Impact of glycosylation on the unimpaired functions of the sperm

Cheon, Yong-Pil; Kim, Chung Hoon

doi:10.5653/cerm.2015.42.3.77

Cited by 25 publications

(19 citation statements)

References 93 publications

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“…Glycosylation, the addition of a sugar moiety to a protein, plays an important role in sperm maturation, function, and fertilisation [ 13 ]. During spermatogenesis, glycosylation ensures the correct distribution of proteins to their structural and functional compartments, and in the epididymis, glycosylation is involved in the maturation of spermatozoa [ 14 ]. After ejaculation, glycoproteins play important roles in the interaction between gametes at the zona pellucida and at the oolemma, ensuring adherence and binding of the two gametes [ 12 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa

Nygaard

Herlihy

Jeanneau

et al. 2018

IJMS

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We question whether the expression of GalNAc-T3, the only known O-GalNAc-transferase present in germ cells, is correlated with qualitative and functional parameters of spermatozoa. We investigated the expression of GalNAc-T3 in ejaculated spermatozoa with immunocytochemistry in swim-up purified and acrosome-reacted spermatozoa from quality-control semen donors and in semen samples from 206 randomly selected men representing a broad spectrum of semen quality. Using donor ejaculates and immunofluorescence detection we found that expression of GalNAc-T3 and the presence of the immature O-glycans Tn and T localized to the equatorial segment of spermatozoa. The proportion of GalNAc-T3-positive spermatozoa in the ejaculate increased after swim-up and appeared unaffected by induction of acrosomal exocytosis. The fraction of spermatozoa with equatorial expression of GalNAc-T3 correlated with classical semen parameters (concentration p = 9 × 10−6, morphology p = 7 × 10−8, and motility p = 1.8 × 10−5) and was significantly lower in men with oligoteratoasthenozoospermia (p = 0.0048). In conclusion, GalNAc-T3 was highly expressed by motile spermatozoa and the expression correlated positively with the classical semen parameters. Therefore, GalNAc-T3 expression seems related to the quality of the spermatozoa, and we propose that reduced expression of GalNAc-T3 may lead to impaired O-glycosylation of proteins and thereby abnormal maturation and reduced functionality of the spermatozoa.

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Section: Introductionmentioning

confidence: 99%

Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa

Nygaard

Herlihy

Jeanneau

et al. 2018

IJMS

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“…CatSper glycoproteins form sperm-specific, voltage-gated Ca 2+ channels localised along the membrane of the sperm flagella. Triggering this channel can increase the probability of sperm–egg binding [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

Duan

et al. 2021

Animals

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To reduce subfertility caused by low semen quality and provide theoretical guidance for the eradication of human male infertility, we sequenced the bovine transcriptomes of round, elongated spermatids and epididymal sperms. The differential analysis was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, Gene Ontology (GO), Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment, protein–protein interaction network (PPI network), and bioinformatics analyses were performed to uncover the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, and glycosylation, and the functional ART3 gene may play important roles during spermiogenesis. Therefore, its localisation in the seminiferous tubules and epididymal sperm were investigated using immunofluorescent analysis, and its structure and function were also predicted. Our findings provide a deeper understanding of the process of spermiogenesis, which involves acrosome formation, histone replacement, and the fine regulation of gene expression.

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“…CatSper glycoproteins form the sperm-specific voltage-gated Ca 2+ channels localized along the membrane of the sperm flagella. The triggering of this channel can increase the probability of sperm-egg binding (11,12). Consequently, epigenetic modification plays an important role in the correct expression of functional genes during spermiogenesis.…”

Section: Introductionmentioning

confidence: 99%

Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

Duan

et al. 2020

Preprint

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To reduce the reproductive loss caused by semen quality and provide theoretical guidance for the eradication of human male infertility, differential analysis of the bovine transcriptome among round spermatids, elongated spermatids, and epididymal sperm was carried out with the reference of the mouse transcriptome, and the homology trends of gene expression to the mouse were also analysed. First, to explore the physiological mechanism of spermiogenesis that profoundly affects semen quality, homological trends of differential genes were compared during spermiogenesis in dairy cattle and mice. Next, the Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, protein-protein interaction network (PPI network), and bioinformatics analysis uncovered the regulation network of acrosome formation during the transition from round to elongated spermatids. In addition, processes that regulate gene expression during spermiogenesis from elongated spermatid to epididymal sperm, such as ubiquitination, acetylation, deacetylation, glycosylation, and the functional gene ART3 may play an important role during spermiogenesis. Therefore, its localisation in the seminiferous tubule was investigated by immunofluorescent analysis, and its structure and function were also predicted. This study provides important data for revealing the mystery of life during spermiogenesis resulting from acrosome formation, histone replacement, and the fine regulation of gene expression.

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Impact of glycosylation on the unimpaired functions of the sperm

Cited by 25 publications

References 93 publications

Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa

Expression of the O-Glycosylation Enzyme GalNAc-T3 in the Equatorial Segment Correlates with the Quality of Spermatozoa

Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

Insights into the Mechanism of Bovine Spermiogenesis Based on Comparative Transcriptomic Studies

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