Impact of Hematopoietic Progenitor Cell Count as an Indicator for Optimal Timing of Peripheral Stem Cell Harvest in Clinical Practice

Tanaka, Hiroaki; Ishii, Akihiro; Sugita, Yoshiaki; Shimizu, Ryo; Sato, Fumi; Sakuma, Yukie; Iwai, Rie; Kakuta, Shinichiro

doi:10.3960/jslrt.56.150

Cited by 16 publications

(13 citation statements)

References 16 publications

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“…In contrast, the detection of promyelocytes in peripheral blood is a rapid and low-cost procedure available at any institution that can be followed up by collection on the same day. This would be consistent with previous reports that demonstrated the usefulness of hematopoietic progenitor cells as predictors of the optimal timing of PBSC collection [18,19]. Therefore, promyelocyte detection would seem to be a useful tool when determining the ideal timing of PBSC collection.…”

Section: Discussionsupporting

confidence: 91%

Presence of Promyelocytes in Peripheral Blood as a Novel Predictor of Optimal Timing for Single-Step Peripheral Blood Stem Cell Collection

et al. 2021

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Background: Peripheral blood stem cell (PBSC) collection places a burden on the patient and ideally should be completed in a single procedure. Consequently, a convenient predictive factor is needed for clinical use. Methods: This retrospective study included 72 patients who underwent autologous PBSC collection. A median volume of 3.9×10 6 CD34-positive cells/kg (range: 0.3-47.4×10 6 cells/kg) was collected on the first day. We defined failure as an inability to collect 2.0×10 6 cells/kg on the first day. Patients were classified into failure (n = 25, 34.7%) and success groups (n = 47, 65.3%), and their clinical backgrounds were analyzed. Results: The success group included a significantly larger number of cases in which a differential white blood cell count of the peripheral blood on the day of PBSC collection detected promyelocytes (n = 34, 72.3% vs. failure group: n = 11, 44.0%; P＝0.008). Sixty-two patients underwent autologous PBSC transplantation with a median of 5.6×10 6 transplanted cells/μL (range: 1.60-47.4×10 6 cells/µL). Among transplanted patients, no significant differences were observed between the success and failure groups in terms of the intervals until neutrophil, platelet, and red blood cell engraftment. Conclusion: The presence of promyelocytes in peripheral blood may be a useful indicator of the optimal timing for single-step PBSC collection.

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Section: Discussionsupporting

confidence: 91%

Presence of Promyelocytes in Peripheral Blood as a Novel Predictor of Optimal Timing for Single-Step Peripheral Blood Stem Cell Collection

et al. 2021

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“…The cutoff value of the preharvest CD34+ cell counts was 46.6 × 10 6 /L (SE: 80.2%; SP: 80.8%; PPV: 93.6%; NPV: 53.8%) with an AUC of 0.840. In another study using Sysmex XE2100 and XE5000 analyzers with 22 healthy donors, the optimal cutoff of the HPC count was 44 × 10 6 /L (SE: 69%; SP: 76%; AUC: 0.77).…”

Section: Discussionmentioning

confidence: 98%

“…In the autologous group with this cutoff, the NPV was 100% for both parameters with a PPV of 76.5% for the CD34+ cell count and 68.4% for the HPC count. In a study with 72 patients, 50 autologous and 22 allogeneic, using Sysmex XE2100 and Sysmex XE5000 analyzers, different HPC cutoffs were evaluated . When the HPC count was >100 × 10 6 /L on the day of the harvest, the probability that the apheresis obtained on that day collected >2 × 10 6 /kg CD34+ cells was 70%.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of the predictive value of the hematopoietic progenitor cell count using an automated hematology analyzer for CD34+ stem cell mobilization and apheresis product yield

Furundarena

Uranga

Alkorta

et al. 2019

Int J Lab Hematology

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Introduction We evaluated the value of hematopoietic progenitor cells (HPCs) counted in Sysmex XN analyzers to predict the mobilization and collection of CD34+ cells in apheresis for stem cell transplantation. Methods Eighty patients who underwent stem cell transplantation were enrolled (50 autologous and 30 allogeneic). In the autologous group, patients were considered poor mobilizers when the CD34+ count was <10 × 106/L or <20 × 106/L in patients with multiple myeloma who were going to undergo two transplants. ROC curves were generated, and HPC cutoffs were calculated. Results The correlation between the HPC and CD34+ cell counts was good. Two algorithms were proposed. In the first algorithm, samples collected the day before apheresis, negative and positive HPC cutoffs were selected to detect poor and good mobilization and, therefore, the need or not to administer plerixafor. In the second algorithm, samples collected pre‐apheresis, the negative HPC cutoff was an indication to delay apheresis; an HPC higher than the optimal cutoff was an indication to start apheresis. When the HPC values were between these cutoffs, there was an indication to count CD34+ cells for a better decision‐making. Finally, in samples collected pre‐apheresis, HPC counts could be used to predict patients who would have poor CD34+ cell collections. In the allogeneic group, all the donors mobilized well, and very few needed two apheresis procedures. Conclusions The HPC count is useful for decision‐making in the management of patients subjected to apheresis procedures to collect peripheral blood stem cells.

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“…SCC failure can occur either upfront when patients fail to mobilize and SCC is not even attempted, or when the CD34 + stem cell yield fails to meet the target dose required for transplant(s). Overall, SCC success rates range from 41 to 87% in adult and 83 to 92% in pediatric patients …”

Section: Introductionmentioning

confidence: 99%

Predictive factors for successful peripheral blood stem cell mobilization and collection in children

Truong

Prokopishyn

Luu

et al. 2019

J of Clinical Apheresis

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Factors affecting the success of peripheral blood stem cell collection (SCC) in children are not well characterized. We reviewed 218 stem cell collections among 199 pediatric donors, of which 35 were from healthy sibling donors and 164 were for autologous collections. Successful SCC, defined as a CD34 + cell count of ≥2 × 10 6 /kg of recipient weight per intended transplant, occurred in 188 of 199 donors (94%). Ideal SCC defined ≥5 × 10 6 CD34 + cells/kg of recipient per intended transplant, occurred in 147 (74%) patients. Failure of collection occurred in 11 (6%) patients and was significantly associated with an autologous collection for a brain tumor diagnosis (P = .003) and a pre-apheresis peripheral blood (PB) CD34 + count <20 × 10 6 cells/L (P = .002). Ideal SCC was significantly associated with age < 10 years (P = .01) and preapheresis PB-CD34 + count ≥20 × 10 6 cells/L (P < .0001). Factors associated with failure of SCC may be identified in advance of the collection procedure allowing appropriate counselling of patients as well as anticipatory guidance for multiple collections or justify the preemptive use of stem cell mobilizing agents. K E Y W O R D S apheresis, CD34, donor, risk factors, stem cell collection

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Impact of Hematopoietic Progenitor Cell Count as an Indicator for Optimal Timing of Peripheral Stem Cell Harvest in Clinical Practice

Cited by 16 publications

References 16 publications

Presence of Promyelocytes in Peripheral Blood as a Novel Predictor of Optimal Timing for Single-Step Peripheral Blood Stem Cell Collection

Presence of Promyelocytes in Peripheral Blood as a Novel Predictor of Optimal Timing for Single-Step Peripheral Blood Stem Cell Collection

Evaluation of the predictive value of the hematopoietic progenitor cell count using an automated hematology analyzer for CD34+ stem cell mobilization and apheresis product yield

Predictive factors for successful peripheral blood stem cell mobilization and collection in children

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