Impact of granulocyte colony stimulating factor infusion on the implantation rate in women with unexplained previous intracytoplasmic sperm injection failure: a case control study

Abstract

Objective. We studied the G-CSF's effect on women with unexplained infertility and previous ICSI failure. Unexplained infertility is a condition when couples are not able to conceive despite normal semen analysis and absence of female infertility factor(s). Cytokines, transcription factors and signaling pathways are essential for complex interactions of decidualization. G-CSF is a glycoprotein with growth factor and cytokine functions, which are produced in many tissues.Patient and Methods. The study was done on a total of 200 women with only 196 women who ended the study (99 women as a study group, 96 women as a control group). All women were M a n u s c r i p t a c c e p t e d f o r p u b l i c a t i o n complaining of unexplained infertility with previous history of failed ICSI. All cases proceed through ICSI procedure while study group only undergone G-CSF infusion intrauterine at time of ovum pickup.Results. We found that intrauterine G-CSF injection at time of ovum pickup in the study group, in comparison with control group, did not improve neither implantation rate (16.68% vs 19.66%, p = 0.243) nor the chemical (54.5% vs 67%, p = 0.074), clinical pregnancy (51.5% vs 62.9%, p = 0.108) rates as well as live birth rates (31.0% vs 39.8%, p = 0.227). Conclusions.Intrauterine infusion of G-CSF may not improve Implantation rate in women with unexplained previous intracytoplasmic sperm injection (ICSI) failure. Further studies are needed to conclude if the dose, route and timing of the drug administration can give better results.

Platelet-rich plasma (PRP) vs granulocyte colony-stimulating factor (G-CSF) in women with thin endometrium undergoing assisted reproduction: a systematic review and meta-analysis

“…not improve Implantation rate in women with unexplained previous intracytoplasmic sperm injection (ICSI) failure. [28]. However Ismet Hortu et al in their experimental study in rats suggests that G-CSF can be a novel agent for the treatment of ovarian injury.…”