate did not significantly affect cryopreservation, the extenders affected post-thaw sperm parameters and TES considerably improved the freezability of epididymal Lewis rat sperm.Key words: Cooling rate, epididymal sperm, extenders, freezing, Lewis rat strain. Lewis ırkı rat sperması üzerine değişik sulandırıcı ve dondurma hızının etkisiÖzet: Çalışmanın amacı Lewis ırkı rat sperması için, uygun dondurma hızı ve sulandırıcılar tespit ederek spermanın dondurulabilirliğinin artırılmasıdır. Ratlardan alınan epididimal sperma HEPES buffered Tyrode's lactate (TL-HEPES), modified Kreb's Ringer bicarbonate (mKRB), %3 süt tozu (SM), Tris-citrate (TRIS) ve Tris/TES (TES) solüsyonları ile sulandırıldı. Sulandırıcılara 20% (v/v) yumurta sarısı, 0.75% Equex Paste ve 0.1 M rafinoz yada 0.1 M sükroz ilave edildi. Sperma örnekleri kontrollü dondurucu kullanılarak değişik soğutma hızında (10, 40, 70 ve 100 °C/dk) donduruldu. Dondurma sonrası spermalar plazma, akrozom ve mitokondri membran bütünlüğü yönünden değerlendirildi. Sulandırıcı tipinin dondurmada etkili olduğu saptandı (p<0.05). Dondurma-çözüm işlemi sonrası en yüksek motilite (%27.5) TES sulandırıcısından elde edildi (p<0.05). Sonuç olarak, dondurma hızının sperm dondurulmasına önemli bir etkisi tespit edilemese de, sulandırıcıların dondurma-çözüm sonrası sperm parametrelerini etkilediği ve TES'in epididimal Lewis rat sperması dondurulabilirliğini, önemli derecede geliştirdiği saptandı. Anahtar sözcükler: Dondurma, dondurma hızı, epididimal sperma, sulandırıcı, Lewis rat ırkı.

show abstract

Impact of genomics on research in the rat

Cited by 49 publications

References 137 publications

Proteomic profiling of animal models mimicking skeletal muscle disorders

Proteomic profiling of animal models mimicking skeletal muscle disorders

Chromosome-substituted rat strains provide insights into the genetics of placentation

Influence of extenders and cooling rates on epididymal sperm of Lewis rat strain

Contact Info

Product

Resources

About