Background: Chinese nurses working with immense stress may have issues with burnout during COVID-19 regular prevention and control. There were a few studies investigating status of burnout and associated factors among Chinese nurses. However, the relationships remained unclear.Objectives: To investigate status and associated factors of nurses' burnout during COVID-19 regular prevention and control. Methods: 784 nurses completed questionnaires including demographics, Generalized Anxiety Disorder-7, Patient Health Questionnaire-9, Insomnia Severity Index, Impact of Event Scalerevised, Perceived Social Support Scale, Connor-Davidson Resilience Scale, General Self-efficacy Scale and Maslach Burnout Inventory. Results: 310 (39.5%), 393 (50.1%) and 576 (73.5%) of respondents were at high risk of emotional exhaustion (EE), depersonalization (DP) and reduced personal accomplishment (PA). The risk of EE, DP and reduced PA were moderate, high and high. Nurses with intermediate and senior professional rank and title and worked >40 h every week had lower scores in EE. Those worked in low-risk department reported lower scores in PA. Anxiety, post-traumatic stress disorder (PTSD), self-efficacy and social support were influencing factors of EE and DP, while social support and resilience were associated factors of PA. Conclusion: Chinese nurses' burnout during COVID-19 regular prevention and control was serious. Professional rank and title, working unit, weekly working hours, anxiety, PTSD, selfefficacy, social support and resilience were associated factors of burnout. Impact statement Chinese nurses seemed to be more likely to suffer from burnout during COVID-19 regular prevention and control, since they worked in different conditions of clinical settings and were closely related to COVID-19 patient care, which put high physical and psychological pressure on nurses. Understanding the status of burnout of nursing group and associated factors during COVID-19 regular prevention and control is of great importance. It might aid the Chinese hospital management with potential measures to reduce or even prevent burnout among nurses during COVID-19 regular prevention and control. However, studies concerning the burnout and its associated factors are still limited. This study has examined the prevalence and associated factors of burnout among nurses during COVID-19 regular prevention and control of COVID-19 in Jiangsu Province, China. Background Burnout is a syndrome of emotional exhaustion (EE) and cynicism, often occurring in people who are engaged in "people-work" (Maslach and Jackson, 1981). It is usually measured by the Maslach Burnout Inventory, which has three dimensions (EE, depersonalization [DP] and personal accomplishment [PA]) (Genly, 2016). Several theories were proposed to understand Cambridge Prisms: Global Mental Health www.cambridge.org/gmh