Impact of IL10, MTP, SOD2, and APOE Gene Polymorphisms on the Severity of Liver Fibrosis Induced by HCV Genotype 4

Hemeda, Amr Ali; Mohamed, Azah; Aziz, Ramy K.; Abdel-Hakeem, Mohamed S.; Ali-Tammam, Marwa

doi:10.3390/v13040714

Cited by 2 publications

(1 citation statement)

References 60 publications

(68 reference statements)

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“…Hepatitis C is a frequent liver disease found worldwide. Even in the era of DAAs, the determination of genetic markers is important to identify individuals at higher risk of severe disease and to guide therapeutic decisions prior to therapy in patients with chronic hepatitis C. 22 In the present study, the authors analyzed the effect of four candidate SNPs in the MTTP gene combined with host and viral characteristics on hepatic steatosis in a group of chronic hepatitis C patients. Multiple logistic regression analysis for interaction showed an 11.51-fold higher risk of steatosis in patients with the GT/TT genotype of the -493G/T SNP and HCV genotype 3 infections.…”

Section: Discussionmentioning

confidence: 99%

Effect of MTTP -493G/T, I128T, Q95H and Q244E polymorphisms on hepatic steatosis in patients with chronic hepatitis

Prata

Manchiero

Dantas

et al. 2022

Clinics

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Section: Discussionmentioning

confidence: 99%

Effect of MTTP -493G/T, I128T, Q95H and Q244E polymorphisms on hepatic steatosis in patients with chronic hepatitis

Prata

Manchiero

Dantas

et al. 2022

Clinics

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Superoxide dismutase 2 as a predictor in patients with hepatitis B virus-associated acute-on-chronic liver failure

et al. 2023

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The prognosis of hepatitis B virus-associated acute-on-chronic liver failure (HBV-ACLF) is critical in clinical management. We aimed to assess the prognostic e cacy of superoxide dismutase 2 (SOD2) for 90-day mortality in HBV-ACLF patients. The expression patterns of SOD2 in peripheral blood mononuclear cells (PBMCs) were examined in a derivation set (n = 82) by quantitative real-time polymerase chain reaction (RT-qPCR). The results were further validated in a validation set (n = 35). The expression levels of SOD2 were signi cantly decreased in the derivation set compared to those with chronic hepatitis B (CHB) or the healthy controls (HCs) (P < 0.001). In HBV-ACLF patients, SOD2 levels were negatively correlated with serum total bilirubin (TBIL) (rs = -0.43, P < 0.001) and model for end-stage liver disease (MELD) scores (rs = -0.22, P = 0.047), but positively correlated with alkaline phosphatase (AKP) (rs = 0.23, P = 0.034). SOD2 was identi ed as an independent risk factor for 90-day mortality in HBV-ACLF patients (hazard ratio: 0.124, 95% con dence interval: 0.059-0.261, P < 0.001). SOD2 yielded a larger area under the receiver operating characteristic curve (AUROC) than the MELD score in predicting 90-day mortality (0.914 versus 0.712, P < 0.001). Kaplan-Meier analysis revealed a favorable overall survival (OS) for the SOD2 high expression group compared with the SOD2 low expression group in both the derivation and validation sets (P < 0.001). SOD2 has promising potential as a predictor of 90-day mortality in patients with HBV-ACLF. synthesis kit (Fermentas, Vilnius, Lithuania). qRT-PCR was performed using SYBR® Premix ExTaqTM (TaKaRa), with β-actin serving as the internal control. The reaction was performed in a total volume of 10 µL, which included 4.1 µL of RNase-free ddH2O, 0.2 µL of speci c forward and reverse primers, 5 µL of SYBR Green, and 0.5 µL of cDNA, under the following conditions: initial denaturation at 95℃ for 10 minutes, then 40 cycles of denaturation at 95 °C for 5 seconds, annealing at 58 °C for 30 seconds and extension at 72 °C for 30 seconds. The primers used have been described in a previous study [17]: SOD2 forward, 5′-GAGAAGTACCAGGAGGCGTTG-3′; SOD2 reverse, 5′-GAGCCTTGGACACCAACAGAT-3′; β-actin forward, 5′-AGAGCTACGAGCTGCCTGAC-3′; β-actin reverse, 5′-AGCACTGTGTTGGCGTACAG-3′. The relative expression of SOD2 was calculated using the 2 − ΔΔCT method. All ampli cation reactions were performed in triplicate. Statistical AnalysisStatistical analyses were performed using IBM SPSS Statistics (version 26.0; IBM Statistics, Armonk, NY) and R software (version 4.0.2 for Windows; the R foundation for statistical computing, Vienna, Austria). The demographic characteristics are presented as medians (interquartile range) or percentages. The Mann-Whitney U test was used to quantitative variables, while the chi-square test was used to categorical variables. Spearman rank correlation analysis was used to assess the associations between SOD2 levels and clinical variables. Using Cox proportional hazard regr...

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Impact of IL10, MTP, SOD2, and APOE Gene Polymorphisms on the Severity of Liver Fibrosis Induced by HCV Genotype 4

Cited by 2 publications

References 60 publications

Effect of MTTP -493G/T, I128T, Q95H and Q244E polymorphisms on hepatic steatosis in patients with chronic hepatitis

Effect of MTTP -493G/T, I128T, Q95H and Q244E polymorphisms on hepatic steatosis in patients with chronic hepatitis

Superoxide dismutase 2 as a predictor in patients with hepatitis B virus-associated acute-on-chronic liver failure

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