Impact of Histidine rich glycoprotein gene polymorphism in infertile male on the outcome of intracytoplasmic sperm injection (ICSI)

Abstract

Introduction: Infertility is a major concern among married couples when they fail to achieve conception.The presence of genetic anomalies both at chromosomal and gen level is a major concern in the couples opting for assisted reproductive techniques (ARTs). Male infertility may be related to various genetic polymorphisms and their combinations and not solely on a single one. Perhaps in the future, a panel of polymorphisms may guide the clinicians and patients to the best treatment alternatives of infertility. Study design: This is a cohort prospective study including 100 infertile male (oligozoospermia) to determine the impact of Histidine rich glycoprotein gene polymorphism HRG (C633T) in infertile male on the outcome of intra cytoplasmic sperm injection (ICSI). Results: In this study 56, 52 % of infertile males with successed ICSI have C/T genotype, 34.78% of them have C/C genotype and 8.69% of them have T/T genotype. While 33.33% of infertile male with failed ICSI have C/T genotype, 37.03 % of them have C/C genotype and 29.62 % of them have T/T genotype. There was significant increase of C/T mutation (56.52%) in infertile males with successful ICSI compared to failed ICSI (33.33%) p˂0.05. There was significant decrease of T/T mutation (8.69%) in infertile males with successful ICSI compared to failed ICSI (29.26%) p˂0.05. There were significant differences between HRG C/Tgenotype compared to C/C and T/T genotypes regarding sperm count, sperm concentration and total motility p˂0.05.