Impact of<i>Bacillus cereus</i>NRKT on grape ripe rot disease through resveratrol synthesis in berry skin

Aoki, Takanori; Aoki, Yoshinao; Ishiai, Shiho; Otoguro, Misa; Suzuki, Shunji

doi:10.1002/ps.4283

Cited by 16 publications

(14 citation statements)

References 22 publications

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“…Specialized (secondary) metabolism was also strongly influenced by disease onset. Twenty-two upregulated CDS for chalcone and stilbene synthases, involved in phytoalexin production against microbes ( Aoki et al, 2016 ), and plant defense against abiotic stress such as UV-radiation were highlighted in our dataset, with VIT_16s0100g01040 most intensively, and others in the same genomic vicinity of chromosome 16. Terpene metabolism was also modified according to our data, with increased abundance of transcripts for terpene synthases VIT_18s0001g04120 and VIT_00s0692g00020, while reducing that of 10 others with VIT_19s0014g04930 and VIT_00s0271g00010 most intensively.…”

Section: Resultsmentioning

confidence: 99%

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

et al. 2018

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Pierce’s disease is a major threat to grapevines caused by the bacterium Xylella fastidiosa. Although devoid of a type 3 secretion system commonly employed by bacterial pathogens to deliver effectors inside host cells, this pathogen is able to influence host parenchymal cells from the xylem lumen by secreting a battery of hydrolytic enzymes. Defining the cellular and biochemical changes induced during disease can foster the development of novel therapeutic strategies aimed at reducing the pathogen fitness and increasing plant health. To this end, we investigated the transcriptional, proteomic, and metabolomic responses of diseased Vitis vinifera compared to healthy plants. We found that several antioxidant strategies were induced, including the accumulation of gamma-aminobutyric acid (GABA) and polyamine metabolism, as well as iron and copper chelation, but these were insufficient to protect the plant from chronic oxidative stress and disease symptom development. Notable upregulation of phytoalexins, pathogenesis-related proteins, and various aromatic acid metabolites was part of the host responses observed. Moreover, upregulation of various cell wall modification enzymes followed the proliferation of the pathogen within xylem vessels, consistent with the intensive thickening of vessels’ secondary walls observed by magnetic resonance imaging. By interpreting the molecular profile changes taking place in symptomatic tissues, we report a set of molecular markers that can be further explored to aid in disease detection, breeding for resistance, and developing therapeutics.

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Section: Resultsmentioning

confidence: 99%

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

et al. 2018

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“…Bearing in mind that the application of micro‐organisms to grapevine is simple and easy, we have isolated resveratrol synthesis promoting bacteria and characterized their ability to promote resveratrol synthesis in grape berry (Aoki et al . ). We recently demonstrated that the application of B. cereus isolate NRKT to grapevine promoted resveratrol synthesis in berry skin through the activation of the gene expression of stilbene synthase, a key enzyme for resveratrol synthesis (Aoki et al .…”

Section: Activation Of Polyphenol Synthesis In Grapevine By Micro‐orgmentioning

confidence: 97%

“…We recently demonstrated that the application of B. cereus isolate NRKT to grapevine promoted resveratrol synthesis in berry skin through the activation of the gene expression of stilbene synthase, a key enzyme for resveratrol synthesis (Aoki et al . ). The use of NRKT in vineyards is expected to contribute to the increase of resveratrol content in berry skin.…”

Section: Activation Of Polyphenol Synthesis In Grapevine By Micro‐orgmentioning

confidence: 97%

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Status and future of disease protection and grape berry quality alteration by micro-organisms in viticulture

Otoguro

Suzuki

2018

Letters in Applied Microbiology

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Significance and Impact of the Study: In this review, we evaluate the applicability of micro-organisms in viticulture. Micro-organisms can improve grape berry quality through grapevine disease protection and grape berry quality alteration. Because the use of micro-organisms to protect grapevine from plant diseases is safer than the use of chemical fungicides, the use of biofungicides in viticulture is expected to be enhanced by the increasing consumer concern towards chemical fungicides. Micro-organisms also modify plant secondary metabolites for use as flavours, pharmaceuticals and food additives. Studies of micro-organisms that promote polyphenol, anthocyanin and aroma compound biosynthesis are in progress with an eye to improving grape berry quality. AbstractGrapevine is one of the most widely grown fruit crops in the world. At present, however, there is much concern regarding chemical pollution in viticulture due to the application of chemical fungicides and fertilizers. One viticultural practice to resolve this issue is the application of micro-organisms to grapevine as a substitute for chemicals. Some micro-organisms act as an enhancer of grape berry quality as well as a suppresser of disease in grapevine through their antagonistic ability and/or systemic resistance inducing ability. Herein, we review current and prospective applications of micro-organisms in viticulture.

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“…It has been of particular scientific interest to investigate methods of controlling table grape bunch rot diseases. Due to the growing public interest in consuming safe food with the least possible environmental impact, restriction of fungicides, maximum residue limits and the frequent development of resistant strains of B. cinerea [7,8] to synthetic fungicides, biological products have acquired great importance [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes

et al. 2021

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Gray and summer bunch rot are important diseases of table grapes due to the high economic and environmental cost of their control with synthetic fungicides. The ability to produce antifungal compounds against the causal agents Botrytis, Aspergillus, Penicillium, and Rhizopus of two microorganisms isolated from table grapes and identified as Hanseniaspora osmophila and Gluconobacter cerinus was evaluated. In dual cultures, both biocontrol agents (together and separately) inhibited in vitro mycelial growth of these pathogens. To identify the compounds responsible for the inhibitory effect, extractions were carried out with organic solvents from biocontrol agents separately. Through dual cultures with pathogens and pure extracts, only the hexane extract from H. osmophila showed an inhibitory effect against Botrytis cinerea. To further identify these compounds, the direct bioautography technique was used. This technique made it possible to determine the band displaying antifungal activity at Rf = 0.05–0.2. The compounds present in this band were identified by GC-MS and compared to the NIST library. The most abundant compounds, not previously reported, corresponded to alkanes, ketones, alcohols, and terpenoids. H. osmophila and G. cerinus have the potential to control the causal agents of gray and summer bunch rot of table grapes.

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Impact ofBacillus cereusNRKT on grape ripe rot disease through resveratrol synthesis in berry skin

Abstract: The potential use of NRKT in vineyards is expected to contribute to the increase in resveratrol content in berry skins, thereby protecting grape berries against fungal diseases. © 2016 Society of Chemical Industry.

Cited by 16 publications

References 22 publications

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

Molecular Profiling of Pierce’s Disease Outlines the Response Circuitry of Vitis vinifera to Xylella fastidiosa Infection

Status and future of disease protection and grape berry quality alteration by micro-organisms in viticulture

Diffusible Compounds Produced by Hanseniaspora osmophila and Gluconobacter cerinus Help to Control the Causal Agents of Gray Rot and Summer Bunch Rot of Table Grapes

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