Impact of gestational diabetes and lactational insulin replacement on structure and secretory function of offspring rat ventral prostate

Santos, Sérgio A. A.; Rinaldi, Jaqueline de Carvalho; Martins, Amanda E.; Camargo, Ana Carolina Lima; Leonelli, Carina; Delella, Flávia Karina; Felisbino, Sérgio Luís; Justulin, Luís Antônio

doi:10.1016/j.ygcen.2014.06.021

Cited by 12 publications

(15 citation statements)

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“…Sergio et al (2014) studied the effect of insulin on the function and secretion of the prostate of fetuses in maternal diabetic rats. Their finding showed that insulin applied to maternal diabetic rats restored prostate histology and secretion (Santos et al, 2014). Partial maintenance of serum androgens such as testosterone, estradiol, and prolactin in insulin treated rats might be responsible for the maintenance of the histoarchitecture of the ventral prostate as reported by Sudha et al (1999).…”

Section: Kamani M; Mhabadi J A; Atlasi M A; Seyedi F; Kamanmentioning

confidence: 73%

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

et al. 2017

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KAMANI, M.; MHABADI, J. A.; ATLASI, M. A.; SEYEDI, F.; KAMANI, E. & NIKZAD, H.Protective effect of alcoholic extract of garden cress seeds on the histopathological changes of the ventral prostate in streptozotocin diabetic rats. Int. J. Morphol., 35(3):1178Morphol., 35(3): -1184Morphol., 35(3): , 2017. SUMMARY:Diabetes mellitus is a common serious metabolic illness occurring worldwide that may lead to male infertility. Various plants have been used in the treatment of diabetes. In this study, the effect of garden cress (Lepidium sativum) seed extract on fasting blood sugar is assessed for its protective effect on histopathological changes in the ventral prostate gland of streptozotocine-induced diabetic rats. Fifty adult male Wistar rats were randomly selected into five groups. Group 1 was the control placebo group where rats received only 0.1 mL normal saline via gastric gavages. Rats in Group 2 received an intraperitoneal injection of STZ 60 mg/kg body weight and those with FBS >250 mg/dL were considered diabetic. In Group 3, diabetic rats received insulin (3 U/100 g body weight) while in Groups 4 and 5 diabetic rats received 0.1 ml of 200 and 400 mg/kg respectively of an ethanol extract of Lepidium sativum seeds by gavage daily. The prostate was removed and weighed before transfer to Bouin's solution for histological studies. Administration of the 200 and 400 mg/kg doses of Lepidium sativum seed extract increased epithelium height and decreased interstitial volume density and fibromuscular thickness of the prostate significantly. Also, the volume density of the epithelium, fibro muscular, lumen, and interstitial tissues were changed significantly. The results suggest that Lepidium sativum seed extract has beneficial effects as a protective agent against the detrimental effects of diabetes on the reproductive system of diabetic male rats.

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Section: Kamani M; Mhabadi J A; Atlasi M A; Seyedi F; Kamanmentioning

confidence: 73%

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

et al. 2017

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“…In order to analyze whether prolactin interferes with the prostate secretory function in castrated animals, we evaluated the prostatein expression, an androgen-dependent protein secreted by VP (Santos et al 2014). In our study, castration significantly reduced the prostatein expression, whereas the testosterone replacement restored the prostatein synthesis after 10 days of treatment.…”

Section: Discussionmentioning

confidence: 92%

“…The Ki-67 index is expressed as percentages of positive cells from the total cells, counted in five histological sections of five VP lobes per group at 400× (Rinaldi et al 2013;Santos et al 2014). Approximately 8000 cells were counted per experimental group.…”

Section: Determination Of Ki-67 Indexmentioning

confidence: 99%

The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement

et al. 2017

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Despite the androgenic dependence, other hormones, growth factors, and cytokines are necessary to support prostatic growth and maintain the glandular structure; among them, prolactin is a non-steroidal hormone secreted mainly by the pituitary gland. However, extra-pituitary expression of prolactin, such as in the prostate, has also been demonstrated, highlighting the paracrine and autocrine actions of prolactin within the prostate. Here, we investigated whether prolactin modulation alters ventral prostate (VP) morphophysiology in adult castrated rats. Sprague Dawley rats were castrated and after 21 days, divided into ten experimental groups (n = 6/group): castrated control: castrated animals that did not receive treatment; castrated+testosterone: castrated animals that received T (4 mg/kg/day); castrated+PRL (PRL): castrated animals receiving prolactin (0.3 mg/kg/day); castrated+T+PRL: castrated animals that received a combination of testosterone and prolactin; and castrated+bromocriptine (BR): castrated animals that received bromocriptine (0.4 mg/kg/day). The control group included intact animals. The animals were treated for 3 or 10 consecutive days. At the end of experimental period, the animals were euthanized, and the blood and VP lobes were collected and analyzed by different methods. The main findings were that the administration of prolactin to castrated rats did not exert anabolic effects on the VP. Although we observed activation of downstream prolactin signaling after prolactin administration, this was not enough to overcome the prostatic androgen deficiency. Likewise, there was no additional glandular involution in the castrated group treated with bromocriptine. We concluded that despite stimulating the downstream signaling pathway, exogenous prolactin does not act on VP in the absence or presence of high levels of testosterone.

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“…The risk of metabolic, cardiovascular and malignant diseases together with learning disabilities may increase in adult offspring of mothers with intrauterine hyperglycaemia (El Hajj, Schneider, Lehnen, & Haaf, ; Lehnen, Zechner, & Haaf, ). Few recent studies have reported that some reproductive problems such as damaged prostate (Santos et al., ), abnormal sperm DNA methylation (Ge et al., ), decreased sperm count (Amorim et al., ), reduced testosterone, FSH and LH, reduced testis weight, damaged testicular histological structure (Jelodar, Khaksar, & Pourahmadi, ) can be observed in male offspring of diabetic pregnant mice and rats at their adulthood stages. Besides, it has been reported that (Iessi, Sinzato, Gallego, Nielsen, & Damasceno, ) the STZ‐induced GDM causes alterations in circulating levels of insulin, glucagon and somatostatin hormones in both foetus and offspring.…”

Section: Introductionmentioning

confidence: 99%

Effect of gestational diabetes mellitus on testis and pancreatic tissues of male offspring

et al. 2018

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The purpose of this study was to determine the effect of gestational diabetes mellitus (GDM) on some reproductive characteristics, testicular and pancreatic oxidative status and pancreatic endocrine receptor densities of male offspring at post-pubertal stage. A total of 36 1-day-old Wistar Albino male offspring including 12 pups of nontreated mothers (control group), 14 pups of 40 mg/kg STZ-injected mothers (STZ-40 group) and 10 pups of 60 mg/kg STZ-injected mothers (STZ-60 group) were used. The offspring were euthanised on post-natal day 60, their blood, reproductive organs and pancreatic tissues were obtained and examined. When compared with the control group, there was a significant decrease in body and absolute reproductive organ weights, serum testosterone level, testicular and pancreatic catalase activities, pancreatic glutathione level, epididymal sperm concentration of both STZ-40 and STZ-60 groups as well as in testicular glutathione level of only STZ-60 group. Significant increases were determined in testicular and pancreatic malondialdehyde level and glutathione peroxidase activity in both groups and in fasting serum glucose of only STZ-60 group in comparison with the control group. Although some histopathological damages were observed in testes of both STZ-40 and STZ-60 groups, there were no detectable differences between the groups in density of insulin, glucagon and somatostatin receptors in pancreas. In conclusion, GDM has negative effects on reproductive efficiency and testicular-pancreatic tissue oxidant/antioxidant balance of male offspring at post-pubertal stage.

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Impact of gestational diabetes and lactational insulin replacement on structure and secretory function of offspring rat ventral prostate

Cited by 12 publications

References 64 publications

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

Protective Effect of Alcoholic Extract of Garden Cress Seeds on the Histopathological Changes of the Ventral Prostate in Streptozotocin Diabetic Rats

The prostate response to prolactin modulation in adult castrated rats subjected to testosterone replacement

Effect of gestational diabetes mellitus on testis and pancreatic tissues of male offspring

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