Impact of Hyaluronic acid on healing of tongue ulcers in diabetic rats

Abstract

Introduction: Wound healing is a highly complicated process achieved through definitely programmed stages. Hyaluronic acid is a natural component present essentially in the skin and other connective tissues, it has been used in many medical fields for acceleration of wound healing.Aim: This study aimed to investigate the therapeutic effect of hyaluronic acid on healing of tongue ulcer in diabetic rat model. Material & Methods:Thirty male Albino rats were subjected to diabetes induction and after 1 week the animals were classified into 3 groups, Group I: rats were subjected to tongue ulcer and did not receive any treatment. Group II: rats were subjected to tongue ulcer and the ulcers were treated with 3 times/ day topical applications of commercially used 0.2% high molecular weight hyaluronic acid. Group III: rats were received 1-2 IU/ g/day of insulin NPH injected subcutaneous and then subjected to tongue ulcer after one week from the starting of insulin injection. The animals were sacrificed at 4 and 10 days after ulcer induction, specimens were prepared for histological examination and immunohistochemical staining for α-SMA.Results: Histological examination revealed better enhancement of ulcer healing and reepithelization together with reorganization of the epithelium and connective tissue in group II and III samples when compared to group I after 4 and 10 days of ulcer induction. Conclusion:oral application of hyaluronic acid gel has beneficial effects in accelerating wound healing and improving all phases of wound repair in tongue ulcers in rats.

Prophylactic effect of chitosan-coated ginger nanoparticles versus ginger extract on tongue wound healing in rats exposed to electronic cigarette smoke

“…The tongue is the preferred location for oral squamous cell carcinomas, which account for more than 90% of malignant tumors diagnosed in the oral cavity [29] . Hence, many studies used tongue as a model to study wound healing as, Moawad and Abobaker [30] studied the effect of hyaluronic acid on induced tongue ulcer in diabetic rats. Also, tongue was widely used as an experimental model of oral mucositis that was induced by the combined use of chemotherapeutic agents and radiation [31] .…”

“…However, tongue was considered simple, effective and easily reproducible, model that can be used to assess the prophylactic effects of different agents in the healing process of oral mucosa. The follow-up periods were chosen according to the previously studied spontaneous healing of rat incisional wounds that was reported to occur within two weeks after induction [30] . Consequently, follow-up periods of 7 and 14 days were selected to track the healing process.…”