Impact of industrial dry-milling on fumonisin redistribution in non-transgenic corn in Brazil

Bordini, Jaqueline Gozzi; Ono, Mário Augusto; Garcia, Glauco Tironi; Fazani, Victor Hugo Meconi; Vizoni, Édio; Rodrigues, Karem Caroline Bonacin; Hirooka, Elisa Yoko; Ono, Elisabete Yurie Sataque

doi:10.1016/j.foodchem.2016.10.028

Cited by 14 publications

(5 citation statements)

References 32 publications

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“…The low fumonisin contamination in endosperm, cornmeal and grits was due to the industrial processing that removed the outer parts of the corn grain (germ and pericarp) to produce corn-based products intended for human consumption from the corn endosperm. It has been reported that germ and pericarp are the corn fractions most contaminated by mycotoxins [29,30,31].…”

Section: Resultsmentioning

confidence: 99%

Safety of Corn and Corn-Based Products Intended for Human Consumption Concerning Fumonisins from a Brazilian Processing Plant

Bordini

Ono

Hirozawa

et al. 2019

Toxins

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Brazil is one of the world’s largest corn producers and is a leader in exportation. Due to intense globalization, corn may be commercialized worldwide and the issue concerning the safety of corn-based products has become a topic of widespread international interest. Dietary exposure evaluation is a relevant criterion for mycotoxin risk assessment. Thus, human exposure to fumonisins were assessed for corn grain and its derivatives (endosperm, cornmeal, and grits; n = 320) sampled from one of the large-scale corn processing plants in Brazil. The total probable daily intake (PDI) for fumonisins in Brazil was 96.9 ng kg−1 body weight day−1, which corresponds to 5% of the provisional maximum tolerable daily intake (PMTDI) of 2000 ng kg−1 b.w. day−1 for fumonisins. In countries that import Brazilian corn, the total PDI is lower in European countries (from 35.7 to 177 ng kg−1 b.w. day−1) and higher in Angola (1553 ng kg−1 b.w. day−1). Taking into account that dietary exposure in populations in Brazil and importing countries was low, the corn-based products were safe for human consumption regarding fumonisins, even for regions with high corn consumption.

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Section: Resultsmentioning

confidence: 99%

Safety of Corn and Corn-Based Products Intended for Human Consumption Concerning Fumonisins from a Brazilian Processing Plant

Bordini

Ono

Hirozawa

et al. 2019

Toxins

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“…In the current method where whole maize kernels are utilised, the rate of FB hydrolysis is determined by substrate availability (Alberts, Schatzmayr, et al, 2019). Depending on the fungal damage, FB could colonise different layers of the kernel (Brera et al, 2004;Duncan & Howard, 2010) and therefore, the FB level associated with each milling product will depend on the extent of fungal damage of the surface and inner layers (Bordini et al, 2017;Burger et al, 2013). In the current dry milling experimental model, complete FB 1 hydrolysis was only achieved at 32 U/100 g maize reaching a 1:1 conversion into HFB 1 .…”

Section: Tablementioning

confidence: 85%

Enzymatic detoxification of the fumonisin mycotoxins during dry milling of maize

Alberts

Davids

Moll³

et al. 2021

Food Control

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Fumonisin esterase FumD (EC 3.1.1.87), FUMzyme® (BIOMIN, Austria), effectively detoxifies fumonisin B mycotoxins (FB) by hydrolysis and removal of the tricarballylic acid groups. The current study evaluated FumD detoxification of total FB (FB T ) in commercial maize utilising an experimental dry milling plant by introducing the enzyme during the kernel conditioning stage. Total FB and the hydrolysed product of FB 1 , HFB 1 , in maize and milling products were determined by LC-MS/MS. During maize conditioning of 4 h 10 min substantial FB 1 hydrolysis was achieved between 1 and 4 U FumD/100 g maize. Complete conversion into HFB 1 was delayed and only achieved at the highest enzyme concentration (32 U/100 g maize) reaching a 1:1 M conversion ratio. Dry milling of maize containing 3.29 ± 0.20 μmole FB T /kg (2354 ± 140 μg/kg) in the absence of FumD, resulted in a 2.5-fold increase in the FB T concentration in total hominy feed (8.34 ± 0.22 μmol/kg) (5979 ± 158 μg/kg) compared to the levels that prevail in Super (0.52 ± 0.07 μmol/kg) (347 ± 48 μg/kg) and Special (1.70 ± 0.01 μmol/kg) (1213 ± 8 μg/kg) maize meal, and Semolina (1.07 ± 0.14 μmol/kg) (765 ± 100 μg/kg) milling products. Introduction of FumD (40 U/kg) mainly impacted the total hominy feed product (germ + hominy milling fractions), constituting up to 30% of the reconstituted whole maize. A 99% reduction in FB T was obtained in total hominy feed, 48% in Semolina, 7% in Special maize meal, whereas no reduction was recorded in Super maize meal. FB 1 reduction rates depend on the contamination level, kernel moisture and the diffusion rate from inner kernel layers to the kernel surface/aqueous interface. Risk modelling in children and adults indicated that FumD-treated whole maize and the resultant Semolina milling product intended for human consumption reduces the risk of exposure to FB T . However, no reduction in the exposure risk was observed when considering the Super and Special maize meal milling products. FB reduction in total hominy feed could open up new applications, such as its dietary incorporation as a source of fibre, minerals and bioactive plant constituents in maize-based food. In addition, the animal feed industry and subsistence maize farming communities using rudimentary milling processes, could also benefit.

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“…Fumonisins B 1 and B 2 in poultry feed samples were determined by HPLC, according to Bordini et al [11].…”

Section: Methodsmentioning

confidence: 99%

“…Natural occurrence of F. verticillioides and fumonisins in corn and corn-based feed is a worldwide problem [6,7,8,9,10,11].…”

Section: Introductionmentioning

confidence: 99%

Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

Omori

Ono

Hirozawa

et al. 2019

Toxins

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Fumonisins are a group of toxic secondary metabolites that are produced by Fusarium verticillioides which are associated with poultry health hazard and great economic losses. The objective of the present study was to develop an immunological method to detect F. verticillioides in poultry feed samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) based on a polyclonal antibody against 67 kDa protein of the F. verticillioides 97K exoantigen was developed to detect this fungus. Antibody anti-67 kDa protein showed cross-reactivity against F. graminearum (2–7%) and F. sporotrichioides (10%), but no or low cross-reactivity against Aspergillus sp. and Penicillium sp. exoantigens. The detection limit for the 67 kDa protein of F. verticillioides was 29 ng/mL. Eighty-one poultry feed samples were analyzed for Fusarium sp. count, 67 kDa protein of F. verticillioides and fumonisin concentrations. Eighty of the 81 feed samples (98.6%) showed Fusarium sp. contamination (mean 6.2 x 104 CFU/g). Mean 67 kDa protein and fumonisin concentration in the poultry feed samples was 21.0 µg/g and 1.02 µg/g, respectively. The concentration of 67 kDa protein, as determined by ic-ELISA correlated positively (p < 0.05) with fumonisin levels (r = 0.76). These results suggest that this ic-ELISA has potential to detect F. verticillioides and predict fumonisin contamination in poultry feed samples.

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Impact of industrial dry-milling on fumonisin redistribution in non-transgenic corn in Brazil

Cited by 14 publications

References 32 publications

Safety of Corn and Corn-Based Products Intended for Human Consumption Concerning Fumonisins from a Brazilian Processing Plant

Safety of Corn and Corn-Based Products Intended for Human Consumption Concerning Fumonisins from a Brazilian Processing Plant

Enzymatic detoxification of the fumonisin mycotoxins during dry milling of maize

Development of Indirect Competitive Enzyme-Linked Immunosorbent Assay to Detect Fusarium verticillioides in Poultry Feed Samples

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