Impact of ionic strength of growth on the physiochemical properties, structure, and adhesion of Listeria monocytogenes polyelectrolyte brushes to a silicon nitride surface in water

Gordesli, Fatma Pinar; Abu-Lail, Nehal I.

doi:10.1016/j.jcis.2012.08.048

Cited by 14 publications

(15 citation statements)

References 69 publications

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“…It is worth noting that these studies did not investigate the mechanical properties of MDR bacterial cells. Irrespective of their shortcomings, these investigations highlighted the possible means through which bacteria respond to antibiotics and explored how various AFM modes and theoretical models can be employed to estimate bacterial mechanical properties in different environmental conditions ( Atabek and Camesano, 2007 , Perry et al, 2009 , Gordesli and Abu-Lail, 2012 , Longo et al, 2013a , Longo et al, 2013b ).…”

Section: Discussionmentioning

confidence: 99%

Changes in cellular elasticities and conformational properties of bacterial surface biopolymers of multidrug-resistant Escherichia coli (MDR-E. coli) strains in response to ampicillin

Uzoechi

Abu-Lail

2019

The Cell Surface

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The roles of the thicknesses and grafting densities of the surface biopolymers of four multi-drug resistant (MDR) Escherichia coli bacterial strains that varied in their biofilm formation in controlling cellular elasticities after exposure to ampicillin were investigated using atomic force microscopy. Exposure to ampicillin was carried out at minimum inhibitory concentrations for different duration times. Our results indicated that the four strains resisted ampicillin through variable mechanisms. Strain A5 did not change its cellular properties upon exposure to ampicillin and as such resisted ampicillin through dormancy. Strain H5 increased its biopolymer brush thickness, adhesion and biofilm formation and kept its roughness, surface area and cell elasticity unchanged upon exposure to ampicillin. As such, this strain likely limits the diffusion of ampicillin by forming strong biofilms. At three hours’ exposure to ampicillin, strains D4 and A9 increased their roughness, surface areas, biofilm formation, and brush thicknesses and decreased their elasticities. Therefore, at short exposure times to ampicillin, these strains resisted ampicillin through forming strong biofilms that impede ampicillin diffusion. At eight hours’ exposure to ampicillin, strains D4 and A9 collapsed their biopolymers, increased their apparent grafting densities and increased their cellular elasticities. Therefore, at long exposure times to ampicillin, cells utilized their higher rigidity to reduce the diffusion of ampicillin into the cells. The findings of this study clearly point to the potential of using the nanoscale characterization of MDR bacterial properties as a means to monitor cell modifications that enhance “phenotypic antibiotic resistance”.

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Section: Discussionmentioning

confidence: 99%

Changes in cellular elasticities and conformational properties of bacterial surface biopolymers of multidrug-resistant Escherichia coli (MDR-E. coli) strains in response to ampicillin

Uzoechi

Abu-Lail

2019

The Cell Surface

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“…When this situation occurs, the cell is stable at a separation distance d sm from the surface (reversible adhesion), however if the cells can overcome the energy barrier constituted by the local maximum, another stable situation can be reached (irreversible adhesion). Energy barriers up to 800 k b T have been shown surmountable for bacteria to reach primary minimum position; alsoadhesion counts in the primary minimum increased with lowering energy barriers [83]- [85]. The switch from one situation to another can be the results of Brownian motion or the formation of cell surface features (fimbrie, curli and other adhesion exopolymer) that act like a bridge between cell and substrate [54], [83].…”

Section: Application Of Dlvo To Biofilmsmentioning

confidence: 99%

“…In order to apply the DLVO theory to this situation, the equations for the van der Waals and electrostatic forces between two dissimilar spheres are used. Such investigations have allowed the elucidation of the role of ionic strength [85] and growth temperature [118], [120] on Listeria monocytogenes adhesion properties.…”

Section: Estimation Of the Interaction Forcesmentioning

confidence: 99%

Success and failure of colloidal approaches in adhesion of microorganisms to surfaces

Perni

Preedy²,

Prokopovich

2014

Advances in Colloid and Interface Science

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Biofilms are communities of cells attached to surfaces, their contributions to biological process may be either a benefit or a threat depending on the microorganism involved and on the type of substrate and environment. Biofilm formation is a complex series of steps; due to the size of microorganisms, the initial phase of biofilm formation, the bacterial adhesion to the surface, has been studied and modeled using theories developed in colloidal science. In this review the application of approaches such as Derjaguin, Landau, Verwey, Overbeek (DLVO) theory and its extended version (xDLVO), to bacterial adhesion is described along with the suitability and applicability of such approaches to the investigation of the interface phenomena regulating cells adhesion. A further refinement of the xDLVO theory encompassing the brush model is also discussed. Finally, the evidences of phenomena neglected in colloidal approaches, such as surface heterogeneity and fluid flow, likely to be the source of failure are defined.

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“…To date, many previous studies have shown that microbial adhesion onto abiotic surfaces can be influenced by multiple factors, such as the surface properties of microorganism and abiotic surfaces, including their surface free energy (SFE), ζ potential, hydrophobicity, roughness, and morphology, , the characteristics of the cultivating liquid medium, including its surface tension, pH, ionic strength, temperature and hydrodynamics, , and some biotic factors, including the properties of cell membrane, production of extracellular polymeric substances, the chemotaxis of cells, and the presence of pili and flagella. , Overall, it has been established that these factors can influence cell adhesion mainly by affecting the physicochemical interactions between cells and surfaces.…”

Section: Introductionmentioning

confidence: 99%

Quantitative Criterion to Predict Cell Adhesion by Identifying Dominant Interaction between Microorganisms and Abiotic Surfaces

et al. 2018

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Cell adhesion is ubiquitous and plays an important role in various scientific and engineering problems. Herein, a quantitative criterion to predict cell adhesion was proposed by identifying the dominant interaction between microorganisms and abiotic surfaces. According to the criterion, the dominant interaction in cell adhesion could be identified as a Lewis acid–base (AB) interaction or electrostatic (EL) interaction via comparison of two expressions containing the electron-donor characteristics of the microorganism (γmv –) and abiotic surface (γsv –) and their ζ potentials (ζm, ζs). The results revealed that when dominated by the AB interaction, adhesion would decrease with increasing . However, when the EL interaction was dominant, adhesion would decrease with increasing (ζm + ζs)2. We have verified the criterion based on the adhesion of microalgae, bacteria, and fungi onto various surfaces obtained via our experiments and available in literature studies. The results demonstrated that the criterion had important implications in the prediction of cell adhesion in various applications.

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Impact of ionic strength of growth on the physiochemical properties, structure, and adhesion of Listeria monocytogenes polyelectrolyte brushes to a silicon nitride surface in water

Cited by 14 publications

References 69 publications

Changes in cellular elasticities and conformational properties of bacterial surface biopolymers of multidrug-resistant Escherichia coli (MDR-E. coli) strains in response to ampicillin

Changes in cellular elasticities and conformational properties of bacterial surface biopolymers of multidrug-resistant Escherichia coli (MDR-E. coli) strains in response to ampicillin

Success and failure of colloidal approaches in adhesion of microorganisms to surfaces

Quantitative Criterion to Predict Cell Adhesion by Identifying Dominant Interaction between Microorganisms and Abiotic Surfaces

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