Impact of interleukin-6 gene polymorphisms and its interaction with obesity on osteoporosis risk in Chinese postmenopausal women

Abstract

Aims To investigate the association of four single-nucleotide polymorphisms (SNPs) of the IL-6 gene with osteoporosis (OST) susceptibility. Methods PCR restriction fragment length polymorphism (PCR–RFLP) was carried out for SNPs detection. Generalized multifactor dimensionality reduction (GMDR) model and logistic regression model were used to examine the interaction between SNP and obesity on OST. Results Logistic regression model revealed that G allele of rs1800796 and the T allele of rs2069849 were associated with increased OST risk, compared to those with wild genotype. However, no significant correlations were found when analyzing the association of rs1800795 and rs1554606 with OST risk. GMDR analysis suggested that the interaction model composed of the rs1800796 and obesity was the best model with statistical significance ( P value from sign test [ P sign ] = 0.012), indicating a potential gene–environment interaction between rs1800796 and obesity. Overall, the two-locus models had a cross-validation consistency of 10/10 and had the testing accuracy of 0.641. We also conducted stratified analysis for rs1800796 genotype and obesity, and found that obese subjects with CG or GG genotype have the highest OST risk, compared to subjects with CC genotype, and normal BMI OR (95% CI) = 2.21 (1.52–3.49), after adjustment for age, smoke, and alcohol consumption status. Conclusions Our results suggested that the C allele of rs1800796 and the C allele of rs2069849 of IL-6 gene interaction between rs1800796 and abdominal obesity were all associated with increased OST risk.

Association of single nucleotide polymorphisms of IL-6 gene with frailty in the Chinese Bai nationality

“…For instance, genes such as −572G/C (rs1800796) of G weaken skeletal muscle, which limits everyday physical activity in the elderly and makes them clumsy, which in turn accelerates muscle loss and physiological dysfunction and is an important variable in frailty. [28] IL6-related genomic polymorphisms can be used as genetic markers for several cardiovascular diseases, and IL-6 −572C/G and −174G/C polymorphisms are closely associated with atherosclerosis, hypertension, cerebral infarction, coronary artery disease, and other cardiovascular diseases, in which the G allele in −572C/G and the C allele in −174G/C increase their risk of disease and accelerate the development of frailty processes. [7,29,30] According to some studies, rs1800796 GG may increase the risk of developing type 2 diabetic nephropathy and may also be related to lumbar intervertebral disc degeneration.…”

Use of Network Pharmacology and Molecular Docking Technology to Analyze the Mechanism of Action of Velvet Antler in the Treatment of Postmenopausal Osteoporosis

“…A significant feature of PMOP is chronic inflammation, and the increase in inflammatory factors such as tumor necrosis factor (TNF) and IL-6 can augment the expression of RANKL [ 46 ]. IL-6 affects bone loss and osteoporosis [ 47 ] and plays an important role in bone reconstruction [ 48 ]. MAPK3 (also known as ERK1) is a member of the MAPK-signaling pathway; studies have found that activated MAPK can reduce the differentiation of osteoclasts, promote the generation of osteoblasts, and improve osteoporosis [ 49 ].…”

Identification of the specific microRNAs and competitive endogenous RNA mechanisms in osteoporosis

“…Inflammation is a contributing factor to the development of postmenopausal osteoporosis. 35 , 36 Elevated circulating levels of IL-1β, TNF-α, and IL-6 are positively associated with bone destruction, bone loss, and excessive bone resorption. 10 , 15 , 16 , 35 , 37 The canonical Wnt/β-catenin pathway is an osteoblastic pathway.…”