Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.)

Estruch, Guillem; Martínez‐Llorens, Silvia; Tomás‐Vidal, Ana; Monge-Ortiz, Raquel; Jover‐Cerdá, Miguel; Brown, Paul B.; Peñaranda, David S.

doi:10.1016/j.jprot.2020.103672

Cited by 23 publications

(20 citation statements)

References 98 publications

(133 reference statements)

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“…In phase II (up to 250 g), there were no signi cant differences between experimental groups (FM and PP), thus supporting that older sh are more tolerant to diet plant proteins than sh in earlier growth stages [16]. It is noteworthy the lack of in ammatory response in PP* group (short PP exposure), supporting that PP long term feeding has deeper alterations in sh gut and contributes to changes in the microbiota and an increase of sh mortality [32], in agreement with previous studies [33,67,68],…”

Section: Discussionsupporting

confidence: 89%

“…In so far as de cient diets could be considered a stress factor, long term feeding could determine suppressive or depressive effects on the immune mechanisms [24,28,32,33]. The down-regulation of mRNA expression of some immune related genes with the increased plant proteins in the diet has also been reported in other species [69].…”

Section: Discussionmentioning

confidence: 95%

“…Previous studies in seabream demonstrated that the use of plant proteins induced signi cant alterations of the gut microbiota, gut gene expression and gut proteomic pro le [32,42,43]. Nonetheless, these alterations can be affected to different extent by the feeding period or sh stage.…”

Section: Discussionmentioning

confidence: 99%

“…Fish, rearing system conditions, diets and feeding conditions A total number of 240 juveniles of gilthead seabream (averagare weigth 7,5 g and 60 days) were obtained from the sh farm BERSOLAZ (Bersolaz Spain, S.L.U, Culmarex Group) located in Port de Sagunt (Valencia, Spain) and transported to the facilities at the Universitat Politècnica de València, where the growth trial was conducted after 15 days of adaptation to experimental conditions. Features of the system and water parameters set were described in previous growth trials carried out in these facilities [32,33]. Lighting conditions were determined by the natural photoperiod.…”

Section: Ethics Statementmentioning

confidence: 99%

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Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Peñaranda

Bäuerl

Tomás‐Vidal

et al. 2020

Preprint

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BackgroundAlthough high levels of fish meal replacement by alternative protein sources have been achieved without relevant alterations in terms of growth performance, negative effects on immune status were detected. The diet and fish immunity interactions at gut level have been widely discussed, although in vivo approaches have reported several limitations. In this sense, intestine explant culture system can be a valuable complementary tool to study the interactions between pathogenic bacteria and fish gut response, and the possible influence of environmental, breeding, rearing conditions or dietary components on the responsiveness of the innate immune system in fish.The object of this study was to test the impact of total substitution of fish meal by plant protein on the intestinal health of seabream (12g) in two growth stages: phase I (90 days), up to 68 g, and phase II (305 days), up to 250g. In phase II, the effects of the long term and short exposure (15 days) to plant protein diet were determined. In order to determine the effect of plant protein feeding on the innate immune response to bacterial pathogens, and an ex vivo procedure of intestine explants culture was implemented.ResultsFish showed less tolerance to dietary plant protein in phase I than in phase II, while the ex vivo assays indicated that the intestine from fish fed at short-term plant diets showed a higher immune response than at long term feeding.In relation to the immune response to bacterial challenge, a significant expression in pro-inflammatory cytokines IL-1β and IL-6 after 6 hours of exposure to V. algynoliticus, while COX-2 expression was significantly induced by P. damselae subsp. pisicida, showing positive high correlation between them.ConclusionsA differential health status was observed depending of growth stage, being stricter to the plant protein inclusion the younger fish. The new experimental system based on fish intestinal explants culture has been successfully implemented, becoming an effective methodology for ex vivo studies. Under ex vivo conditions, the bacterial challenge induced inflammatory and immune intestinal response, responding stronger those intestine of fish fed during a short-term with a total substitution of fish meal.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Section: Ethics Statementmentioning

confidence: 99%

See 2 more Smart Citations

Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Peñaranda

Bäuerl

Tomás‐Vidal

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The findings of the present study suggest that the fish gut response to the total dietary substitution of fish meal by plant protein meals differs between short- and long-term feeding and the fish size. In addition, previous studies in vivo carried out with seabream demonstrated that the use of plant proteins induced significant alterations of the gut microbiota, gut gene expression, and gut proteomic profile [ 33 , 34 , 35 ]. Nonetheless, these alterations can be affected to a different extent by the feeding period or fish stage.…”

Section: Discussionmentioning

confidence: 99%

Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Peñaranda

Bäuerl

Tomás‐Vidal

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The interaction between diet and intestinal health has been widely discussed, although in vivo approaches have reported limitations. The intestine explant culture system developed provides an advantage since it reduces the number of experimental fish and increases the time of incubation compared to similar methods, becoming a valuable tool in the study of the interactions between pathogenic bacteria, rearing conditions, or dietary components and fish gut immune response. The objective of this study was to determine the influence of the total substitution of fish meal by plants on the immune intestinal status of seabream using an ex vivo bacterial challenge. For this aim, two growth stages of fish were assayed (12 g): phase I (90 days), up to 68 g, and phase II (305 days), up to 250 g. Additionally, in phase II, the effects of long term and short term exposure (15 days) to a plant protein (PP) diet were determined. PP diet altered the mucosal immune homeostasis, the younger fish being more sensitive, and the intestine from fish fed short-term plant diets showed a higher immune response than with long-term feeding. Vibrio alginolyticus (V. alginolyticus) triggered the highest immune and inflammatory response, while COX-2 expression was significantly induced by Photobacterium damselae subsp. Piscicida (P. damselae subsp. Piscicida), showing a positive high correlation between the pro-inflammatory genes encoding interleukin 1β (IL1-β), interleukin 6 (IL-6) and cyclooxygenase 2(COX-2).

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Soluble non-starch polysaccharides in fish feed: implications for fish metabolism

Wang

Zou

2022

Fish Physiol Biochem

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Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.)

Abstract: Impact of high dietary plant protein with or without marine ingredients in gut mucosa proteome of gilthead seabream (Sparus aurata, L.

Cited by 23 publications

References 98 publications

Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Intestinal Explant Cultures from Gilthead Seabream (Sparus Aurata, L.) Allowed Determining the Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Intestinal Explant Cultures from Gilthead Seabream (Sparus aurata, L.) Allowed the Determination of Mucosal Sensitivity to Bacterial Pathogens and the Impact of a Plant Protein Diet

Soluble non-starch polysaccharides in fish feed: implications for fish metabolism

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