Impact of heterogeneously crosslinked calcium alginate networks on the encapsulation of β-carotene-loaded beads

Girón‐Hernández, Joel; Gentile, Piergiorgio; Benlloch-Tinoco, María

doi:10.1016/j.carbpol.2021.118429

Cited by 36 publications

(17 citation statements)

References 26 publications

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“…The sheet does not mention Mannuronic acid/Guluronic acid ratio (M/G) and molecular weight (Mw) of the polymer. However, Girón-Hernández et al reported an alginate with Mw=120,000-190,000 g⋅mol −1 and M/G ratio = 1.56 that its viscosity is the same with the alginate used in this study [16]. as well as the spectra of each material separately (Alg and CMC).…”

Section: Characterization Resultsmentioning

confidence: 61%

A Potential Antibacterial Wound Dressing Based on Cerium Crosslinked Alginate and Carboxymethyl Cellulose

Abtahi

Orhan

Vatansever

et al. 2022

Preprint

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Recently, biopolymer based wound dressing films were studied due to their biocompatibility and biodegradability. In this study, a film was prepared with alginate (Alg) and carboxymethyl cellulose (CMC) by crosslinking with Ce3+ ion to evaluate the antibacterial properties of the film. The film exhibits antibacterial activity against Gram positive (Staphylococcus aureus) and Gram negative (Escherichia coli) bacteria. Physical and chemical characterizations of the film were assessed through FT-IR spectrometry, SEM, swelling, water vapor transmission (WVT), and ultraviolet-visible light transmittance tests. The chemical and physical attributes of the film prove to be potentially advantageous for wound dressings due to its UV-protective, vapor permeable, swellable and antimicrobial properties.

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Section: Characterization Resultsmentioning

confidence: 61%

A Potential Antibacterial Wound Dressing Based on Cerium Crosslinked Alginate and Carboxymethyl Cellulose

Abtahi

Orhan

Vatansever

et al. 2022

Preprint

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“…By the addition of Ca 2+ ions, sodium alginate undergoes the gelation process by interaction of Ca 2+ cations and carboxylate groups of the G‐block regions in the polymer. This binding results in the formation of the junction zones and an array of the cavities like an “egg‐box.” In the presence of Na ion‐containing solutions (e.g., Na‐EDTA, PBS, and sodium citrate) ion exchange and chelate to calcium ions occur that cause dissolution of the calcium alginate gel 53 . The results of degradation tests of A6 and A6P7 scaffolds within 21 days were represented in Figure 9b.…”

Section: Resultsmentioning

confidence: 99%

Development of novel alginate‐polyethyleneimine cell‐laden bioink designed for 3D bioprinting of cutaneous wound healing scaffolds

Khoshnood

Zamanian

2022

J of Applied Polymer Sci

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In this work, the novel bioink composed of alginate and polyethyleneimine were prepared with different concentrations of alginate and polyethyleneimine (PEI) and used to measure printability, rheological behavior, and cellular functions. The results indicated that viscosity tends to increase about 1.3 times with increasing the alginate and PEI content, which gradually affected structural fidelity. The presence of PEI can create a bioink that is partially gelled with higher storage modulus, high shape fidelity, and printability than alginate. Increasing in the mechanical strength and decreasing in the swelling rate for alginate/PEI bioink demonstrated the strong effect of PEI on the enhancing of structure rigidity in the alginate‐based bioinks. Finally, the cell viability and cell proliferation of the 3D alginate/PEI scaffolds were examined using live/dead and 40,6‐diamidino‐2‐phenylindole staining to select the best bioink formulation. Fibroblast‐laden bioink of 6% alginate and 7% PEI indicated the high proliferation of the printed cells via increasing in hydrophilicity of the surface. The in‐vivo evaluations on the wound rat model indicated that the 3D scaffold effectively accelerated the wound healing process and promoted tissue regeneration by activation of vimentin along with cytokeratin as well as collagen synthesis. Hair follicles started to form at Day 7 in alginate/PEI scaffold.

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“…Once droplets have reached the bath, ion diffusion occurs by a promotion of physical bonds among the guluronic fraction of the polymer chains, with the formation of not homogeneous regions until the formation of microgels. While alginate tends to instantaneously form gel-like phases onto the surface, a gradient variously crosslinked zones is formed due to the different ion extent, related to the diffusion time from the core towards the gelled zone [ 27 ]. In the case of Alg/Gel microgels, polar groups of protein tend to influence the ion diffusion mechanism through the alginate network, thus contributing to reducing structural heterogeneity, also with effects on the diameter distribution.…”

Section: Resultsmentioning

confidence: 99%

3D Scaffolds Fabrication via Bicomponent Microgels Assembly: Process Optimization and In Vitro Characterization

Cruz‐Maya

Guarino

2022

Micromachines

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In the last decade, different technological approaches have been proposed for the fabrication of 3D models suitable to evaluate in vitro cell response. Among them, electro fluid dynamic atomization (EFDA) belonging to the family of electro-assisted technologies allows for the dropping of polysaccharides and/or proteins solutions to produce micro-scaled hydrogels or microgels with the peculiar features of hydrogel-like materials (i.e., biocompatibility, wettability, swelling). In this work, a method to fabricate 3D scaffolds by the assembly of bicomponent microgels made of sodium alginate and gelatin was proposed. As first step, optical and scanning electron microscopy with the support of image analysis enabled to explore the basic properties of single blocks in terms of correlation between particle morphology and process parameters (i.e., voltage, flow rate, electrode gap, and needle diameter). Chemical analysis via ninhydrin essays and FTIR analysis confirmed the presence of gelatin, mostly retained by physical interactions into the alginate network mediated by electrostatic forces. In vitro tests confirmed the effect of biochemical signals exerted by the protein on the biological response of hMSCs cultured onto the microgels surface. Hence, it is concluded that alginate/gelatin microgels assemblies can efficiently work as 3D scaffolds able to support in vitro cells functions, thus providing a friendly microenvironment to investigate in vitro cell interactions.

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Impact of heterogeneously crosslinked calcium alginate networks on the encapsulation of β-carotene-loaded beads

Cited by 36 publications

References 26 publications

A Potential Antibacterial Wound Dressing Based on Cerium Crosslinked Alginate and Carboxymethyl Cellulose

A Potential Antibacterial Wound Dressing Based on Cerium Crosslinked Alginate and Carboxymethyl Cellulose

Development of novel alginate‐polyethyleneimine cell‐laden bioink designed for 3D bioprinting of cutaneous wound healing scaffolds

3D Scaffolds Fabrication via Bicomponent Microgels Assembly: Process Optimization and In Vitro Characterization

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