Impact of HBV, HCV and GBV-C/HGV on hepatocellular carcinomas in Europe: results of a European concerted action

Bréchot, Christian; Jaffredo, Franck; Lagorce, David; Gerken, Guido; Büschenfelde, Karl Meyer zum; Papakonstontinou, Anastasia; Hadziyannis, Stephanos J.; Romeo, R.; Colombo, Massimo; Rodés, Joan; Bruix, Jordi; Williams, Roger; Naoumov, Nikolai V.

doi:10.1016/s0168-8278(98)80001-9

Cited by 130 publications

(120 citation statements)

References 57 publications

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“…Thus, our results are not consistent with an association between viral infection and HCC. 16,17,45 We were able to analyze for a large proportion of the study population the presence of both the TTV and GBV-C/ HGV genomes. We found that simultaneous infection by GBV-C/HGV and TTV was rare in the Gabonese population.…”

Section: Discussionmentioning

confidence: 99%

“…[13][14][15] Furthermore, despite several studies carried out in recent years, the role of GBV-C/HGV in pathogenesis is unclear. 16,17 A new viral agent, the TT virus or TTV, has recently been isolated in Japan from the serum of a patient (TT) with posttransfusion hepatitis. 18,19 TT virus (TTV) has a circular singlestranded DNA genome, 3,852 base pairs (bp) in size, the organization of which is similar to that of genomes of the circoviridae.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and genetic variants of hepatitis GB-C/HG and TT viruses in Gabon, equatorial Africa.

Tuveri¹,

Perret²,

Delaporte³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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Abstract. The distribution of Hepatitis GB-C/HG (GB-C/HG) and TT viruses (TTV) infections was investigated in selected populations from Gabon using Polymerase Chain Reaction (PCR) and Enzyme Linked Immunosorbent Assay (ELISA) for anti-Envelop 2 (anti-E2) GBV-C/HGV antibodies. Among pregnant women, 29 of 229 (12.6%) were Hepatitis GB virus-C and Hepatitis G virus (GBV-C/ HGV) RNA positive (ϩ) and 32 of 81 (39.5%) anti-E2 ϩ versus 8 of 39 (20.5%) TTV DNA ϩ. Among sickle cell anemia patients, 9.7% (3/31) were GBV-C/HGV RNA ϩ versus 22.5% (7/31) TTV DNA ϩ. For tuberculosis patients, the figures were 11.5% (4/35) and 0%. A study of hepatocellular carcinoma cases (n ϭ 27) versus controls (n ϭ 66) did not show significant differences for GBV-C/ HGV RNA (10.7% versus 12.1%) and TTV DNA (44.4% versus 30.3%). According to phylogenetic analysis, the 15 GBV-C/HGV strains investigated clustered in group 1, the most common in sub-Saharan Africa whereas TTV sequences (n ϭ 4) mostly clustered in genotypes G1 and one close to genotype G3. In the Gabonese populations investigated, GBV-C/HGV and TTV infections were highly endemic. These data are consistent with the low pathogenicity of these agents.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Prevalence and genetic variants of hepatitis GB-C/HG and TT viruses in Gabon, equatorial Africa.

Tuveri¹,

Perret²,

Delaporte³

et al. 2000

The American Journal of Tropical Medicine and Hygiene

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“…Hepatocellular carcinoma (HCC), the most prevalent histological form, is usually associated with liver cirrhosis and/or chronic viral hepatitis. [1][2][3] Its incidence increases in North American and Western European countries and in Japan. 4,5 Liver transplantation, which allows the treatment of both malignant pathology and hepatic deregulation, is now considered the most satisfying therapeutical approach; however, considering the difficulty to obtain transplantable grafts and the occurrence of relapse, this strategy is restricted to patients exhibiting less than three visible and small-size nodules.…”

mentioning

confidence: 99%

Gene therapy of hepatocarcinoma: a long way from the concept to the therapeutical impact

Gerolami

Uch²,

Bréchot

et al. 2003

Cancer Gene Ther

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“…[1][2][3] Its incidence has increased in Europe, Japan and North America. 4,5 The chemotherapeutical treatment of HCC remains difficult and most of the patients cannot benefit from tumor resection or liver transplantation due to the underlying liver disease or the extension of the tumor at the time of diagnosis.…”

mentioning

confidence: 99%

Hepatoma cell-specific ganciclovir-mediated toxicity of a lentivirally transduced HSV-TkEGFP fusion protein gene placed under the control of rat alpha-fetoprotein gene regulatory sequences

et al. 2003

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Suicide gene therapy combining herpes simplex virus thymidine kinase gene transfer and ganciclovir administration can be envisioned as a powerful therapeutical approach in the treatment of hepatocellular carcinoma; however, safety issues regarding transgene expression in parenchyma cells have to be addressed. In this study, we constructed LATKW, a lentiviral vector expressing the HSV-TkEGFP gene placed under the control of the promoter elements that control the expression of the rat alpha-fetoprotein, and assayed its specific expression in vitro in hepatocarcinoma and nonhepatocarcinoma human cell lines, and in epidermal growth factor stimulated human primary hepatocytes. Using LATKW, a strong expression of the transgene was found in transduced hepatocarcinoma cells compared to a very low expression in nonhepatocarcinoma human cell lines, as assessed by Northern blot, RT-PCR, FACS analysis and ganciclovir-mediated toxicity assay, and no expression was found in lentivirally transduced normal human hepatocytes. Altogether, these results demonstrate the possibility to use a lentivirally transduced expression unit containing the rat alpha-fetoprotein promoter to restrict the HSV-TK-mediated induced GCV sensitivity to human hepatocarcinoma cells.

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Impact of HBV, HCV and GBV-C/HGV on hepatocellular carcinomas in Europe: results of a European concerted action

Cited by 130 publications

References 57 publications

Prevalence and genetic variants of hepatitis GB-C/HG and TT viruses in Gabon, equatorial Africa.

Prevalence and genetic variants of hepatitis GB-C/HG and TT viruses in Gabon, equatorial Africa.

Gene therapy of hepatocarcinoma: a long way from the concept to the therapeutical impact

Hepatoma cell-specific ganciclovir-mediated toxicity of a lentivirally transduced HSV-TkEGFP fusion protein gene placed under the control of rat alpha-fetoprotein gene regulatory sequences

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