Impact of heat and drought stresses on grain nutrient content in chickpea: Genome-wide marker-trait associations for protein, Fe and Zn

Srinivasan, S.; Mahendrakar, Mahesh D.; shankar, Nidhi; Hotti, Avinalappa; Chand, Uttam; Rathore, Abhishek; Gaur, P M

doi:10.1016/j.envexpbot.2021.104688

Cited by 45 publications

(52 citation statements)

References 66 publications

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“…They reported a positive correlation between protein content and micronutrients under temperature and drought stress conditions. However, Fe and Zn were positively correlated under the three treatments, which was also reported in earlier findings in lentil [ 86 ], chickpea [ 87 , 88 ] and pea [ 89 ]. Furthermore, our results showed a significant increase in canopy temperature of plants under high-temperature stress and combined temperature-drought stress.…”

Section: Discussionsupporting

confidence: 88%

High-Temperature and Drought Stress Effects on Growth, Yield and Nutritional Quality with Transpiration Response to Vapor Pressure Deficit in Lentil

Haddad

Choukri

Ghanem

et al. 2021

Plants

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High temperature and water deficit are among the major limitations reducing lentil (Lens culinaris Medik.) yield in many growing regions. In addition, increasing atmospheric vapor pressure deficit (VPD) due to global warming causes a severe challenge by influencing the water balance of the plants, thus also affecting growth and yield. In the present study, we evaluated 20 lentil genotypes under field conditions and controlled environments with the following objectives: (i) to investigate the impact of temperature stress and combined temperature-drought stress on traits related to phenology, grain yield, nutritional quality, and canopy temperature under field conditions, and (ii) to examine the genotypic variability for limited transpiration (TRlim) trait in response to increased VPD under controlled conditions. The field experiment results revealed that high-temperature stress significantly affected all parameters compared to normal conditions. The protein content ranged from 23.4 to 31.9%, while the range of grain zinc and iron content varied from 33.1 to 64.4 and 62.3 to 99.3 mg kg−1, respectively, under normal conditions. The grain protein content, zinc and iron decreased significantly by 15, 14 and 15% under high-temperature stress, respectively. However, the impact was more severe under combined temperature-drought stress with a reduction of 53% in protein content, 18% in zinc and 20% in iron. Grain yield declined significantly by 43% in temperature stress and by 49% in the combined temperature-drought stress. The results from the controlled conditions showed a wide variation in TR among studied lentil genotypes. Nine genotypes displayed TRlim at 2.76 to 3.51 kPa, with the genotypes ILL 7833 and ILL 7835 exhibiting the lowest breakpoint. Genotypes with low breakpoints had the ability to conserve water, allowing it to be used at later stages for increased yield. Our results identified promising genotypes including ILL 7835, ILL 7814 and ILL 4605 (Bakria) that could be of great interest in breeding for high yields, protein and micronutrient contents under high-temperature and drought stress. In addition, it was found that the TRlim trait has the potential to select for increased lentil yields under field water-deficit environments.

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Section: Discussionsupporting

confidence: 88%

High-Temperature and Drought Stress Effects on Growth, Yield and Nutritional Quality with Transpiration Response to Vapor Pressure Deficit in Lentil

Haddad

Choukri

Ghanem

et al. 2021

Plants

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“…As chickpea is consumed in various forms in different ecologies, it serves as an essential nutritional supplement to the poor people living in the drylands of Asia and Africa. Recent studies showed the existence of a large variability for grain protein, Fe and Zn in chickpea germplasm, breeding lines, and cultivars [ 14 , 15 ], which opened ways to understand the genetic architecture and inheritance pattern of the traits for devising breeding strategies. A large variability for grain protein (16.3–26.2%), Fe (44.1–76.7 mg kg −1 ), and Zn (36.3–56.2 mg kg −1 ) contents with moderate-to-high heritability was observed among 280 chickpea accessions used in the current study [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…GWAS was used extensively to characterize the extent of genetic variation and uncover specific genes underlying traits and identification of marker-trait associations for agronomic, biotic, abiotic, and nutritional traits in chickpea [ 17 , 18 , 19 , 20 , 21 , 22 , 23 ] and other legumes such as lentil [ 24 ], soybean [ 25 ], and pea [ 26 ]. In chickpea, MTAs for nutrient traits under normal versus heat- and drought-stress conditions were identified [ 11 , 15 , 27 , 28 , 29 ]; however, these markers need to be validated for use in the marker-assisted selection programs to improve the selection efficiency in the breeding pipeline. In the current study, efforts were also made to compare the MTAs identified in the previous study [ 15 ] to identify more loci-controlling nutrients.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Association Analysis Reveals Trait-Linked Markers for Grain Nutrient and Agronomic Traits in Diverse Set of Chickpea Germplasm

Srungarapu

Mahendrakar

Mohammad

et al. 2022

Cells

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Chickpea is an inexpensive source of protein, minerals, and vitamins to the poor people living in arid and semi-arid regions of Southern Asia and Sub-Saharan Africa. New chickpea cultivars with enhanced levels of protein, Fe and Zn content are a medium-term strategy for supplying essential nutrients for human health and reducing malnutrition. In the current study, a chickpea reference set of 280 accessions, including landraces, breeding lines, and advanced cultivars, was evaluated for grain protein, Fe, Zn content and agronomic traits over two seasons. Using a mid-density 5k SNP array, 4603 highly informative SNPs distributed across the chickpea genome were used for GWAS analysis. Population structure analysis revealed three subpopulations (K = 3). Linkage disequilibrium (LD) was extensive, and LD decay was relatively low. A total of 20 and 46 marker-trait associations (MTAs) were identified for grain nutrient and agronomic traits, respectively, using FarmCPU and BLINK models. Of which seven SNPs for grain protein, twelve for Fe, and one for Zn content were distributed on chromosomes 1, 4, 6, and 7. The marker S4_4477846 on chr4 was found to be co-associated with grain protein over seasons. The markers S1_11613376 and S1_2772537 co-associated with grain Fe content under NSII and pooled seasons and S7_9379786 marker under NSI and pooled seasons. The markers S4_31996956 co-associated with grain Fe and days to maturity. SNP annotation of associated markers were found to be related to gene functions of metal ion binding, transporters, protein kinases, transcription factors, and many more functions involved in plant metabolism along with Fe and protein homeostasis. The identified significant MTAs has potential use in marker-assisted selection for developing nutrient-rich chickpea cultivars after validation in the breeding populations.

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“…Nine significant marker trait associations (MTAs) for SPC were uncovered on LG1, LG2, LG3, LG4, and LG5, explaining 16.85% PV. A recent GWAS using high-throughput SNP markers on 140 chickpea genotypes subjected to drought and heat stress to shed light on MTAs with various nutrients uncovered 66 (non-stress), 46 (drought stress), and 15 (heat stress) MTAs for SPC [ 199 ], which could be used to identify high-protein lines for improving SPC in chickpea.…”

Section: Underpinning Genomic Region/haplotypes Controlling High Prot...mentioning

confidence: 99%

Ensuring Global Food Security by Improving Protein Content in Major Grain Legumes Using Breeding and ‘Omics’ Tools

Jha

Nayyar

Parida

et al. 2022

IJMS

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Grain legumes are a rich source of dietary protein for millions of people globally and thus a key driver for securing global food security. Legume plant-based ‘dietary protein’ biofortification is an economic strategy for alleviating the menace of rising malnutrition-related problems and hidden hunger. Malnutrition from protein deficiency is predominant in human populations with an insufficient daily intake of animal protein/dietary protein due to economic limitations, especially in developing countries. Therefore, enhancing grain legume protein content will help eradicate protein-related malnutrition problems in low-income and underprivileged countries. Here, we review the exploitable genetic variability for grain protein content in various major grain legumes for improving the protein content of high-yielding, low-protein genotypes. We highlight classical genetics-based inheritance of protein content in various legumes and discuss advances in molecular marker technology that have enabled us to underpin various quantitative trait loci controlling seed protein content (SPC) in biparental-based mapping populations and genome-wide association studies. We also review the progress of functional genomics in deciphering the underlying candidate gene(s) controlling SPC in various grain legumes and the role of proteomics and metabolomics in shedding light on the accumulation of various novel proteins and metabolites in high-protein legume genotypes. Lastly, we detail the scope of genomic selection, high-throughput phenotyping, emerging genome editing tools, and speed breeding protocols for enhancing SPC in grain legumes to achieve legume-based dietary protein security and thus reduce the global hunger risk.

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Impact of heat and drought stresses on grain nutrient content in chickpea: Genome-wide marker-trait associations for protein, Fe and Zn

Cited by 45 publications

References 66 publications

High-Temperature and Drought Stress Effects on Growth, Yield and Nutritional Quality with Transpiration Response to Vapor Pressure Deficit in Lentil

High-Temperature and Drought Stress Effects on Growth, Yield and Nutritional Quality with Transpiration Response to Vapor Pressure Deficit in Lentil

Genome-Wide Association Analysis Reveals Trait-Linked Markers for Grain Nutrient and Agronomic Traits in Diverse Set of Chickpea Germplasm

Ensuring Global Food Security by Improving Protein Content in Major Grain Legumes Using Breeding and ‘Omics’ Tools

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