Impact of high hydrostatic pressure on bacterial proteostasis

Gayán, Elisa; Govers, Sander K.; Aertsen, Abram

doi:10.1016/j.bpc.2017.03.005

Cited by 38 publications

(33 citation statements)

References 99 publications

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“…The results of the API strip analysis conducted here provide only very limited information on the enzymatic activity spectra and are not reflective for their intensities under deep-sea conditions, such as low temperatures and high pressure. For instance, exposure to higher hydrostatic pressure (>100 bar) might limit microbial growth, disrupt protein homeostasis, and conformational change in ribosomes structure (Gayán et al, 2017). Reaching environmental conditions for experimental in vitro study is crucial but rarely feasible in the case of deepsea conditions (e.g., the hydrostatic pressure of min 400 bar).…”

Section: Discussionmentioning

confidence: 99%

Heavy-Metal-Resistant Microorganisms in Deep-Sea Sediments Disturbed by Mining Activity: An Application Toward the Development of Experimental in vitro Systems

et al. 2019

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Future mining of polymetallic nodules in the Clarion Clipperton Zone (Northeastern Pacific) is expected to affect all benthic ecosystems. The diversity, distribution, and environmental functions of microorganisms inhabiting abyssal sediments are barely understood. To understand consequences of deep-sea mining, experimental in vitro systems needs to be established to test hypotheses on the environmental impact of mining. For this, 40 bacterial strains, belonging to proteobacteria, actinobacteria and firmicutes were isolated from deep-sea sediments and nodules sampled at depths of ≥ 4000 m. Phenotypic characterization revealed a strong inter-species and moderate intra-species variability. Determination of metal minimum inhibitory concentrations indicated the presence of acute manganese-resistant bacteria such as Rhodococcus erythropolis (228.9 mM), Loktanella cinnabarina (57.2 mM), and Dietzia maris (14.3 mM) that might be suitable systems for testing the effects of release of microbes from nodules and their interactions with sediment particles in plumes generated during mining. Comparative genomic analysis indicated the presence of manganese efflux systems relevant for future transcriptomics or proteomics approaches with environmental samples and might serve in paving the way to develop model systems including representative organisms which are currently not cultivable. Monitoring deep-sea mining activity at abyssal depth is a challenge that has to be tackled. We proposed the use of API strips as a fast on-board methodology for bacterial monitoring as an indicator for sediment plume dispersions within the water column.

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Section: Discussionmentioning

confidence: 99%

Heavy-Metal-Resistant Microorganisms in Deep-Sea Sediments Disturbed by Mining Activity: An Application Toward the Development of Experimental in vitro Systems

et al. 2019

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“…To further explore how inclusion bodies change under stress, the proteomes of E. coli MG1655 ibpA-yfp(pRK767) and MG1655 ibpA-yfp(pLHR) whole cell extracts and inclusion bodies were analyzed after pressure treatment at 400 MPa (Figure 4). Pressure treatment disrupts protein aggregates and bacterial membranes (Gänzle and Liu, 2015;Gayán et al, 2017). In untreated cells, proteome analysis identified proteins that were enriched in the whole cell extract as well as proteins that were enriched in the inclusion bodies (Figures 4A,B).…”

Section: Effect Of Pressure On the Proteome Of E Colimentioning

confidence: 99%

Heat and Pressure Resistance in Escherichia coli Relates to Protein Folding and Aggregation

Mercer

Behr

et al. 2020

Front. Microbiol.

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The locus of heat resistance (LHR) confers extreme heat resistance in Escherichia coli. This study explored the role of the LHR in heat and pressure resistance of E. coli, as well as its relationship with protein folding and aggregation in vivo. The role of LHR was investigated in E. coli MG1655 and the pressure resistant E. coli LMM1010 expressing an ibpA-yfp fusion protein to visualize inclusion bodies by fluorescence microscopy. The expression of proteins by the LHR was determined by proteomic analysis; inclusion bodies of untreated and treated cells were also analyzed by proteomics, and by fluorescent microscopy. In total, 11 proteins of LHR were expressed: sHSP20, ClpK GI , sHSP, YdfX1 and YdfX2, HdeD, KefB, Trx, PsiE, DegP, and a hypothetical protein. The proteomic analysis of inclusion bodies revealed a differential abundance of proteins related to oxidative stress in strains carrying the LHR. The LHR reduced the presence of inclusion bodies after heat or pressure treatment, indicating that proteins expressed by the LHR prevent protein aggregation, or disaggregate proteins. This phenotype of the LHR was also conferred by expression of a fragment containing only sHSP20, ClpK GI , and sHSP. The LHR and the fragment encoding only sHSP20, ClpK GI , and sHSP also enhanced pressure resistance in E. coli MG1655 but had no effect on pressure resistance of E. coli LMM1010. In conclusion, the LHR confers pressure resistance to some strains of E. coli, and reduces protein aggregation. Pressure and heat resistance are also dependent on additional LHR-encoded functions.

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“…High hydrostatic pressure treatment (HHP) is the most established non-thermal technology in industrial food production and capable of the successful reduction of vegetative pathogenic and spoilage bacteria, with a minimal degradation of valuable food constituents ( Gayan et al, 2017 ).…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

“…In general, the effect of HHP on microorganisms is based on the principle of Le Châtelier and Braun, i.e., the external force applied on a product in the form of an increased pressure causes a shift of the thermodynamic equilibrium in such a way that (biochemical) molecules are reduced in volume ( Gayan et al, 2017 ). Furthermore, the principle of microscopic ordering states that under HHP conditions, the degree of ordering of molecules is increased, further changing the thermodynamic behavior of molecules, such as melting temperature ( Balny and Masson, 1993 ).…”

Section: Overview Of Non-thermal Inactivation Technologiesmentioning

confidence: 99%

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

et al. 2018

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The viable but non-culturable (VBNC) state, as well as sublethal injury of microorganisms pose a distinct threat to food safety, as the use of traditional, culture-based microbiological analyses might lead to an underestimation or a misinterpretation of the product’s microbial status and recovery phenomena of microorganisms may occur. For thermal treatments, a large amount of data and experience is available and processes are designed accordingly. In case of innovative inactivation treatments, however, there are still several open points with relevance for the investigation of inactivation mechanisms as well as for the application and validation of the preservation processes. Thus, this paper presents a comprehensive compilation of non-thermal preservation technologies, i.e., high hydrostatic pressure (HHP), pulsed electric fields (PEFs), pulsed light (PL), and ultraviolet (UV) radiation, as well as cold plasma (CP) treatments. The basic technological principles and the cellular and molecular mechanisms of action are described. Based on this, appropriate analytical methods are outlined, i.e., direct viable count, staining, and molecular biological methods, in order to enable the differentiation between viable and dead cells, as well as the possible occurrence of an intermediate state. Finally, further research needs are outlined.

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Impact of high hydrostatic pressure on bacterial proteostasis

Cited by 38 publications

References 99 publications

Heavy-Metal-Resistant Microorganisms in Deep-Sea Sediments Disturbed by Mining Activity: An Application Toward the Development of Experimental in vitro Systems

Heavy-Metal-Resistant Microorganisms in Deep-Sea Sediments Disturbed by Mining Activity: An Application Toward the Development of Experimental in vitro Systems

Heat and Pressure Resistance in Escherichia coli Relates to Protein Folding and Aggregation

Sublethal Injury and Viable but Non-culturable (VBNC) State in Microorganisms During Preservation of Food and Biological Materials by Non-thermal Processes

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