Impact of hormone replacement on myocardial fatty acid metabolism: Potential role of estrogen

Herrero, Pilar; Soto, Pablo; Dence, Carmen S.; Kisrieva-Ware, Zulfia; Delano, Deborah A.; Peterson, Linda R.; Gropler, Robert J.

doi:10.1016/j.nuclcard.2005.05.009

Cited by 39 publications

(24 citation statements)

References 37 publications

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“…As a result, cellular ATP levels were reduced and the activation status of AMP-Kα, which can regulate that activity of Runx2 (21), was increased. Estrogen favors fatty acid metabolism in a number of tissues (39)(40)(41)(42) and may therefore prevent the switch to glycolytic metabolism in osteoblasts. This idea is worthy of additional study and will likely require the use of models in which estrogen receptor signaling has been modified in the osteoblast lineage.…”

Section: Discussionmentioning

confidence: 99%

“…We speculated that the more severe defect in in vitro maturation induced by E2 treatment of ΔCpt2 osteoblast might be due to an inability to adjust fuel selection, since E2 favors fatty acid utilization in a number of tissues (39)(40)(41)(42). To test this idea, control and ΔCpt2 osteoblast cultured in the presence or absence of E2 were labeled with 2-deoxy-D-[ Figure 3G), suggestive of an adjustment in cellular metabolism to maintain ATP levels.…”

Section: Skeletal Accumulation Of Fatty Acidsmentioning

confidence: 99%

See 1 more Smart Citation

Fatty acid oxidation by the osteoblast is required for normal bone acquisition in a sex- and diet-dependent manner

Kim¹,

Li²,

Zoch³

et al. 2017

JCI Insight

106

101

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Section: Discussionmentioning

confidence: 99%

Section: Skeletal Accumulation Of Fatty Acidsmentioning

confidence: 99%

Fatty acid oxidation by the osteoblast is required for normal bone acquisition in a sex- and diet-dependent manner

Kim¹,

Li²,

Zoch³

et al. 2017

JCI Insight

106

101

View full text Add to dashboard Cite

“…We presumed that estrogen had a key modulator role in lipid metabolism. Herrero et al reported that myocardial fatty acid utilization was higher in women taking estrogens compared to those not receiving estrogens, which might be attributable to the regulation of β-oxidation of fatty acids [39] . It has been reported that mRNA expression and the activity of enzymes including medium-chain acyl coenzyme A dehydrogenase and acetyl CoA oxidase, enzymes with fatty acid β-oxidation, are reduced under estrogen deficiency [40] .…”

Section: Discussionmentioning

confidence: 99%

GC-TOF/MS-based metabolomic profiling of estrogen deficiency-induced obesity in ovariectomized rats

Zhang

Wang

et al. 2011

Acta Pharmacol Sin

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Aim: To explore the alteration of endogenous metabolites and identify potential biomarkers using metabolomic profiling with gas chromatography coupled a time-of-flight mass analyzer (GC/TOF-MS) in a rat model of estrogen-deficiency-induced obesity. Methods: Twelve female Sprague-Dawley rats six month of age were either sham-operated or ovariectomized (OVX). Rat blood was collected, and serum was analyzed for biomarkers using standard colorimetric methods with commercial assay kits and a metabolomic approach with GC/TOF-MS. The data were analyzed using multivariate statistical techniques. Results: A high body weight and body mass index inversely correlated with serum estradiol (E2) in the OVX rats compared to the sham rats. Estrogen deficiency also significantly increased serum total cholesterol, triglycerides, and low-density lipoprotein cholesterol. Utilizing GC/TOF-MS-based metabolomic analysis and the partial least-squares discriminant analysis, the OVX samples were discriminated from the shams. Elevated levels of cholesterol, glycerol, glucose, arachidonic acid, glutamic acid, glycine, and cystine and reduced alanine levels were observed. Serum glucose metabolism, energy metabolism, lipid metabolism, and amino acid metabolism were involved in estrogen-deficiency-induced obesity in OVX rats. Conclusion: The series of potential biomarkers identified in the present study provided fingerprints of rat metabolomic changes during obesity and an overview of multiple metabolic pathways during the progression of obesity involving glucose metabolism, lipid metabolism, and amino acid metabolism.

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“…This gender-related difference may be a result of the fact that, in hearts from females, glucose metabolism may be decreased during ischemia because of substrate competition, as a result of a greater fatty acid delivery to the myocardium. A trend towards higher myocardial use of fatty acids was noted retrospectively in women taking estrogens when compared with untreated women (22).…”

Section: Discussionmentioning

confidence: 93%

“…This would be consistent with a greater depletion of glycogen stores in hearts from females relative to males when preserved in this cardioplegic solution. Female hearts are characterized by a greater myocardial blood flow (22,23), which leads to higher cardiac work and oxygen consumption, lower efficiency and lower fasting myocardial glucose utilization. Greater myocardial blood flow also tends to be associated with greater myocardial fatty acid utilization and oxidation (24,25).…”

Section: Discussionmentioning

confidence: 99%

Substrate selection in hearts subjected to ischemia/reperfusion: role of cardioplegic solutions and gender

2011

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In conditions of ischemia/reperfusion (I/R), the relative use of all available substrates by the heart has a significant effect on the recovery of the organ. This substrate preference in perfused hearts is influenced by ischemia. We followed the metabolic fate of [U-(13) C]glucose and [3-(13) C]lactate in hearts preserved in Celsior (Cs) and histidine buffer solution (HBS) for 4 or 6 h and subsequently perfused with a Krebs-Henseleit solution (KH) containing [U-(13) C]glucose and [3-(13) C]lactate. We also assessed gender-specific metabolic modulation in our I/R experimental conditions. Hearts from male and female Wistar rats (6-8 weeks) were subjected to moderate (0-240 min) or prolonged (240-360 min) cold ischemia whilst immersed in Cs and HBS, and perfused for 30 min with KH containing [U-(13) C]glucose and [3-(13) C]lactate. After perfusion, hearts were freeze-clamped and metabolites were extracted for (13) C NMR isotopomer analysis. In control conditions, there were no differences with regard to lactate origin in hearts from males and females. After 6 h of preservation in Cs, lactate origin was mostly from [U-(13) C]glucose in hearts from males and from [3-(13) C]lactate in hearts from females. During the 6 h of organ preservation in HBS, the lactate pool showed a strong contribution from unenriched sources in male hearts and from [U-(13) C]glucose in female hearts. The glutamate C2/C4 ratio was stable or increased in hearts from females after I/R, and the alanine index increased in hearts from both males and females. Octanoate was, as predicted, the preferential substrate during perfusion. Glucose and lactate suffer a distinct metabolic fate in our I/R conditions, which is related to the cardioplegic solution used during organ storage, and the gender. Hearts from females appear to be less sensitive to I/R injury, and heart preservation in HBS proved to be effective in enhancing anaplerosis during perfusion, especially in hearts from females.

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Impact of hormone replacement on myocardial fatty acid metabolism: Potential role of estrogen

Abstract: In postmenopausal women, estrogen use is associated with increased myocardial fatty acid utilization. Thus, when the cardiac effects of hormone replacement therapy are being assessed, alterations in myocardial substrate metabolism should be considered.

Cited by 39 publications

References 37 publications

Fatty acid oxidation by the osteoblast is required for normal bone acquisition in a sex- and diet-dependent manner

Fatty acid oxidation by the osteoblast is required for normal bone acquisition in a sex- and diet-dependent manner

GC-TOF/MS-based metabolomic profiling of estrogen deficiency-induced obesity in ovariectomized rats

Substrate selection in hearts subjected to ischemia/reperfusion: role of cardioplegic solutions and gender

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