Impact of high magnification sperm selection on neonatal outcomes: a retrospective study

Gaspard, Olivier; Vanderzwalmen, Pierre; Wirleitner, Barbara; Ravet, Stéphanie; Wenders, Frédéric; Eichel, Verena; Mocková, Alice; Spitzer, D; Jouan, Caroline; Gridelet, Virginie; Martens, Henri; Henry, Laurie; Zech, Herbert; d'HAUTERIVE, Sophie Perrier; Nisolle, Michelle

doi:10.1007/s10815-018-1167-8

Cited by 12 publications

(7 citation statements)

References 59 publications

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“…It was also reported that ICSI performed with spermatozoa selected for better morphology at a high magnification (> 6000×) can achieve better clinical outcomes than conventional ICSI [30,31]. Some studies found that compared with conventional ICSI, intracytoplasmic morphologically selected sperm injection (IMSI) can decrease the risk of birth defects in newborns [32][33][34]. On the other hand, this decrease may also be related to the semen processing method.…”

Section: Discussionmentioning

confidence: 99%

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

et al. 2020

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Background: Most studies have mainly focused on the effects of the sperm DNA fragmentation index (DFI) on fertilization, embryonic developmental potential and aneuploidy, pregnancy and abortion rates after in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) and have remained controversial. However, few studies have reported the effects of sperm DFI on neonatal outcomes, including stillbirths, neonatal deaths, sex, gestational age, prematurity, birthweight, low birth weight (LBW) and birth defects in newborns. Our objective was to evaluate the effects of sperm DFI on the clinical and neonatal outcomes of ICSI cycles. Methods: This retrospective study analysed a total of 2067 oocyte retrieval, 1139 transfer and 713 delivery cycles from conventional ICSI cycles, including 301, 469, and 214 live-born infants in groups segregated according to sperm DFI as the < 15%, 15-30% and > 30% groups, respectively. The clinical and neonatal outcomes were compared among the three groups. Results: Sperm DFI did not significantly affect the rates of fertilization, clinical pregnancy, miscarriage or ongoing pregnancy. Sperm DFI did not increase the risk of stillbirths or neonatal deaths. The rates of stillbirths and neonatal deaths were not significantly different among the three groups. The sex, gestational age, prematurity, birthweight and LBW of newborns in the three groups were not significantly affected by sperm DFI. Moreover, sperm DFI did not increase the number of birth defects in children. Conclusions: Sperm DFI did not affect the clinical or neonatal outcomes of ICSI cycles.

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Section: Discussionmentioning

confidence: 99%

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

et al. 2020

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“…Morphological abnormalities of the human sperm head have been shown to be associated with different types of genetic abnormalities [79], increased sperm DNA fragmentation [80] and different potentially harmful epigenetic factors, such as abnormal patterns of DNA methylation [81] and the absence or defective function of the sperm-derived oocyte-activating factor [82]. These observations explain the findings of increased implantation and pregnancy rate and decreased miscarriage rate [83][84][85], as well as a significantly decreased risk of major birth defects [86,87], with the use of high-magnification ICSI (IMSI) as compared with conventional ICSI, although some studies failed to confirm these differences [88,89].…”

Section: Current Clinical Experiencementioning

confidence: 99%

Paternal Effects on Embryonic, Fetal and Offspring Health: The Role of Epigenetics in the ICSI and ROSI Era

Tesarik

2020

Innovations in Assisted Reproduction Technology

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Paternal effects on the developmental potential of human embryos have been studied since the early 1990s, particularly with respect to newly emerging assisted reproduction technologies. Both genetic and epigenetic paternal effects can influence postfertilization development and cause implantation failure or miscarriage. However, it is only over the last few years that issues related to paternal effects associated with different assisted reproduction techniques on the health status of newborn and adult progeny have been focused. At the same time, new findings point out different, yet unexplored, areas of research into the potentially responsible factors, including the activity of the sperm-derived oocyte-activating factor and the oocyte signaling pathways mediating its action, the methylation status of both imprinted and non-imprinted genes, correct replacement of sperm nuclear protamines with oocyte-derived histones, the histone acetylation status, and the function of sperm-borne small RNAs. It is increasingly important to know how these developmentally important epigenetic regulators can be altered in the context of the current micromanipulation-assisted fertilization techniques, intracytoplasmic sperm injection (ICSI) and round spermatid injection (ROSI). Last but not least, transgenerational transmission of acquired, environmentally conditioned disorders from fathers to offspring is a newly emerging issue which warrants further research.

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“…Moreover, a meta-analysis suggests an increased risk of birth defects in children conceived by ICSI compared with those born after in vitro fertilization (IVF) or spontaneous conception [13]. However, the rate of major malformations is significantly reduced when ICSI is performed after sperm selection at very high magnification [14,15], highlighting the importance of sperm selection. In addition, the approaches currently used for sperm selection are still not fully adequate [16], emphasizing the need of a strategy that takes into account not only morphological features but also functional.…”

Section: Introductionmentioning

confidence: 99%

Molecular Profiling of Spermatozoa Reveals Correlations between Morphology and Gene Expression: A Novel Biomarker Panel for Male Infertility

Cassuto¹,

Piquemal

Boitrelle

et al. 2021

BioMed Research International

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Choosing spermatozoa with an optimum fertilizing potential is one of the major challenges in assisted reproductive technologies (ART). This selection is mainly based on semen parameters, but the addition of molecular approaches could allow a more functional evaluation. To this aim, we used sixteen fresh sperm samples from patients undergoing ART for male infertility and classified them in the high- and poor-quality groups, on the basis of their morphology at high magnification. Then, using a DNA sequencing method, we analyzed the spermatozoa methylome to identify genes that were differentially methylated. By Gene Ontology and protein–protein interaction network analyses, we defined candidate genes mainly implicated in cell motility, calcium reabsorption, and signaling pathways as well as transmembrane transport. RT-qPCR of high- and poor-quality sperm samples allowed showing that the expression of some genes, such as AURKA, HDAC4, CFAP46, SPATA18, CACNA1C, CACNA1H, CARHSP1, CCDC60, DNAH2, and CDC88B, have different expression levels according to sperm morphology. In conclusion, the present study shows a strong correlation between morphology and gene expression in the spermatozoa and provides a biomarker panel for sperm analysis during ART and a new tool to explore male infertility.

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Impact of high magnification sperm selection on neonatal outcomes: a retrospective study

Cited by 12 publications

References 59 publications

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

Effects of the sperm DNA fragmentation index on the clinical and neonatal outcomes of intracytoplasmic sperm injection cycles

Paternal Effects on Embryonic, Fetal and Offspring Health: The Role of Epigenetics in the ICSI and ROSI Era

Molecular Profiling of Spermatozoa Reveals Correlations between Morphology and Gene Expression: A Novel Biomarker Panel for Male Infertility

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