Impact of SLC43A3/ENBT1 Expression and Function on 6-Mercaptopurine Transport and Cytotoxicity in Human Acute Lymphoblastic Leukemia Cells

Abstract

ALL -acute lymphoblastic leukemia DMEM -Dulbecco's modified eagle medium DMSO -dimethyl sulfoxide D-PBS -Dulbecco's phosphate buffered saline DY -dipyridamole ENBT1 -equilibrative nucleobase transporter 1 ENT1 -equilibrative nucleoside transporter 1 ENT2 -equilibrative nucleoside transporter 2 ENT4 -equilibrative nucleoside transporter 4 FBS -fetal bovine serum G418 -geneticin GAPDH -glyceraldehyde 3-phosphate dehydrogenase GFP -green fluorescent protein HEK293 -human embryonic kidney 293 HPRT -hypoxanthine-guanine phosphoribosyltransferase HRP -horseradish perdoxidase IMDM -Iscove's modified Dulbecco medium MRP -multidrug resistance protein This article has not been copyedited and formatted. The final version may differ from this version.

Amino acid transporters within the solute carrier superfamily: Underappreciated proteins and novel opportunities for cancer therapy

“…In tumors harboring the fusion gene, more rapid tumor progression and shortened duration between symptoms' unset and the first hospital visit were reported [ 219 ]. Also, another study found that SLC43A3 -encoded equilibration nucleobase transporter 1 (ENBT1) is responsible for importing 6-mercaptopurine, an agent used for the treatment of acute lymphoblastic leukemia, into leukemia cells [ 220 ]. In addition to the SLCs, some transporters are not studied well in cancer ( Table 2 ), and future studies could reveal their critical function in tumorigenesis.…”